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Conical polypropylene centrifuge tubes

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Conical polypropylene centrifuge tubes are laboratory equipment designed for use in centrifugation processes. They are made of polypropylene, a durable and chemically resistant material. These tubes are conical in shape, which optimizes their performance during centrifugation.

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4 protocols using conical polypropylene centrifuge tubes

1

Survival of Bacterial Cells Under HOCl Stress

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NR744, NR745, CH0127 and AG190 cells were grown aerobically at 37°C with shaking in 50 ml of LB medium in 500 ml flasks. When cells reached an A600 of ~0.45, 5 ml samples were transferred to conical polypropylene centrifuge tubes (50 ml; Sarstedt) and HOCl (2 mM) was added. Cells were then incubated at 37°C with shaking (150 rpm) at 90° inclination. Samples were taken at various time points after stress, diluted in PBS buffer, spotted on LB agar and incubated at 37°C for 16 h. Cell survival was determined by counting colony-forming units per ml (c.f.u./ml). The absolute c.f.u at time-point 0 (used as 100%) was ~108 cells/ml in all experiments. For strains CH194, CH196 and CH197, the same protocol was used with chloramphenicol (25 μg/ml) and arabinose (0.2%) added to the cultures.
Gennaris A., Ezraty B., Henry C., Agrebi R., Vergnes A., Oheix E., Bos J., Leverrier P., Espinosa L., Szewczyk J., Vertommen D., Iranzo O., Collet J.F, & Barras F. (2015). Repairing oxidized proteins in the bacterial envelope using respiratory chain electrons. Nature, 528(7582), 409-412.
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2

Survival of Bacterial Cells Under HOCl Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols
NR744, NR745, CH0127 and AG190 cells were grown aerobically at 37°C with shaking in 50 ml of LB medium in 500 ml flasks. When cells reached an A600 of ~0.45, 5 ml samples were transferred to conical polypropylene centrifuge tubes (50 ml; Sarstedt) and HOCl (2 mM) was added. Cells were then incubated at 37°C with shaking (150 rpm) at 90° inclination. Samples were taken at various time points after stress, diluted in PBS buffer, spotted on LB agar and incubated at 37°C for 16 h. Cell survival was determined by counting colony-forming units per ml (c.f.u./ml). The absolute c.f.u at time-point 0 (used as 100%) was ~108 cells/ml in all experiments. For strains CH194, CH196 and CH197, the same protocol was used with chloramphenicol (25 μg/ml) and arabinose (0.2%) added to the cultures.
Gennaris A., Ezraty B., Henry C., Agrebi R., Vergnes A., Oheix E., Bos J., Leverrier P., Espinosa L., Szewczyk J., Vertommen D., Iranzo O., Collet J.F, & Barras F. (2015). Repairing oxidized proteins in the bacterial envelope using respiratory chain electrons. Nature, 528(7582), 409-412.
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3

Evaluating HOCl Sensitivity in E. coli

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Cells (MG1655 and BE107) were grown at 37°C with shaking in 10 ml of LB (in 100 ml flasks). When cells reached an A600 of ~0.8, 5 ml samples were transferred to conical polypropylene centrifuge tubes (50 ml, Sarstedt) and HOCl (2 mM) was added. After 5 min of incubation, samples were taken and diluted in PBS buffer to ~2.103 cells/ml. 100 μl aliquots were then spread on LB agar plates containing SDS (1%). Colonies were counted the next day.
Gennaris A., Ezraty B., Henry C., Agrebi R., Vergnes A., Oheix E., Bos J., Leverrier P., Espinosa L., Szewczyk J., Vertommen D., Iranzo O., Collet J.F, & Barras F. (2015). Repairing oxidized proteins in the bacterial envelope using respiratory chain electrons. Nature, 528(7582), 409-412.
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4

Evaluating HOCl Sensitivity in E. coli

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells (MG1655 and BE107) were grown at 37°C with shaking in 10 ml of LB (in 100 ml flasks). When cells reached an A600 of ~0.8, 5 ml samples were transferred to conical polypropylene centrifuge tubes (50 ml, Sarstedt) and HOCl (2 mM) was added. After 5 min of incubation, samples were taken and diluted in PBS buffer to ~2.103 cells/ml. 100 μl aliquots were then spread on LB agar plates containing SDS (1%). Colonies were counted the next day.
Gennaris A., Ezraty B., Henry C., Agrebi R., Vergnes A., Oheix E., Bos J., Leverrier P., Espinosa L., Szewczyk J., Vertommen D., Iranzo O., Collet J.F, & Barras F. (2015). Repairing oxidized proteins in the bacterial envelope using respiratory chain electrons. Nature, 528(7582), 409-412.
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