B 1355

Manufactured by Vector Laboratories

Sourced in Japan, United States

B-1355 is a laboratory equipment product offered by Vector Laboratories. It is designed to perform a core function, but a detailed and unbiased description is not available at this time.

Automatically generated - may contain errors

Lab products found in correlation

Vectashield medium h 1400, by Vector Laboratories (5 mentions) Alexa fluor 594 conjugated streptavidin, by Thermo Fisher Scientific (5 mentions) Ab1031, by Merck Group (3 mentions) Mab1581, by Merck Group (3 mentions) Ab7260, by Abcam (2 mentions) Mab5406, by Merck Group (2 mentions) Ab5905, by Merck Group (2 mentions) Alexa fluor 594 conjugated goat anti guinea pig, by Thermo Fisher Scientific (2 mentions) Fitc conjugated anti mouse igm, by Santa Cruz Biotechnology (2 mentions) Texas red conjugated goat anti rabbit ti 1000, by Vector Laboratories (2 mentions) Fitc conjugated anti mouse igm sc 2082, by Santa Cruz Biotechnology (2 mentions) Alexa fluor 488 conjugated goat anti mouse igg, by Abcam (2 mentions) Alexa fluor 594 conjugated streptavidin s11227, by Thermo Fisher Scientific (2 mentions) Texas red conjugated goat anti rabbit, by Vector Laboratories (2 mentions) Ab150113, by Abcam (1 mentions) Dylight 488 streptavidin, by Vector Laboratories (1 mentions) H 1400, by Vector Laboratories (1 mentions) Vectashield medium, by Vector Laboratories (1 mentions) Vectashield mounting medium, by Vector Laboratories (1 mentions) Mab377, by Merck Group (1 mentions)

16 protocols using b 1355

Immunohistochemical Staining Protocols

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We used the following lectins and primary antibodies for staining: biotinylated WFA (B-1355, Vector Laboratories; 1 : 200), mouse anti-parvalbumin (clone PARV-19, P3088; Sigma-Aldrich Japan, Tokyo, Japan; 1 : 1,000), mouse anti-NeuN (clone A60, MAB377; Millipore, Bedford, MA; 1 : 500), mouse anti-aggrecan (Cat-315; MAB1581, Millipore; 1 : 1,000), rabbit anti-GFAP (ab7260; Abcam, Cambridge, MA; 1 : 1,000), rabbit anti-IBA-1 (019-19741; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan; 1 : 1,000), mouse anti-GAD67 (clone 1G10.2, MAB5406; Millipore; 1 : 1,000), and guinea pig anti-VGLUT1 (AB5905; Millipore; 1 : 1,000).
We used the following secondary antibodies for visualization: Alexa Fluor 488-conjugated goat anti-mouse IgG (ab150113; Abcam, Cambridge, MA; 1 : 1,000), Alexa Fluor 594-conjugated goat anti-guinea pig (A-11076; Thermo Fisher Scientific, Waltham, MA; 1 : 500), FITC-conjugated anti-mouse IgM (sc-2082, Santa Cruz Biotechnology, Santa Cruz, CA, 1 : 1,000), Texas Red-conjugated goat anti-rabbit (TI-1000; Vector Laboratories, Funakoshi Co., Tokyo, Japan), and streptavidin-conjugated Alexa Fluor 594 (S11227, Thermo Fisher Scientific; 1 : 1,000).

Ueno H., Suemitsu S., Murakami S., Kitamura N., Wani K., Takahashi Y., Matsumoto Y., Okamoto M, & Ishihara T. (2019). Alteration of Extracellular Matrix Molecules and Perineuronal Nets in the Hippocampus of Pentylenetetrazol-Kindled Mice. Neural Plasticity, 2019, 8924634.

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Immunostaining of Wisteria floribunda Lectin

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The sections were treated with 0.1% Triton X-100 and PBS at room temperature for 15 min. After three washes with PBS, sections were incubated with 10% normal goat serum (ImmunoBioScience Corp., Mukilteo, WA, USA) in PBS at room temperature for 1 h. They were then washed thrice with PBS and incubated overnight at 4 °C in PBS containing biotinylated Wisteria floribunda agglutinin (WFA) (B-1355, Vector Laboratories; 1:200) and/or antibodies described in the subsequent text. After washing with PBS, the sections were incubated with Alexa Fluor 594-conjugated streptavidin (S11227; Molecular Probes, Eugene, OR) and/or the corresponding secondary antibodies (described in the subsection Antibodies and lectins) at room temperature for 2 h. The labeled sections were then rinsed with PBS and mounted on glass slides using Vectashield medium (H-1400; Vector Laboratories, Funakoshi Co., Tokyo, Japan). The prepared slides were stored at 4 °C until microscopic analysis was performed.

Ueno H., Takahashi Y., Murakami S., Wani K., Matsumoto Y., Okamoto M, & Ishihara T. (2022). Fingolimod increases parvalbumin-positive neurons in adult mice. IBRO Neuroscience Reports, 13, 96-106.

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Comprehensive Immunohistochemical Staining Protocol

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The following lectins and primary antibodies were used for staining: biotinylated WFA (B-1355, Vector Laboratories; 1:200), mouse anti-parvalbumin (clone PARV-19, P3088; Sigma-Aldrich Japan, Tokyo, Japan; 1:1000), mouse anti-aggrecan (Cat-315; MAB1581, MERCK; 1:1000), rabbit anti-aggrecan (AB1031, Millipore, Tokyo, Japan; 1:200), rabbit anti-glial fibrillary acidic protein (GFAP) (ab7260; Abcam, Cambridge, MA; 1:1000), rabbit anti-ionized calcium-binding adapter molecule 1 (Iba-1) (019–19741; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan; 1:1000), mouse anti-NeuN (clone A60, MAB377; Millipore; 1:500), and mouse anti-glutamate decarboxylase (GAD67) (clone 1G10.2, MAB5406; Millipore, Bedford, MA; 1:1000).
The following secondary antibodies were used for visualization: Alexa Fluor 488-conjugated goat anti-mouse IgG (ab150113; Abcam, Cambridge, MA; 1:1000), FITC-conjugated anti-mouse IgM (sc-2082, Santa Cruz Biotechnology, Santa Cruz, CA, 1:1000), Texas Red-conjugated goat anti-rabbit (TI-1000; Vector Laboratories, Funakoshi Co., Tokyo, Japan), and streptavidin-conjugated Alexa Fluor 594 (S11227, Thermo Fisher Scientific; 1:1000).

Ueno H., Takahashi Y., Murakami S., Wani K., Matsumoto Y., Okamoto M, & Ishihara T. (2022). Fingolimod increases parvalbumin-positive neurons in adult mice. IBRO Neuroscience Reports, 13, 96-106.

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Co-Staining of MMP13 and PNN in Rat Brains

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Co-staining for MMP13 and PNN was done as described in (Rankin-Gee et al., 2015 (link)) with slight modifications. Rats were perfused with 4% paraformaldehyde via cardiac infusion. Brains were removed and kept in 4% paraformaldehyde overnight before being transferred to a 30% sucrose solution. Forty micrometer brain slices were collected using a microtome and were stored in cryopreservative. Slices were washed with PBS five times for 5 min each. Slices were blocked in PBS + 2% BSA for 1 h at room temperature. Sections were co-incubated in biotinylated Wisteria Floribunda Lectin (WFA) (20 μg/ml, B-1355, Vector Labs) and anti-MMP13 antibody (1:100, MAB13426, EMD Millipore Corp., CA) in PBS + 2% BSA overnight. Sections were incubated in DyLight 488 Streptavidin (1:500, SA-5488–1, Vector Labs) and 594 Alexa fluorescent-conjugated goat anti-mouse antibody for 3 h at room temperature after washes in PBS. Slices were then mounted with Vectashield mounting medium after staining with DAPI. Confocal images of stained sections were captured using Leica Microsystems, IL, USA with a 40X magnification oil lens. Images were processed in ImageJ.

Dubey D., McRae P.A., Rankin-Gee E.K., Baranov E., Wandrey L., Rogers S, & Porter B.E. (2017). Increased metalloproteinase activity in the hippocampus following status epilepticus. Epilepsy research, 132, 50-58.

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Immunostaining of Cryostat Sections

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We treated the cryostat sections with 0.1% Triton X-100 with PBS at room temperature for 15 min. After three washes with PBS, we incubated the sections with 10% normal goat serum (ImmunoBioScience Corp., Mukilteo, WA) in PBS at room temperature for 1 h, we washed them three times with PBS, and we incubated them overnight at 4°C in PBS containing biotinylated WFA (B-1355, Vector Laboratories; 1 : 200) and/or the antibodies described in Antibodies and Lectins. After washing with PBS, we incubated the sections with Alexa Fluor 594-conjugated streptavidin (S11227; Molecular Probes, Eugene, OR) and/or the corresponding secondary antibodies (described in Antibodies and Lectins) at room temperature for 2 h. We rinsed the labeled sections again with PBS, and we mounted them on glass slides with VECTASHIELD medium (H-1400; Vector Laboratories, Funakoshi Co., Tokyo, Japan). We stored the prepared slides at 4°C until we used them in the microscopy analysis.

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Cryostat Sectioning and Labeling

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The cryostat sections were treated with 0.1% triton X-100 in PBS for 15 min at 20 °C. After three washes in PBS, the sections were incubated with 10% normal donkey serum (ImmunoBioScience Co., WA) in PBS for 1 h at 20 °C. Sections were again washed three times in PBS and incubated with biotinylated WFA (B-1355, Vector Laboratories, Funakoshi Co., Tokyo, Japan; 1:200) and a primary antibodies in PBS overnight at 4 °C. After washing in PBS, the sections were incubated with streptavidin-conjugated to Alexa Fluor 594 (S11227, Thermo Fisher Scientific, Tokyo, Japan; 1:1000) and secondary antibodies in PBS at 20 °C for 2 h. The sections were rinsed with PBS and mounted onto glass slides using Vectashield mounting medium (H-1400, Vector Laboratories). The prepared slides were stored at 4 °C until imaging.

Ueno H., Suemitsu S., Murakami S., Kitamura N., Wani K., Matsumoto Y., Okamoto M., Aoki S, & Ishihara T. (2018). Juvenile stress induces behavioral change and affects perineuronal net formation in juvenile mice. BMC Neuroscience, 19, 41.

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Immunohistochemical Labeling of Cryostat Sections

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We treated the cryostat sections with 0.1 % Triton X-100 with PBS at room temperature for 15 min. After three washes with PBS, we incubated the sections with 10 % normal goat serum (ImmunoBioScience Corp., Mukilteo, WA) in PBS at room temperature for 1 h. Sections were then washed three times with PBS, and incubated overnight at 4 °C in PBS containing biotinylated WFA (B-1355, Vector Laboratories; 1:200) and/or the antibodies described in the subsection “Antibodies and lectins.” After washing with PBS, we incubated the sections with Alexa Fluor 594-conjugated streptavidin (S11227; Molecular Probes, Eugene, OR) and/or the corresponding secondary antibodies (described in the subsection “Antibodies and lectins”) at room temperature for 2 h. We rinsed the labeled sections again with PBS and mounted them on glass slides with Vectashield medium (H-1400; Vector Laboratories, Funakoshi Co., Tokyo, Japan). We stored the prepared slides at 4 °C until microscopy analysis was performed.

Ueno H., Shimada A., Suemitsu S., Murakami S., Kitamura N., Wani K., Takahashi Y., Matsumoto Y., Okamoto M, & Ishihara T. (2020). Alpha-pinene and dizocilpine (MK-801) attenuate kindling development and astrocytosis in an experimental mouse model of epilepsy. IBRO Reports, 9, 102-114.

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Immunostaining of Extracellular Matrix Components

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The sections were then treated with 0.1% Triton X-100 and PBS at room temperature for 15 min. After three washes with PBS, sections were incubated with 10% normal goat serum (ImmunoBioScience Corp., Mukilteo, WA, USA) in PBS at room temperature for 1 h. They were then washed thrice with PBS and incubated overnight at 4 °C in PBS containing biotinylated WFA (B-1355, Vector Laboratories; 1:200) and the antibodies described in the subsequent text. Subsequently, the sections were incubated with Alexa Fluor 594-conjugated streptavidin (S11227; Molecular Probes, Eugene, OR, USA) and the corresponding secondary antibodies (described in the Lectins and antibodies subsection) at room temperature for 2 h. The labeled sections were then rinsed with PBS and mounted on glass slides using a Vectashield medium (H-1400; Vector Laboratories, Funakoshi Co., Tokyo, Japan). The prepared slides were stored at 4 °C until further microscopic analyses.

Ueno H., Takahashi Y., Murakami S., Wani K., Miyazaki T., Matsumoto Y., Okamoto M, & Ishihara T. (2023). Component-specific reduction in perineuronal nets in senescence-accelerated mouse strains. IBRO Neuroscience Reports, 14, 111-121.

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Wisteria floribunda Lectin Binds Chondroitin Sulfate

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WFA (catalog #B-1355, Vector Labs), a lectin isolated from seeds of Wisteria floribunda, binds specifically to N-acetyl-D-galactosamine on the terminal end of chondroitin sulfate (CS) chains, with a preference for β glycosidic linkage (Kurokawa et al., 1976 (link)). The specificity of WFA as a marker for these macromolecules is supported by extensive literature, including ablation of labeling following CS enzymatic digestion (Galtrey and Fawcett, 2007 (link); Pantazopoulos et al., 2010 (link)).

Pantazopoulos H., Gisabella B., Rexrode L., Benefield D., Yildiz E., Seltzer P., Valeri J., Chelini G., Reich A., Ardelt M, & Berretta S. (2020). Circadian Rhythms of Perineuronal Net Composition. eNeuro, 7(4), ENEURO.0034-19.2020.

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Comprehensive Immunohistochemical Staining Protocol

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We used the following lectins and primary antibodies for staining: biotinylated WFA (B-1355, Vector Laboratories; 1:200), mouse anti-parvalbumin (PV; clone PARV-19, P3088; Sigma-Aldrich Japan, Tokyo, Japan; 1:1000), mouse anti-NeuN (clone A60, MAB377; Millipore; 1:500), mouse anti-aggrecan (Cat-315; MAB1581, MERCK; 1:1000), rabbit anti-glial fibrillary acidic protein (GFAP; ab7260; Abcam, Cambridge, MA; 1:1000), rabbit anti-ionized calcium-binding adapter molecule 1 (Iba-1; 019–19741; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan; 1:1000), mouse anti-glutamic acid decarboxylase 67 (GAD67; clone 1 G10.2, MAB5406; Millipore, Bedford, MA; 1:1000), and guinea pig anti-vesicular glutamate transporter 1 (VGLUT1; AB5905; Millipore; 1:1000).
We used the following secondary antibodies for visualization: Alexa Fluor 488-conjugated goat anti-mouse IgG (ab150113; Abcam, Cambridge, MA; 1:1000), Alexa Fluor 594-conjugated goat anti-guinea pig (A-11076; Thermo Fisher Scientific, Waltham, MA; 1:500), FITC-conjugated anti-mouse IgM (sc-2082, Santa Cruz Biotechnology, Santa Cruz, CA, 1:1000), Texas Red-conjugated goat anti-rabbit (TI-1000; Vector Laboratories, Funakoshi Co., Tokyo, Japan), and streptavidin-conjugated Alexa Fluor 594 (S11227, Thermo Fisher Scientific; 1:1000).

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