Log In Sign up
The largest database of trusted experimental protocols

Plan apo 100x 1.4na phase contrast oil objective

Manufactured by Nikon
Visit Supplier

The Plan Apo 100x/1.4NA phase contrast oil objective is a high-performance objective lens designed for microscopy applications. It features a numerical aperture of 1.4 and a magnification of 100x, providing excellent optical performance and resolution. The objective is optimized for phase contrast imaging techniques.

Automatically generated - may contain errors

Lab products found in correlation

Metamorph software, by Molecular Devices (3 mentions) Fm4 64, by Thermo Fisher Scientific (2 mentions) Ti2 e microscope, by Nikon (2 mentions) Coolsnap hq2, by Nikon (1 mentions) Tma dph, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Plan Apo 100x oil objective Plan Apo 1.4NA oil Plan Apo 100x/ Plan Apo objective Plan Apo I Plan Apo

3 protocols using plan apo 100x 1.4na phase contrast oil objective

1

Fluorescence Microscopy of Cellular Membranes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fluorescence microscopy was performed on a Nikon Ti2E microscope equipped with Plan Apo 100x/1.4NA phase contrast oil objective and an sCMOS camera. Images were acquired using Nikon Elements software. Cells were immobilized using 2% agarose pads containing growth media. Membranes were stained with FM4–64 (Molecular Probes) at 3 μg/ ml. DNA was stained with DAPI at 2 μg/ml. Images were cropped, linearly adjusted and analyzed using MetaMorph software (Molecular Devices). Final figures were prepared in Adobe Illustrator.
Brandão H.B., Ren Z., Karaboja X., Mirny L.A, & Wang X. (2021). DNA-loop extruding SMC complexes can traverse one another in vivo. Nature structural & molecular biology, 28(8), 642-651.
+ Open protocol
+ Expand
2

Fluorescence Microscopy of Bacterial Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Fluorescence microscopy was performed on a Nikon Ti microscope equipped with Plan Apo 100x/1.4NA phase contrast oil objective and a CoolSnapHQ2 camera. Cells were immobilized using 1.5% agarose pads containing CH medium. Membranes were stained with TMA-DPH (50μM) (Molecular Probes). Exposure times were 400 ms and 800 ms for TMA-DPH and mCherry, respectively. Quantitative image analysis was performed using Oufti [75 (link)]. Meshes were created using the cytoplasmic mCherry images. The length of the long axis of >350 cells was determined and the mean cell length was calculated. Images were cropped and adjusted using MetaMorph software (Molecular Devices). Final figures were prepared in Microsoft PowerPoint.
Brunet Y.R., Wang X, & Rudner D.Z. (2019). SweC and SweD are essential co-factors of the FtsEX-CwlO cell wall hydrolase complex in Bacillus subtilis. PLoS Genetics, 15(8), e1008296.
+ Open protocol
+ Expand
3

Fluorescence Microscopy of Stained Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fluorescence microscopy was performed on a Nikon Ti2E microscope equipped with Plan Apo 100x/1.4NA phase contrast oil objective and an sCMOS camera. Cells were immobilized using 2% agarose pads containing growth media. Membranes were stained with FM4-64 (Molecular Probes) at 3 μg/ ml. DNA was stained with DAPI at 2 μg/ml. Images were cropped and adjusted using MetaMorph software (Molecular Devices). Final figures were prepared in Adobe Illustrator.
Karaboja X., Ren Z., Brandão H.B., Paul P., Rudner D.Z, & Wang X. (2021). XerD unloads bacterial SMC complexes at the replication terminus. Molecular cell, 81(4), 756-766.e8.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!