Wistar rats injected with NTS were randomly divided into six groups: Group 1 (n = 5), receiving daily intraperitoneal injections of methylprednisolone (2.5 μg/g; Pfizer) on day 1 (24 h after the injection of NTS) through 7; Group 2 (n = 5), daily treatment of methylprednisolone (2.5 μg/g; Pfizer) and RU486 (20 μg/g; Sigma-Aldrich) on day 1 through 7; Group 3 (n = 5), daily treatment of saline intraperitoneally as control; Group 4 (n = 5), daily intraperitoneal injections of methylprednisolone (2.5 μg/g; Pfizer) on day 7 through 13; Group 5 (n = 5), daily treatment of methylprednisolone (2.5 μg/g; Pfizer) and RU486 (20 μg/g; Sigma-Aldrich) on day 7 through 13; and Group 6 (n = 5), daily treatment of saline intraperitoneally as control. Twenty-four hour after the final treatment, all rats were sacrificed and serum samples collected. Kidney samples were processed for histological analysis, preparation of protein, total RNA, and glomeruli. methylprednisolone is a systemic glucocorticoid that is used to treat patients with glomerulonephritis and rats of NTS model in literature.
Methylprednisolone
Manufactured by Pfizer
Sourced in United States, Belgium, Italy, China
Methylprednisolone is a synthetic corticosteroid medication used as an anti-inflammatory and immunosuppressant drug. It is primarily used in the treatment of various inflammatory and autoimmune conditions.
Automatically generated - may contain errors
Lab products found in correlation
Lipopolysaccharides (lps), by Merck Group (6 mentions)
Lipopolysaccharide, by Merck Group (4 mentions)
Mycophenolate, by Genentech (3 mentions)
Lipopolysaccharides (lps), by Pfizer (3 mentions)
Sprague dawley rat, by Charles River Laboratories (1 mentions)
C57bl 6 mice, by GemPharmatech (1 mentions)
Anti e cadherin antibody, by BD (1 mentions)
Mtser1 medium, by STEMCELL (1 mentions)
Y 27632, by STEMCELL (1 mentions)
Chir99021, by Cayman Chemical (1 mentions)
Advanced rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Accutase, by STEMCELL (1 mentions)
Sodium heparin, by Fresenius (1 mentions)
Everolimus, by Selleck Chemicals (1 mentions)
Baricitinib, by Selleck Chemicals (1 mentions)
Tofacitinib, by Selleck Chemicals (1 mentions)
Dexamethasone (dex), by Selleck Chemicals (1 mentions)
Sirolimus, by Selleck Chemicals (1 mentions)
Dasatinib, by Selleck Chemicals (1 mentions)
Temsirolimus, by Selleck Chemicals (1 mentions)
40 protocols using methylprednisolone
1
Glucocorticoid Therapy in Glomerulonephritis
2
Osteonecrosis Induction and BADGE Treatment in Rabbits
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After approval from the Animal Ethical Committee of Xi’an Jiaotong University, 36 28-week-old male New Zealand white rabbits (4 ± 0.5 kg) from the Experimental Animal Center of Xi’an Jiaotong University, China were included in this study. All experimental animals were housed under standard conditions.
Rabbits were randomized into 3 groups: the normal group (N = 12), the model group (N = 12) and the BADGE group (N = 12). Osteonecrosis of femurs was induced using methods presented in the previously published protocols [16 (link)]. Briefly, one injection of 10 μg/kg body weight of lipopolysaccharide (Sigma, St. Louis, MO, USA) was administered intravenously. Twenty-four hours later, three injections of 20 mg/kg body weight of methylprednisolone (Pfizer, USA) were administered intramuscularly, at a time interval of 24 h. Osteonecrosis gradually developed in femurs 6 weeks after injection of methylprednisolone. In the model group, the rabbits were treated with vehicle. In the BADGE group, the rabbits received treatment for a total of 6 weeks with BADGE(daily intra-peritoneal injection of 10 mg/kg in 15% DMSO). The doses and durations of treatment of BADGE were determined based on a previously published study [15 (link)]. Body weight of the rabbits were measured each week.
Rabbits were randomized into 3 groups: the normal group (N = 12), the model group (N = 12) and the BADGE group (N = 12). Osteonecrosis of femurs was induced using methods presented in the previously published protocols [16 (link)]. Briefly, one injection of 10 μg/kg body weight of lipopolysaccharide (Sigma, St. Louis, MO, USA) was administered intravenously. Twenty-four hours later, three injections of 20 mg/kg body weight of methylprednisolone (Pfizer, USA) were administered intramuscularly, at a time interval of 24 h. Osteonecrosis gradually developed in femurs 6 weeks after injection of methylprednisolone. In the model group, the rabbits were treated with vehicle. In the BADGE group, the rabbits received treatment for a total of 6 weeks with BADGE(daily intra-peritoneal injection of 10 mg/kg in 15% DMSO). The doses and durations of treatment of BADGE were determined based on a previously published study [15 (link)]. Body weight of the rabbits were measured each week.
3
Methylprednisolone Treatment in EAE Rat Model
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Twenty-six rats were randomly divided into three groups: the early treatment group (n = 13), the late treatment group (n = 7), and the control group (n = 6). EAE models were induced in all the rats as described before. According to the results of the EAE rat models for early diagnosis as above, the early treatment group received intraperitoneal injection of methylprednisolone (Pfizer) on the 9th day after modelling, and the late treatment group received intraperitoneal injection of methylprednisolone after disease progression (day 10, 11 and 12). methylprednisolone was injected at a dose of 30 mg kg−1 for 5 consecutive days. The control group was injected with the same amount of normal saline after onset. Body weight and neurological impairment scores were evaluated daily as mentioned above. Statistical analysis was performed with GraphPad Prism 9.0.1 (GraphPad, San Diego, CA, USA). Data were tested for statistical significance using the paired Student's t-test.
4
Xenogeneic Lung Transplant Immunosuppression
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To minimize the risk of immunologic rejection, an immunosuppression regimen informed by established protocols and current practices in clinical lung transplantation was used (Fig. 1B ). Four hours before initiation of xenogeneic XC, xeno-support swine were anesthetized, intubated, and administered CVF (1 mg; Sigma-Aldrich) to deplete complement activity (61 (link), 62 (link)). Intravenous diphenhydramine (50 mg; West-Ward Pharmaceuticals) and methylprednisolone (1 g; Pfizer) were administered to mitigate hemodynamic instability occasionally associated with CVF. Intravenous tacrolimus (5 mg; Astellas) and mycophenolate (500 mg; Genentech) were also administered before reperfusion and redosed every 12 hours. methylprednisolone (125 mg; Pfizer) was readministered every 8 hours after the initial dose.
5
Xenogeneic Lung Transplant Immunosuppression
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To minimize the risk of rejection, an immunosuppression regimen informed by current practices in clinical transplantation, and described for use in xeno-support of human lungs, was used (Figure 1 B).14 ,15 (link) At 4 hours before cross-circulation, xeno-support swine were anesthetized, intubated, and administered cobra venom factor (1 mg; Sigma-Aldrich) to deplete complement activity.16 (link) Intravenous diphenhydramine (50 mg; West Ward) and methylprednisolone (1 g; Pfizer) were administered to limit the inflammatory response associated with cobra venom factor. Intravenous tacrolimus (5 mg; Astellas) and mycophenolate (500 mg; Genentech) were also administered before reperfusion and redosed every 12 hours (Supplemental Figure S1C, http://links.lww.com/HEP/F706 ). methylprednisolone (125 mg; Pfizer) was readministered every 8 hours after the initial dosage.
6
Immunosuppressive Regimen for Xenotransplantation
7
Rat Model of Osteonecrosis Treated with BMSCs and miR-27a
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The Animal Research Committee of the Fifth Affiliated Hospital of Xinjiang Medicine University approved all procedures carried out (IACUC20170730-2). All methods are reported in accordance with ARRIVE guidelines. All methods were carried out in accordance with relevant guidelines and regulations. A total of 16 Sprague–Dawley (SD) rats were obtained from the Fifth Affiliated Hospital of Xinjiang Medicine University. They were equally randomized into 4 groups: the control group (Ctrl), ONFH group, BMSCs treated ONFH group, BMSCs and miR-27a mimic treated ONFH group. For establishment of the ONFH model, the rats were injected intraperitoneally with 20 μg/kg lipopolysaccharide (LPS; Sigma, CA, USA). Then, 40 mg/kg methylprednisolone (Pfizer Inc., Ascoli Piceno, Italy) were injected intramuscularly for three times at intervals of 24 h. The rats in the control group were administered normal saline with the same volume. For BMSCs treated ONFH group or BMSCs and miR-27a mimic treated ONFH group, the bone marrow cavity of rat femur was unilaterally injected with approximately 106 BMSCs or 106 BMSCs transfected with miR-27a mimics. After four weeks, all rats were sacrificed with anesthetizing using sevoflurane and then bone marrow tissue and serum were collected.
8
Therapeutic Effects of hUC-MSCs in Rat Sepsis Model
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The animal study protocol was approved by the Wuhan University Ethics Committee (number: 20180920). 30 male Sprague-Dawley rats with approximate weights of 360 ± 26 g, that were 28 weeks old were purchased from Beijing Vital River Laboratory Animal Technology Co. Ltd. The rats were randomly divided into three groups of control group, model group, and treatment group. The rats in the control group received only 0.9% of the tail vein saline injection within one week. The rats in the model group were administered with 2 mg/kg of lipopolysaccharide (Sigma-Aldrich, USA) intravenously once daily for two days and injected with 20 mg/kg of methylprednisolone (Pfizer, USA) intramuscularly once daily for five days. The procedure for the treatment group was similar to that of the model group, apart from the fact that 1 × 106 hUC-MSCs (Shenzhen Wingor Bio-technology Co., Ltd., China) were injected into the tail vein 1 h following the intramuscular injection of methylprednisolone. The femoral head samples and venous blood of the rats were removed under anesthesia after 28 days of rearing and then used for further analysis.
9
Evaluation of Traditional Chinese Medicine Compounds in Inflammation
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The drug of QYSXC was produced by Shaanxi academy of traditional Chinese medicine (lot number: 20181213). Xianlinggubao Capsule (XLGBC) was purchased from Sinopharm Group Tongjitang (Guizhou) Pharmaceutical Co., Ltd. (lot number: 180503). LPS was purchased from Sigma-Aldrich (St. Louis, MO, USA). Methylprednisolone was purchased from Pfizer (New York, NY, USA).
10
BMSC Methylprednisolone Model of SNFH
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Human BMSC progeny were procured from Procell (Wuhan, China), cultivated, and propagated to the third generation. Subsequently, human BMSCs were subjected to methylprednisolone treatment (Pfizer, USA) to establish a model of SNFH BMSCs.
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