D sucrose

Manufactured by Merck Group

Sourced in United States, Germany, Italy

D-sucrose is a type of sugar commonly used in laboratory settings. It serves as a standard reference material for various analytical and testing procedures. D-sucrose is a white, crystalline solid that is soluble in water and has a sweet taste.

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Lab products found in correlation

D glucose, by Merck Group (18 mentions) D fructose, by Merck Group (14 mentions) D trehalose, by Merck Group (7 mentions) D galactose, by Merck Group (4 mentions) D raffinose pentahydrate, by Merck Group (4 mentions) Ethanol, by Merck Group (3 mentions) Sodium chloride (nacl), by Merck Group (3 mentions) D sorbitol, by Merck Group (3 mentions) Taurine, by Merck Group (3 mentions) Rotenone, by Merck Group (3 mentions) Succinate, by Merck Group (3 mentions) Lactobionic acid, by Merck Group (3 mentions) 2 2 diphenyl 1 picrylhydrazyl (dpph), by Thermo Fisher Scientific (3 mentions) D glucose anhydrous, by Merck Group (2 mentions) Sodium hydroxide (naoh), by Merck Group (2 mentions) D maltose, by Merck Group (2 mentions) Potassium chloride (kcl), by Merck Group (2 mentions) Cacl2 2h2o, by Merck Group (2 mentions) D lactose monohydrate, by Merck Group (2 mentions) Kh2po4, by Merck Group (2 mentions)

Close spelling variants of this product

Sucrose (2008 citations) D-sucrose-13C12 (2 citations)

38 protocols using d sucrose

Spectral Characterization of Saccharide Solutions

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d-(+)-Glucose (or d-glucose, anhydrous, ≥99% purity), d-(−)-fructose (or d-fructose, anhydrous, ≥99% purity), and d-(+)-sucrose (or d-sucrose, anhydrous, ≥99% purity) from the Lach-Ner Group, and α-d-(+)-glucose (or α-d-glucose, anhydrous, 96%) from Sigma-Aldrich were used in this study without further purification. Saccharide powders were dissolved and thoroughly mixed in an appropriate amount of distilled water in volumetric flasks to yield concentrations C of 0.25 mol dm⁻³ ≡ 0.25 M, 0.50 M, and 3.00 M.
Before measuring the spectral responses of d-glucose and d-fructose, their solutions were first left at rest for 24 hours to reach the state of their anomeric equilibrium at a given temperature. Solutions of d-sucrose, which is a non-reducing and therefore mutarotation inactive saccharide, were measured just after 4 hours, only to ensure perfect dissolution. Mutarotation ellipsometric experiments on α-d-glucose were performed right away with freshly prepared solution.

Vala D., Mičica M., Cvejn D, & Postava K. (2023). Broadband Mueller ellipsometer as an all-in-one tool for spectral and temporal analysis of mutarotation kinetics. RSC Advances, 13(10), 6582-6592.

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Culture Media Composition Analysis

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Proteose peptone, yeast extract, tween 80, potassium dihydrogen phosphate, Na acetate, di-ammonium hydrogen-citrate, magnesium sulfate heptahydrate, manganese sulfate monohydrate, disodium oxalate, diammonium oxalate monohydrate, D (+)glucose anhydrous, D (+)-sucrose were purchased from Merck (Darmstadt, Germany). Inulin was purchased from Beneo (Mannheim, Germany). Schaedler (SB) broth was obtained from Merck (Darmstadt, Germany).

Karamad D., Khosravi-Darani K., Hosseini H., Tavasoli S, & Miller A.W. (2019). Evaluation of Oxalobacter formigenes DSM 4420 biodegradation activity for high oxalate media content: An in vitro model. Biocatalysis and agricultural biotechnology, 22, 101378.

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Synthesis and Characterization of Hyaluronic Acid-Phospholipid Conjugates

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All the phospholipids, cholesterol (CHOL), PLGA 50:50 (Resomer^® RG 502 H, 7–17 kDa), PLGA 75:25 (Resomer^® RG 752 H, 4–15 kDa), PEG₂₀₀₀-PLGA 50:50 (PLGA 11.5 kDa), D-(+)-glucose, D-(+)-sucrose, D-(+)-trehalose, D-(+)-mannitol (purity minimum 95%) and solvents (analytical grade) were purchased from Merck (Milan, Italy). Sodium hyaluronate (HA) (4.8 kDa or 14.8 kDa) was purchased from Lifecore Biomedical (Chaska, MN, USA). The compound 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) was conjugated to 4.8 kDa or 14.8 kDa HA (HA_4.8-DPPE or HA_14.8-DPPE) using the method described by Arpicco et al. [30 (link)]. Filtered MilliQ^® water (Millipore, Merck) was used. Solvents were evaporated using a rotating evaporator (Heidolph Laborota 400, Heidolph Instruments, Schwabach, Germany) equipped with a vacuum pump (Diaphragm Vacuum Pump DC-4).

Andreana I., Bincoletto V., Manzoli M., Rodà F., Giarraputo V., Milla P., Arpicco S, & Stella B. (2023). Freeze Drying of Polymer Nanoparticles and Liposomes Exploiting Different Saccharide-Based Approaches. Materials, 16(3), 1212.

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Preparation of Aqueous Solutions

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Deionized water (DIW) purified by a Milli-Q 50 system (TKA, Germany) was employed in all experiments. Acetone, sodium hydroxide (NaOH), potassium phosphate dibasic (K

_{2}

HPO

_{4}

), sodium chloride (NaCl), sulfuric acid (

97 %

_{2}

_{4}

), D(+)-sucrose, ethanol, and aniline (

99 %

_{6}

_{5}

_{2}

) were bought from Merck. The nitrogen and oxygen gas

6.0

purity bottles were provided by Messer. The multimode optical fiber of 400 μm diameter was purchased from Thorlabs. The Pasteur glass pipettes, the double wall glass, and the magnetic stir plate were supplied by VWR.

Antohe I., Jinga L.I., Antohe V.A, & Socol G. (2021). Sensitive pH Monitoring Using a Polyaniline-Functionalized Fiber Optic—Surface Plasmon Resonance Detector. Sensors (Basel, Switzerland), 21(12), 4218.

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Silylation of Carbohydrates and Inositols

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For this study, yo-inositol (≥99%), scyllo-inositol (≥98%), D-(-)-sorbitol (≥99%), D-(+)-sucrose (≥99.5%), D-(+)-lactose (≥99.5%), D-(+)-maltose (≥99%), isomaltose (≥98%) xylitol (≥99%), and ethanol (≥99.8%) were purchased from Merck (Darmstadt, Germany). The silylating reagents including a mixture of hexamethyldisilazane (HMDS)/chlorotrimethylsilane (TMCS)/pyridine (2:1:10, v/v/v) and a mixture of N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) with 10% (TMCS) trimethylchlorosilane and pyridine (≥99.8%) were obtained from Sigma-Aldrich (Milan, Italy).

Paolini M., Perini M., Allari L., Tonidandel L., Finato F., Guardini K, & Larcher R. (2023). Myo-Inositol, Scyllo-Inositol, and Other Minor Carbohydrates as Authenticity Markers for the Control of Italian Bulk, Concentrate, and Rectified Grape Must. Molecules, 28(8), 3609.

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Saponins and 6-OHDA Assay Protocol

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The saponins, 3 HEPES (N-[2-Hydroxyethyl]piperazine-Nʹ-[2-ethanesulfonic acid]) as well as other chemicals, trichloroacetic acid (TCA), percoll, 6-hydroxydopamine (6-OHDA) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), α-glucosidase from Saccharomyces cerevisiae were obtained from Sigma Aldrich. NaCl, KCl, D-glucose, CaCl 2 ×2H 2 O, 2,2ʹ-dinitro-5,5ʹ-dithiodibenzoic acid (DTNB), D-sucrose, MgCl 2 ×6H 2 O, NaH 2 PO 4 were purchased from Merck. Acarbose and p-nitrophenyl-α-D-glucopyranoside were obtained from Alfa Aesar.
UHPLC-HRMS analysis was performed using a Thermo Scientific Dionex Ultimate 3000 RSLC (Germering, Germany) consisting of 6-channel degasser SRD-3600, high-pressure gradient pump HPG-3400RS, autosampler WPS-3000TRS, and column compartment TCC-3000RS coupled to Thermo Scientific Q Exactive Plus (Bremen, Germany) mass spectrometer.

Kokanova-Nedialkova Z., Kondeva-Burdina M., & Nedialkov P. (2021). Saponins from the roots of Chenopodium bonus-henricus L. with neuroprotective and anti-α-glucosidase activities. Pharmacia, 68(2), 387-392.

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Carbohydrate Profiling: Standards and Preparation

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Standards of mono- and disaccharides: d-fructose, d-glucose, d-galactose, d-(+)-sucrose, d-lactose monohydrate, and ammonia solution (25%, LC-MS LiChropur™ grade) were obtained from Sigma-Aldrich (Darmstadt, Germany). Glucose-¹³C₆ (Glu-¹³C₆, U-¹³C₆, 99%, chemical purity 98%) and lactose monohydrate (Lac-¹³C₆, UL-¹³C₆glc, 98%+) were procured from Cambridge Isotope Laboratories Inc. (Tewksbury, MA, USA). Ultrapure water (18.2 MΩ.cm) was prepared with MilliQ^® Direct-Q^® UV (Merck KGaA, Darmstadt, Germany). Acetonitrile (MeCN; LiChrosolv, HPLC gradient grade), and guanidine hydrochloride (GuHCl; ≥99%) were acquired from Sigma-Aldrich (Darmstadt, Germany). Biotage Isolute® PLD+, C18 and NH₂ were procured from Biotage Sweden AB (Uppsala, Sweden). Amicon Ultra-0.5 centrifugal filter unit (3 kDa) and Millex-LCR filters (Pore size 0.2 µm, Filter Dimension 13 mm) were obtained from Merck KGaA (Darmstadt, Germany) and Microsep Advance Centrifugal Devices with Omega Membrane 1K filter unit was purchased from Pall Corporation (Port Washington, NY, USA).

Pismennõi D., Kiritsenko V., Marhivka J., Kütt M.L, & Vilu R. (2021). Development and Optimisation of HILIC-LC-MS Method for Determination of Carbohydrates in Fermentation Samples. Molecules, 26(12), 3669.

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Cerebral Vessel Respiration Measurement

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The cerebral vessels were placed in ice‐cold respiration medium consisting of 0.5 mmol/L EGTA (Sigma, catalog No. E4378), 3 mmol/L MgCl₂ 6 hexahydrate (Sigma, catalog No. M9272), 60 mmol/L lactobionic acid (Sigma, catalog No. 153516), 20 mmol/L taurine (Sigma, catalog No. T0625), 10 mmol/L KH₂PO₄ (Sigma, catalog No. P5655), 20 mmol/L HEPES (ThermoFisher Scientific, catalog No. 15630080), 110 mmol/L D‐sucrose (Sigma, catalog No. S0389), and 1 g/L BSA at pH 7.1.²⁷ High‐resolution oxygen consumption measurements were conducted in 2 mL of MiR05 using the Oroboros Oxygraph 2k (Oroboros Instruments, Innsbruck, Austria). Polarographic oxygen measurements were acquired at 2‐second intervals with the steady‐state rate of respiration calculated from a minimum of 30 data points and expressed as pmol s^‐1 per mg wet weight. All respiration measurements were conducted at 37°C in a working range [O2] of ≈200 to 100 μmol/L. Respiration was measured with sequential titrations of: 1.25 mmol/L ADP (CalBiochem, catalog No. 117105), 2 mmol/L malate (Sigma, catalog No. M1000), 5 mmol/L pyruvate (Sigma, catalog No. P2256), 10 mmol/L glutamate (Sigma, catalog No. G1626), 10 mmol/L succinate (Sigma, catalog No. S2378), 0.5 μmol/L carbonyl cyanide m‐chlorophenyl hydrazone (Sigma, catalog No. C2759), 0.5 μmol/L rotenone (Sigma, catalog No. R8875), and 2.5 μmol/L antimycin‐A (Sigma, catalog No. A8674).

Tyrrell D.J., Blin M.G., Song J., Wood S.C, & Goldstein D.R. (2020). Aging Impairs Mitochondrial Function and Mitophagy and Elevates Interleukin 6 Within the Cerebral Vasculature. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 9(23), e017820.

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Fabrication of Porous Polymer Bridges

Cited 2 times

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Bridges were fabricated using a sacrificial template variation(J. Li, T. A. Rickett, & R. Shi, 2009 (link)) of the gas foaming/particulate leaching technique, as previously described(A. Thomas et al., 2013 (link); H. M. Tuinstra et al., 2012 (link)). Briefly, PLG (75:25 lactide:glycolide; i.v. 0.76 dL/g; Lakeshore Biomaterials, Birmingham, AL, USA) was dissolved in dichloromethane (6% w/w) and emulsified in 1% poly (ethylene-alt-maleic anhydride) using a homogenizer (PolyTron 3100; Kinematica AG, Littau, Switzerland) to create microspheres (z-average diameter ~1μm). D-sucrose (Sigma Aldrich), D-glucose (Sigma Aldrich), and dextran MW 100,000 (Sigma Aldrich) were mixed at a ratio of 5.3:2.5:1 respectively by mass. The mixture was caramelized, cooled, and drawn from solution with a Pasteur pipette to make sugar fibers. Fibers were drawn to 150 – 250 μm, coated with a 1:1 mixture of PLG microspheres and salt (63–106 μm) and pressed into a salt-lined aluminum mold. The sugar strands were used to create 7 channels and the salt create a porous structure. The materials were then equilibrated with CO₂ gas (800 psi) for 16 h and then gas foamed in a custom-made pressure vessel. Bridges were subsequently cut into 2.25 mm sections and leached for 2 h to remove porogen. The bridges are dried overnight and stored in a desiccator.

Smith D.R., Margul D.J., Dumont C.M., Carlson M.A., Munsell M.K., Johnson M., Cummings B.J., Anderson A.J, & Shea L.D. (2018). Combinatorial lentiviral gene delivery of pro-oligodendrogenic factors to improve myelination of regenerating axons after spinal cord injury. Biotechnology and bioengineering, 116(1), 155-167.

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Quantification of Carbohydrates in Flour

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Flour (2.5 g) was dispersed in 25 ml Milli-Q/ethanol mixture (50–50% (v/v)) and centrifuged (10 min, 3000 rpm). The supernatant was filtered (RC HPLC filter, RC Minisart Satorius, Germany) and centrifuged (15 min, 14,000 rpm). A calibration curve was made for D-fructose (Merck) and D-sucrose (Sigma Aldrich). Quantification of carbohydrates (sucrose and fructose) was by HPLC using an Evaporative Light Scattering Detector (ELSD) [25 (link)] and the Alltech prevail Carbohydrates ES 5u 250*4.60 mm column. Flow rate was 0.8 ml/min for 35 min using 100% acetonitrile and deionised water as eluents.

Mubaiwa J., Fogliano V., Chidewe C, & Linnemann A.R. (2018). Bambara groundnut (Vigna subterranea (L.) Verdc.) flour: A functional ingredient to favour the use of an unexploited sustainable protein source. PLoS ONE, 13(10), e0205776.

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