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Penicillin streptomycin

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Penicillin/streptomycin is a combined antibiotic solution commonly used in cell culture applications. It provides broad-spectrum protection against bacterial contamination.

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Lab products found in correlation

Fetal bovine serum (fbs), by Biowest (66 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (43 mentions) L glutamine, by Biowest (31 mentions) Dulbecco modified eagle medium (dmem), by Biowest (15 mentions) Rpmi 1640 medium, by Biowest (13 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (12 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (11 mentions) Non essential amino acids (neaa), by Biowest (10 mentions) Dimethyl sulfoxide (dmso), by Merck Group (10 mentions) Rpmi 1640, by Biowest (9 mentions) Amphotericin b, by Biowest (8 mentions) Fetal bovine serum (fbs), by Merck Group (7 mentions) Dmem f12, by Biowest (7 mentions) Dmem high glucose, by Biowest (7 mentions) Dulbecco s modified eagle s medium dmem, by Biowest (7 mentions) Penicillin streptomycin, by Merck Group (6 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (6 mentions) Trypsin edta, by Biowest (5 mentions) Gentamicin, by Biowest (5 mentions) Glutamax, by Thermo Fisher Scientific (5 mentions)

221 protocols using penicillin streptomycin

1

Isolation and Culture of Mesenchymal Stem Cells and Melanoma Cells

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Human placental mesenchymal stem cells (hpMSCs) were obtained from Cellular Engineering Technologies (CET) (Caralville, IA, USA), while B16-F1 (low metastatic variant) and B16-F10 (high metastatic variant) murine skin melanoma cells, were provided by cell services from Cancer Research-UK. NIH-3T3 murine healthy fibroblasts were obtained from Dr. Antonio de la Vieja’s group (Instituto de Salud Carlos lll). hpMSCs were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Biowest, France) supplemented with 5 μg mL−1 of FGF-2 growth factor (PeproTech, Cranbury, NJ, USA), with 10% of fetal bovine serum (FBS, GIBCO, Waltham, MA, USA), 1% penicillin/streptomycin and 1% amphotericin (Biowest, France) and maintained at 37 °C in a 5% CO2-humidified atmosphere under hypoxic conditions (3% O2). For culturing B16-F1, B16-F10, and NIH-3T3 cells, DMEM with 10% of FBS (GIBCO), supplemented with 1% penicillin/streptomycin and 1% amphotericin (Biowest, France) was used. Finally, monocytes were cultured in RMPI Medium 1640 (Biowest, France) supplemented with 10% FBS (GIBCO), 1% penicillin/streptomycin and 1% amphotericin (Biowest, France). B16-F1, B16-F10 and NIH-3T3 cells were maintained under normoxic conditions.
To obtain culture media free of EVs, they were depleted from serum by ultracentrifugation at 100,000× g for 8 h at 4 °C.
Sancho-Albero M., Martín-Pardillos A., Irusta S., Sebastián V., Cebolla V.L., Pazo-Cid R., Martín-Duque P, & Santamaría J. (2023). X-ray Photoelectron Spectroscopy (XPS) Analysis of Nitrogen Environment in Small Extracellular Vesicle Membranes: A Potential Novel Technique with Application for Cancer Screening. Cancers, 15(9), 2479.
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2

Establishing Breast Cancer and Normal Cell Lines

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Breast cancer cell lines MCF-7 (American Type Culture Collection [ATCC]: HTB-22) and SK-BR-3 (ATCC: HTB-30) were maintained in Dulbecco's modi ed eagle medium (DMEM; BI, 01-355-1A) and McCoy's 5A modi ed medium (BI, 01-075-1A) supplemented with 10% fetal bovine serum (FBS; Stem Cell, 04F11678) and 1% penicillin/streptomycin (Biowest, S12204L0018) in an incubator at 37 o C with 5% CO 2 . MCF-12A (ATCC: CRL-10782) normal breast cells were maintained in DMEM supplemented with Ham's F12 nutrient mixture, 10% FBS (Stem Cell, 04F11678), 1% penicillin/streptomycin (Biowest, S12204L0018), 20 ng/mL of epidermal growth factor (Genetropin, P zer), 0.01 mg/mL of insulin (Sigma, 16634), and 500 ng/mL of hydrocortisone (Gadexon 8 mg, MSpharma) in an incubator at 37 o C with 5% CO 2 . NK-92 (ATCC: CRL-2407) cells were cultured in TexMACS GMP medium (Miltenyi Biotec, 170-076-309) supplemented with 10% FBS (Stem Cell, 04F11678), 1% penicillin/streptomycin (Biowest, S12204L0018), and 500 IU of interleukin (IL)-2 (Proleukin, Novartis) in an incubator at 37 o C with 5% CO 2 .
Kilic N., Dastouri M., Kandemir I, & Yilmaz E. (2023). The effects of KIR2DL4 stimulated NK-92 cells on the apoptotic pathways of HER2 + /HER-breast cancer cells. Medical oncology (Northwood, London, England), 40(5).
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3

Cell Culture Conditions for Colorectal Cell Lines

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NCM460 and SW480 cells were grown in Roswell Park Memorial Institute (RPMI) 1640 medium with stable glutamine (Biowest, Nuaillé, France) supplemented with 1% penicillin-streptomycin (Biowest, Nuaillé, France) and 10% heat-inactivated fetal bovine serum (FBS; Gibco, Invitrogen, Waltham, Massachusetts, USA); RKO cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM) High Glucose (Biowest, Nuaillé, France) supplemented with 1% penicillin-streptomycin (Biowest, Nuaillé, France) and 10% heat-inactivated FBS (Gibco, Invitrogen, Waltham, MA, USA). HCT116 cells were grown in McCoy’s 5A Medium (Biowest, Nuaillé, France) supplemented with 1% penicillin-streptomycin (Biowest) and 10% heat-inactivated FBS (Gibco, Invitrogen, Nuaillé, France). All cell lines were plated onto 25 cm3 tissue culture flasks, maintained in a humidified incubator with 5% CO2 at 37 °C.
Ferreira J.C., Granja S., Almeida A.F., Baltazar F., Gonçalves M.S., Preto A, & Sousa M.J. (2022). Targeting Lysosomes in Colorectal Cancer: Exploring the Anticancer Activity of a New Benzo[a]phenoxazine Derivative. International Journal of Molecular Sciences, 24(1), 614.
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4

Umbilical Cord MSC Treatment Protocols

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Human umbilical cord MSCs were obtained from the IdISBa Biobank. Their use was approved by its Ethics Committee (IB1955/12BIO). MSCs were cultured at 37°C and 5% CO2, using Dulbecco’s Modified Eagle Medium (DMEM)–low glucose (Biowest) with 100 μg/ml penicillin-streptomycin (Biowest) and 20% foetal bovine serum (FBS) embryonic stem cells tested (Biowest). Half of the medium was changed every three days.
For treatment, 30,000 cells/cm2 were seeded, at confluence, two PBS washes were performed and treated: Control (PBS), PL (2.6 μg/cm2 of PL), UC-EVs (2.6 μg/cm2 of UC isolated EVs, equivalent to 3.5 × 106 part/cm2), low dose (LD)-SEC-EVs (3.5 × 106 part/cm2 of SEC isolated EVs), and high dose (HD)-SEC-EVs (3.0 × 1010 part/cm2 of SEC isolated EVs). The treatment of UC-EVs was normalized by the amount of proteins used in the PL group, which was set in preliminary studies (data not shown). For LD-SEC-EVs, the same amount of particles as UC-EVs was used. Finally, a 50-fold amount of proteins versus PL was used for the HD-SEC-EV treatment.
Treatments were given in DMEM Low Glucose (Biowest), 100 μg/ml penicillin-streptomycin, and 1% FBS-EVs depleted by centrifugation at 120,000 xg for 18 hours at 4°C. Medium and treatments were refreshed every three days.
Antich-Rosselló M., Forteza-Genestra M.A., Calvo J., Gayà A., Monjo M, & Ramis J.M. (2020). Platelet-derived extracellular vesicles promote osteoinduction of mesenchymal stromal cells. Bone & Joint Research, 9(10), 667-674.
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5

Culture of Pancreatic Cancer Cell Lines

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Pancreatic cancer cell lines PANC-1 and MIA PaCa-2 and normal cell line (L-929) were purchased from the Korean Cell Bank (Seoul, Republic of Korea). PANC-1 and MIA PaCa-2 cells were cultured with DMEM high glucose containing 10% FBS and 1% penicillin/streptomycin (Biowest, Nuaillé, France). L-929 cells were grown using RPMI 1640 media supplemented with 10% fetal bovine serum (FBS), 2 μM L-glutamine, and 10,000 U/mL of penicillin/streptomycin (Biowest, Nuaillé, France). Cells were incubated at 37 °C and humidified in 5% CO2 conditions (MCO-15AC, Sanyo, Osaka, Japan). Cell subculture was conducted at regular intervals of 2–3 days.
Kim J.W., Choi J., Park M.N, & Kim B. (2023). Apoptotic Effect of Gallic Acid via Regulation of p-p38 and ER Stress in PANC-1 and MIA PaCa-2 Cells Pancreatic Cancer Cells. International Journal of Molecular Sciences, 24(20), 15236.
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6

Culturing 293T and K562 Cell Lines

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293T Cells (CRL11268; American Type Culture Collection; Rockville, MD, USA) were maintained in Dulbelcco’s Modified Eagle’s Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with GlutaMAXTM supplemented with 10% Foetal Bovine Serum (FBS, Biowest, Nuaillé, France) and 1% penicillin/streptomycin (Biowest) at 10% CO2 and 37 °C. The human cell line K562 (lymphoblast from bone marrow chronic myelogenous leukaemia, CCL-243) was obtained from ATCC (Manassas, Virginia, USA), and maintained in RPMI media (Biowest), supplemented with 10% FBS and 1% penicillin/streptomycin at 5% CO2 and 37 °C.
Sánchez-Hernández S., Aguilar-González A., Guijarro-Albaladejo B., Maldonado-Pérez N., Ramos-Hernández I., Cortijo-Gutiérrez M., Sánchez Martín R.M., Benabdellah K, & Martin F. (2020). Development of Cellular Models to Study Efficiency and Safety of Gene Edition by Homologous Directed Recombination Using the CRISPR/Cas9 System. Cells, 9(6), 1492.
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7

Cultivating Vero E6 and HeLa Cells

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Vero E6 cells (CRL-1586, ATCC, Manassas, VA, USA) were cultivated in Dulbecco’s modified eagle’s medium (DMEM, Biowest, Nuaillé, France), 10% fetal calf serum (FCS, c.c.pro, Oberdorla, Germany), 1% sodium pyruvate (Sigma, Steinheim, Germany), 1% non-essential amino acids (NEAA, Biowest) and 1% penicillin/streptomycin (Biowest). For propagation of Hela cells (ACC 57, DSMZ, Braunschweig, Germany), DMEM was supplemented with 10% FCS, 1% NEAA, 1% penicillin/streptomycin and 1% L-Glutamin (Biowest). Both cell lines were incubated at 37 °C and 5% CO2 in a humidified atmosphere.
Tolksdorf B., Nie C., Niemeyer D., Röhrs V., Berg J., Lauster D., Adler J.M., Haag R., Trimpert J., Kaufer B., Drosten C, & Kurreck J. (2021). Inhibition of SARS-CoV-2 Replication by a Small Interfering RNA Targeting the Leader Sequence. Viruses, 13(10), 2030.
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8

Culturing Neuroblastoma and Commonly Used Cell Lines

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SK-N-SH neuroblastoma cells (ATCC® HTB-11™) were cultured under standard conditions (37 °C, 5% CO2) in 90% Minimum Essential Medium (MEM) (Eagle) with Earle’s balanced salt solution (BSS) (Biowest, Nuaillé, France) and Fetal Bovine Serum (FBS) (Thermo Fisher Scientific, Waltham, MA, USA) to a final concentration of 10%, 2 mM l-glutamine, 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, 1 U μg−1 antibiotics (penicillin/streptomycin), and 1.0 mM sodium pyruvate (Biowest, Nuaillé, France). HEK293 (ATCC® CRL-1573™), HeLa (ATCC® CRM-CCL-2™) and MCF-7 (ATCC® HTB-22™) cell lines were grown in Dulbecco’s Modified Eagle Medium (Biochrom Gmbh, Berlin, Germany), supplemented with 10% Fetal Bovine Serum (Thermo Fisher Scientific, Waltham, MA, USA), 2 mM l-glutamine, 0.5 mM sodium pyruvate, and 1% Penicillin-Streptomycin (Biowest, Nuaillé, France) in T-75 flasks (Sarstedt AG & Co. KG, Nümbrecht, Germany). Subcultivation was done in a 1:10 ratio. Cells were detached from culture flasks by treatment with trypsin-EDTA for 3–10 min. After detachment, they were resuspended in the culture medium to inactivate any remaining trypsin activity. After centrifugation for 5 min (1000 rpm), they were resuspended in the medium at concentrations of 106, 2 × 106, and 4 × 106 cells/mL.
Paivana G., Mavrikou S., Kaltsas G, & Kintzios S. (2019). Bioelectrical Analysis of Various Cancer Cell Types Immobilized in 3D Matrix and Cultured in 3D-Printed Well. Biosensors, 9(4), 136.
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9

Cancer Cell Culture Protocols

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H1299 cancer cells were grown in RPMI-1640 medium supplemented with 2 mM L-glutamine, 100 IU/ml penicillin/streptomycin and 10% fetal bovine serum (FBS; Biowest USA). HAP1 and HAP1shmt2KO cells were obtained from Horizon Discovery Ltd., Cambridge, UK and maintained in IMDM medium (Gibco) supplemented with 100 IU/ml penicillin/streptomycin and 10% FBS (Biowest USA). Minimal medium (MEM) formulation and MEM supplementations have been previously reported (21 (link)).
Guiducci G., Paone A., Tramonti A., Giardina G., Rinaldo S., Bouzidi A., Magnifico M.C., Marani M., Menendez J.A., Fatica A., Macone A., Armaos A., Tartaglia G.G., Contestabile R., Paiardini A, & Cutruzzolà F. (2019). The moonlighting RNA-binding activity of cytosolic serine hydroxymethyltransferase contributes to control compartmentalization of serine metabolism. Nucleic Acids Research, 47(8), 4240-4254.
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10

Cell Culture Protocol for Various Cell Lines

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K562 cells were grown in ISCOVE medium (GIBCO, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) (Biowest, Nuaillé, France) and 1X penicillin-streptomycin (Biowest). MCF-7, MCF10A, and HEK-293T cells were cultivated in DMEM (Biowest) medium containing 10% FBS with 10% fetal bovine serum (GIBCO) and 1X penicillin-streptomycin (Biowest). Culture conditions were 37 °C and 5% CO2.
Peralta-Alvarez C.A., Núñez-Martínez H.N., Cerecedo-Castillo Á.J., Poot-Hernández A.C., Tapia-Urzúa G., Garza-Manero S., Guerrero G, & Recillas-Targa F. (2024). A Bidirectional Non-Coding RNA Promoter Mediates Long-Range Gene Expression Regulation. Genes, 15(5), 549.
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