Anti foxa1 ab23738

Manufactured by Abcam

Anti-FOXA1 (ab23738) is a rabbit monoclonal antibody targeting the FOXA1 protein. FOXA1 is a member of the forkhead box (FOX) family of transcription factors. The antibody is suitable for use in various applications, including western blotting, immunohistochemistry, and immunocytochemistry.

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Lab products found in correlation

0.22 mm nitrocellulose nc membranes, by Merck Group (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Roche (1 mentions) Anti gata2 sc 9008, by Santa Cruz Biotechnology (1 mentions) Anti h3k27ac 39133, by Active Motif (1 mentions) Lncap cell, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

Anti-FOXA1 (ab23738) and anti-GAPDH (ab9385) FOXA1 (ab23738), Anti-FOXA1 Ab23738 FOXA1

2 protocols using anti foxa1 ab23738

Western Blot Analysis of Protein Markers

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The cells were lysed using mammalian protein extraction reagent radioimmunoprecipitation assay lysis buffer (RIPA; Beyotime, Haimen, China), supplemented with protease inhibitor cocktail (Roche) and PMSF (Roche). 50 μg of the protein extractions was separated by 10% SDS-PAGE, transferred to 0.22-mm nitrocellulose (NC) membranes (Sigma-Aldrich), and incubated with specific antibodies. The autoradiograms were quantified by densitometry (Quantity One software). Anti-FOXA1 (ab23738), Anti-BIK (ab52182), and Anti-XAF1 (ab17204) were from Abcam. Results were normalized to the expression of GAPDH.

Xu T., Yan S., Jiang L., Yu S., Lei T., Yang D., Lu B., Wei C., Zhang E, & Wang Z. (2019). Gene Amplification-Driven Long Noncoding RNA SNHG17 Regulates Cell Proliferation and Migration in Human Non-Small-Cell Lung Cancer. Molecular Therapy. Nucleic Acids, 17, 405-413.

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LNCaP Cell Culture and Antibody Usage

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LNCaP cells were obtained from American Type Culture Collection and passaged in our laboratory for less than 6 months after resuscitation. LNCaP cells are maintained in RMPI 1640 culture medium supplemented with 10% fetal bovine serum in a 37°C humidified chamber supplemented with 5% CO₂. Antibodies used in this study include anti- H3K27ac (39133) from Active Motif, anti-FOXA1 (ab23738) from Abcam, anti-AR (sc-815X), anti-GATA2(sc-9008) and anti-HOXB13(sc-66923) from Santa Cruz

Jin H.J., Jung S., DebRoy A.R, & Davuluri R.V. (2016). Identification and validation of regulatory SNPs that modulate transcription factor chromatin binding and gene expression in prostate cancer. Oncotarget, 7(34), 54616-54626.

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