Log In Sign up
The largest database of trusted experimental protocols

Glass bottomed culture dish

Manufactured by MatTek
Visit Supplier
Sourced in United States

The glass-bottomed culture dish is a laboratory equipment designed for cell culture applications. It provides a transparent glass surface at the bottom of the dish, allowing for direct microscopic observation and imaging of cells grown in the dish.

Automatically generated - may contain errors

Lab products found in correlation

Co2 independent medium, by Thermo Fisher Scientific (2 mentions) 1x81 microscope, by Olympus (1 mentions) Csu x1 spinning disk, by Olympus (1 mentions) Tcs sp5 confocal microscope, by Leica (1 mentions) Co2 independent media, by Thermo Fisher Scientific (1 mentions) Deltavision core microscope system, by Cytiva (1 mentions) Glass bottom culture dish, by MatTek (1 mentions) Ix 70 inverted microscope base, by Olympus (1 mentions) Deltavision microscope system, by Cytiva (1 mentions) Lsm830, by Zeiss (1 mentions) Alexa fluor 647 conjugated goat anti rabbit igg, by Abcam (1 mentions) Alexa fluor 488 conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Lsm image browser software, by Zeiss (1 mentions) Lsm 510 nlo, by Zeiss (1 mentions)

Spelling variants of this product

Glass-bottomed dishes (99 citations) Glass-bottomed culture dishes (33 citations) Glass-bottomed dish (19 citations) 35 mm glass-bottomed culture dish (3 citations)

14 protocols using glass bottomed culture dish

1

Embryo Preparation for Confocal Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Embryos were collected at 25 degrees overnight. They were then dechorionated with 50% bleach and washed with water. Embryos at the right stage were chosen under the fluorescent lamp and mounted depending on the experiment to be performed. For confocal imaging, the embryos were mounted on a glass-bottomed culture dish (Mattek), covered with 1% agarose and immersed in 1% PBS. For stretching experiments, the embryos were mounted on a glass-bottomed culture dish (Mattek) that had been coated with heptane glue, and immersed in 1% PBS. The imaging was carried using a Yokogawa CSU-X1 spinning disk on an inverted Olympus 1X81 microscope, with lens U plan S Apo 60x 1.45 Oil.
Sumi A., Hayes P., D’Angelo A., Colombelli J., Salbreux G., Dierkes K, & Solon J. (2018). Adherens Junction Length during Tissue Contraction Is Controlled by the Mechanosensitive Activity of Actomyosin and Junctional Recycling. Developmental Cell, 47(4), 453-463.e3.
+ Open protocol
+ Expand
2

Cell Death Imaging with HQ5 and Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols
4T1-luc2 cells were cultured in a glass bottomed culture dish (MatTek Corp.) until 80% confluent. Cell death was induced by incubation with GA (3 μM, 1 h). Subsequently, the cells were washed gently with PBS and incubated in the presence of 80 nM HQ5 in the dark for 15 min at RT. AVF and PI were used in accordance with the manufacturer's protocols. Afterwards, samples were imaged using a Leica TCS SP5 confocal microscope (Leica).
Xie B., Stammes M.A., van Driel P.B., Cruz L.J., Knol-Blankevoort V.T., Löwik M.A., Mezzanotte L., Que I., Chan A., van den Wijngaard J.P., Siebes M., Gottschalk S., Razansky D., Ntziachristos V., Keereweer S., Horobin R.W., Hoehn M., Kaijzel E.L., van Beek E.R., Snoeks T.J, & Löwik C.W. (2015). Necrosis avid near infrared fluorescent cyanines for imaging cell death and their use to monitor therapeutic efficacy in mouse tumor models. Oncotarget, 6(36), 39036-39049.
+ Open protocol
+ Expand
3

Live-cell Imaging of HeLa Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa cells were cultured in a glass-bottomed culture dish (MatTek). During imaging, cells were maintained in CO2-independent media (Gibco) containing 10% (vol/vol) FBS and 1% (2 mM) glutamine in a sealed chamber at 37 °C. Images of living cells were taken with a DeltaVision RT system (Applied Precission). Images were prepared for publication using Adobe Photoshop. Measurements and statistical analyses were calculated using ImageJ software (NIH) and GraphPad Prism.
Qin B., Cao D., Wu H., Mo F., Shao H., Chu J., Powell M., Aikhionbare F., Wang D., Fu C., He P., Pan W., Wang W., Liu X, & Yao X. (2016). Phosphorylation of SKAP by GSK3β ensures chromosome segregation by a temporal inhibition of Kif2b activity. Scientific Reports, 6, 38791.
+ Open protocol
+ Expand
4

Measuring NMB Release from Sensory Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols
To detect NMB release from sensory neurons, TG neurons were dissociated and seeded at high density on the 7 mm glass window of a glass-bottomed culture dish (MatTek Corporation). Neurons were cultured at 37 °C for 24 hours and were gently rinsed twice with warm Ca2+- and Mg2+-free HBSS before test. To activate the neurons, 50 μL of CIB containing vehicle or stimulants were gently added onto the neurons and incubated at 37 °C for 5 min before the supernatant was collected. NMB concentration in the supernatant was then measured by ELISA (LifeSpan Biosciences, Cat#:LS-F4262) using the manufacturer’s supplied standards and their recommended protocol.
Li F., Jiang H., Shen X., Yang W., Guo C., Wang Z., Xiao M., Cui L., Luo W., Kim B.S., Chen Z., Huang A.J, & Liu Q. (2021). Sneezing reflex is mediated by a peptidergic pathway from nose to brainstem. Cell, 184(14), 3762-3773.e10.
+ Open protocol
+ Expand
5

Live-Cell Fluorescence Microscopy Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fluorescence microscopy images were obtained by using the microscope system (DeltaVision; Applied Precision) comprising a wide-field inverted epifluorescence microscope (IX71; Olympus), a Plan Apochromat 60×, NA 1.4, oil immersion objective (Olympus). DeltaVision image acquisition software (softWoRx 3.3.0; Applied Precision) equipped with a charge-coupled device camera (CoolSNAP HQ; Photometrics) was used. Live cells were imaged in a glass-bottomed culture dish (MatTek Corporation) coated with soybean lectin and incubated at 27°C for most of the strains or at 36°C for the temperature-sensitive mutants. The latter were cultured in rich YE5S media until mid–log phase at 27°C and subsequently shifted to the restrictive temperature of 36°C before observation. To keep cultures at the proper temperature, a temperature-controlled chamber (Precision Control) was used. The sections of images at each time point were compressed into a 2D projection using the DeltaVision maximum intensity algorithm. Deconvolution was applied before the 2D projection. Images were taken as 10–14 sections along the z axis at 0.3-µm intervals; they were then deconvolved and merged into a single projection. Captured images were processed with Photoshop CS5 (version 12.0; Adobe).
Yukawa M., Ikebe C, & Toda T. (2015). The Msd1–Wdr8–Pkl1 complex anchors microtubule minus ends to fission yeast spindle pole bodies. The Journal of Cell Biology, 209(4), 549-562.
+ Open protocol
+ Expand
6

Microscopic Visualization of Sporulating Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were induced to sporulate by resuspension in SM medium (Sterlini and Mandelstam, 1969 (link)). At various time points, 100 μl of culture was harvested and resuspended in 10 μl SM medium containing 5 μg·mL-1 fluorescent dye FM4–64 to visualize membranes, and placed on a glass- bottomed culture dish (Mattek) 3 μl was spotted on a glass bottom culture dish (Mattek) and covered with a 1% agarose pad made with SM medium. Cells were viewed at room temperature with a DeltaVision Core microscope system (Applied Precision/GE Healthcare). Seven planes were acquired every 200 nm, and the data were deconvolved using SoftWorx software (Eswara et al., 2018 ). Linescan analyses were performed using Fiji.
Delerue T., Anantharaman V., Gilmore M.C., Popham D.L., Cava F., Aravind L, & Ramamurthi K.S. (2022). Bacterial developmental checkpoint that directly monitors cell surface morphogenesis. Developmental cell, 57(3), 344-360.e6.
+ Open protocol
+ Expand
7

Oocyte Activation Protocols for Live Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Oocytes were activated with different, but equivalent preparation methods:

Dissected oocytes were placed in series 95 halocarbon oil (KMZ Chemicals) on 22 × 40 coverslips, aligned parallel to each other to maximize the acquisition area for imaging and left to settle for 10–15 min before addition of AB and imaging (Derrick, York‐Andersen, & Weil, 2016); AB contains 3.3 mM NaH2PO4, 16.6 mM KH2PO4, 10 mM NaCl, 50 mM KCl, 5% polyethylene glycol 8000, 2 mM CaCl2, brought to pH 6.4 with a 1:5 ratio of NaOH:KOH (Mahowald, Goralski, & Caulton, 1983).

Oocytes were dissected in IB (Page & Orr‐Weaver, 1997) in a glass‐bottomed Petri dish and activated by replacing IB with modified RB. IB contains 55 mM NaOAc, 40 mM KOAc, 1.2 mM MgCl2, 1 mM CaCl2, 110 mM sucrose, 100 mM HEPES in ddH2O. IB is adjusted to pH 7.4 with NaOH and filter sterilized. RB contains 55 mM NaOAc, 8 mM KOAc, 20 mM sucrose, 0.5 mM MgCl2, 2 mM CaCl2, 20 mM HEPES in ddH2O. RB was adjusted to pH 6.4 with NaOH and filter sterilized (Hu & Wolfner, 2019);

For the high‐resolution 3D live imaging oocytes were mounted in a glass‐bottomed culture dish (MatTek) in Schneider's insect culture medium (GIBCO‐BRL) with a 1 mm2 coverslip on the oocyte. For activation, Schneider's medium was removed and replaced with AB (Weil et al., 2008).

York‐Andersen A.H., Hu Q., Wood B.W., Wolfner M.F, & Weil T.T. (2019). A calcium‐mediated actin redistribution at egg activation in Drosophila. Molecular Reproduction and Development, 87(2), 293-304.
+ Open protocol
+ Expand
8

Visualizing Microtubule Dynamics in Live Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were cultured in a glass-bottomed culture dish (MatTek, MA) pretreated with collagen. Then, the cells were transfected with mCherry-UtroCH/GFP- tubulin and double blocked by Thymidine as described previously. About 8 hours after release, DME medium was replaced with CO2- independent medium (Invitrogen) containing 10% FBS and 1% glutamine in a sealed chamber at 37°C. FBS was added according to drug property, as Lat B is unstable in medium with FBS. MG132 was added 5 minutes before the addition of drugs when the cells entered metaphase. We acquired each image every 1 minute over a timeframe of 1 hour or 1.5 hours with Delta-Vision Real Time System built on an Olympus IX-70 inverted microscope base. The live-cell images were also processed with Image-J.
Lu H., Zhao Q., Jiang H., Zhu T., Xia P., Seffens W., Aikhionbare F., Wang D., Dou Z, & Yao X. (2014). Characterization of Ring-Like F-Actin Structure as a Mechanical Partner for Spindle Positioning in Mitosis. PLoS ONE, 9(10), e102547.
+ Open protocol
+ Expand
9

Fission Yeast Spot Assays and Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fission yeast strains used in this study are listed in Table S5. Standard fission yeast methodologies were followed (Moreno et al., 1991 (link)). Spot assays were carried out by spotting 5–10 μl of cells at a concentration of 2×106 cells/ml after 10-fold serial dilutions onto rich YE5S plates with or without a drug (10 µg/ml TBZ). C-terminal tagging and gene disruption were performed using PCR-generated fragments as described previously (Bähler et al., 1998 (link); Sato et al., 2005 (link)). The minichromosome loss assay was carried out as described previously (Niwa et al., 1989 (link); Tange et al., 2016 (link)).
Fluorescence microscope images were obtained using the DeltaVision microscope system (Applied Precision, Inc.) with a cooled CCD camera CoolSNAP.HQ (Photometrics). Live cells were imaged in a glass-bottomed culture dish (MatTek Corporation) coated with soybean lectin.
Matsuo Y., Maurer S.P., Yukawa M., Zakian S., Singleton M.R., Surrey T, & Toda T. (2016). An unconventional interaction between Dis1/TOG and Mal3/EB1 in fission yeast promotes the fidelity of chromosome segregation. Journal of Cell Science, 129(24), 4592-4606.
+ Open protocol
+ Expand
10

Live Cell Imaging of Mitosis

Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa cells were grown in glass-bottomed culture dish (MatTek, MA). During imaging, cells were maintained in CO2-independent medium (GIBCO BRL) containing 10% FBS and 1% glutamine in a sealed chamber heated to 37 °C. Images at single focal plane were acquired by a DeltaVision deconvolution microscope (Applied Precision Inc.) with a 60X/NA 1.4 PlanApochromat objective (Olympus). To trace chromosomes or kinetochores in mitosis, frames were collected at 4- or 5-min intervals. For tracking MT tips, frames were acquired with a 3-sec interval.
Shao H., Huang Y., Zhang L., Yuan K., Chu Y., Dou Z., Jin C., Garcia-Barrio M., Liu X, & Yao X. (2015). Spatiotemporal dynamics of Aurora B-PLK1-MCAK signaling axis orchestrates kinetochore bi-orientation and faithful chromosome segregation. Scientific Reports, 5, 12204.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!