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Monoclonal murine anti polyhistidine antibody

Manufactured by Merck Group
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Monoclonal murine anti‐polyhistidine antibody is a laboratory reagent used to detect and purify proteins tagged with a polyhistidine sequence. It binds specifically to the polyhistidine tag, allowing for the identification and isolation of the tagged protein.

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Lab products found in correlation

Sigmafast bcip nbt, by LI COR (2 mentions) Signalfire elite ecl reagent, by LI COR (2 mentions) C digit chemiluminescent blot scanner, by LI COR (2 mentions) Phospho threonine antibody, by Cell Signaling Technology (2 mentions) Mouse anti rabbit igg antibody alkaline phosphatase, by Merck Group (2 mentions) Anti mouse igg whole molecule alkaline phosphatase antibody, by Merck Group (2 mentions) Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (2 mentions) Trans blot turbo transfer system, by Bio-Rad (2 mentions)

Close spelling variants of this product

Monoclonal anti-polyhistidine antibody Anti-polyhistidine antibody Anti-polyHistidine Murine antibody

2 protocols using monoclonal murine anti polyhistidine antibody

1

Immunoblotting of Mycobacterial Proteins

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Proteins were separated on 4–20% gradient SERVA gels and transferred onto a nitrocellulose membrane using a Trans‐Blot® Turbo™ Transfer System (Bio‐Rad). SIGMAFAST™ BCIP®/NBT or SignalFire™ Elite ECL Reagent were used to visualise proteins on C‐DiGit Chemiluminescent Blot Scanner (LI‐COR Biosciences). The following antibodies were used: custom polyclonal antibody raised against Lsr2 in rabbit (Gemini Biosciences); monoclonal murine anti‐polyhistidine antibody (Sigma‐Aldrich); phospho‐threonine antibody (Cell Signaling Technology); monoclonal anti‐MtbGroEL2 (Rv0440), clone IT‐70 (BEIResources); mouse anti‐rabbit IgG antibody:alkaline phosphatase (Sigma‐Aldrich; anti‐mouse IgG (whole molecule:alkaline phosphatase antibody produced in rabbit (Sigma‐Aldrich), and anti‐rabbit IgG, HRP‐linked antibody (Cell Signaling Technology).
Alqaseer K., Turapov O., Barthe P., Jagatia H., De Visch A., Roumestand C., Wegrzyn M., Bartek I.L., Voskuil M.I., O'Hare H.M., Ajuh P., Bottrill A.R., Witney A.A., Cohen‐Gonsaud M., Waddell S.J, & Mukamolova G.V. (2019). Protein kinase B controls Mycobacterium tuberculosis growth via phosphorylation of the transcriptional regulator Lsr2 at threonine 112. Molecular Microbiology, 112(6), 1847-1862.
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2

Western Blot Analysis of Lsr2 Protein

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Proteins were separated on 4–20% gradient SERVA gels and transferred onto a nitrocellulose membrane using a Trans-Blot® Turbo™ Transfer System (Bio-Rad). SIGMAFAST™ BCIP®/NBT or SignalFire™ Elite ECL Reagent were used to visualise proteins on C-DiGit Chemiluminescent Blot Scanner (LI-COR Biosciences). The following antibodies were used: custom polyclonal antibody raised against Lsr2 in rabbit (Gemini Biosciences); monoclonal murine anti-polyhistidine antibody (Sigma-Aldrich); phospho-threonine antibody (Cell Signaling Technology); monoclonal anti-MtbGroEL2 (Rv0440), clone IT-70 (BEIResources); mouse anti-rabbit IgG antibody:alkaline phosphatase (Sigma-Aldrich; anti-mouse IgG (whole molecule:alkaline phosphatase antibody produced in rabbit (Sigma-Aldrich), and anti-rabbit IgG, HRP-linked antibody (Cell Signaling Technology).
Alqaseer K., Turapov O., Barthe P., Jagatia H., De Visch A., Roumestand C., Wegrzyn M., Bartek I.L., Voskuil M.I., O'Hare H.M., Ajuh P., Bottrill A.R., Witney A.A., Cohen‐Gonsaud M., Waddell S.J, & Mukamolova G.V. (2019). Protein kinase B controls Mycobacterium tuberculosis growth via phosphorylation of the transcriptional regulator Lsr2 at threonine 112. Molecular Microbiology, 112(6), 1847-1862.
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