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Spike protein s1 rbd

Manufactured by BPS Biosciences
Sourced in United States

The Spike protein S1 RBD is a laboratory product that represents the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein S1 subunit. This recombinant protein is produced in a eukaryotic expression system and can be used for various research and diagnostic applications.

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3 protocols using spike protein s1 rbd

1

SARS-CoV-2 Spike Inhibitor Screening

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MG was purchased from ChemFaces (Wuhan, China). The SARS-CoV-2 spike/ACE2 inhibitor screening assay kit, biotin-labeled recombinant protein ACE2 receptor, and spike protein S1 RBD (BPS Bioscience, San Diego, CA, USA) were procured for biolayer interferometry. HEK293T cells stably expressing human ACE2 and TMPRSS2 cells were obtained from GeneCopoeia (Rockville, MD, USA) and maintained in Dulbecco’s modified Eagle’s medium (Lonza, Walkersville, MD, USA) containing 10% fetal bovine serum (Biotechnics Research, Lake Forest, CA, USA) and 1% penicillin/streptomycin (Cellgro, Manassas, VA, USA) at 37 °C in a 5% CO2 incubator. Further, wild-type (WT) and mutant (D614G) SARS-CoV-2 spike pseudotyped viruses were purchased from GeneCopoeia (Rockville, MD, USA). Vero cells were purchased from American Type Culture Collection (Manassas, VA, USA). SARS-CoV-2 (βCoV/Korea/KCDC03/2020) was provided by Korea Centers for Disease Control and Prevention (Cheongju, Korea).
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2

SARS-CoV-2 Spike Inhibition Assay

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Geraniin (ChemFaces, Wuhan, China) was purchased. The SARS-CoV-2 spike: ACE2 inhibitor screening assay kit, biotin-labeled recombinant protein ACE2 receptor, and spike protein S1 RBD (BPS Bioscience, San Diego, CA, USA) for biolayer interferometry were procured.
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3

SARS-CoV-2 Spike/ACE2 Inhibitor Screening

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KC was purchased from ChemFaces (Wuhan, China). The SARS-CoV-2 spike/ACE2 inhibitor screening assay kit, biotin-labeled recombinant protein ACE2 receptor, and spike protein S1 RBD (BPS Bioscience, San Diego, CA, USA) were purchased for the competitive ELISA assay and BLItz analysis. Human ACE2/TMPRSS2-overexpressing HEK293T stable cell line was purchased from GeneCopoeia (Rockville, MD, USA) and maintained in Dulbecco’s modified Eagle’s medium (Lonza, Walkersville, MD, USA) containing 10% fetal bovine serum (Biotechnics Research, Lake Forest, CA, USA) and 1% penicillin/streptomycin (Cellgro, Manassas, VA, USA) at 37 °C in a 5% CO2 incubator. WT and mutant (D614G) SARS-CoV-2 spike pseudotyped viruses (cat. no. SP101-100 and SP103-100) were purchased from GeneCopoeia (Rockville, MD, USA). Vero cells were purchased from American Type Culture Collection (Manassas, VA, USA). A clinical isolate of SARS-CoV-2 alpha strain (βCoV/Korea/KCDC03/2020) was provided by Korea Centers for Disease Control and Prevention (Cheongju, Korea).
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