There are totally 40 female C57BL/6 mice, aged 8–12 weeks, were used in this study. After arriving, the mice were kept in a 12 h light-dark cycle with food and water ad libitum. A sample size of ten animals per group was calculated as the number required for an alpha of 0.05 and a power of 80%. In addition, the number of animals used here and their suffering were minimized. The laboratory workers were blind to treatment allocation during the experiments and analysis. The use of these animals was approved by the Institute of Animal Care and Use Committee of China Medical University (Permit no. CMUIACUC-2019-106), Taiwan, following the Guide for the use of Laboratory Animals (National Academy Press). Mice were subdivided into four groups: Normal group (Group 1: Normal); Cold stress pain group (Group 2: CSP); 2 Hz Electroacupuncture group (Group 3: 2 Hz EA), and Trpv1 knockout group (Group 4: Trpv1−/−). Trpv1−/− mice were purchased (Jackson Lab, Bar Harbor, ME) and backcrossed with C57BL/6 mice for more than 10 generations.
Trpv1 mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
Trpv1−/− mice are genetically modified mice that have a deletion or inactivation of the Trpv1 gene, which encodes the transient receptor potential vanilloid 1 (TRPV1) ion channel. The TRPV1 channel is a non-selective cation channel that is sensitive to various stimuli, including heat, acid, and certain chemical compounds. These mice are used as a research tool to study the role of the TRPV1 channel in various physiological and pathological processes.
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Lab products found in correlation
Wt c57bl 6 mice, by Jackson ImmunoResearch (1 mentions)
Db db mice, by Jackson ImmunoResearch (1 mentions)
Capsazepine, by Bio-Techne (1 mentions)
Capsaicin, by Bio-Techne (1 mentions)
Wild type c57bl 6 mice, by Jackson ImmunoResearch (1 mentions)
Trpa1 mice, by Jackson ImmunoResearch (1 mentions)
Omp cre mice, by Jackson ImmunoResearch (1 mentions)
Male c57bl 6ncr mice, by Japan SLC (1 mentions)
Wild type c57bl 6, by Jackson ImmunoResearch (1 mentions)
C57bl 6j, by Jackson ImmunoResearch (1 mentions)
C57bl 6j wt mice, by Jackson ImmunoResearch (1 mentions)
C57bl 6j mice, by Charles River Laboratories (1 mentions)
17 protocols using trpv1 mice
1
Investigating TRPV1 in Cold-Induced Pain
2
Alzheimer's Disease and TRPV1 Knockout Mice
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3xTg-AD mice (kindly given by Professor Frank M. Laferla, UC-Irvine, Irvine, CA, USA) were homozygous for APPswe, TauP301L, and PS1M146V. These 3xTg-AD mice were mated with TRPV1−/− mice (stock #003770; provided from Jackson Laboratories, Bar Harbor, ME, USA) and offspring with nine genotypes (3xTg-AD−/−/TRPV1+/+, 3xTg-AD−/−/TRPV1+/−, 3xTg-AD−/−/TRPV1−/−, 3xTg-AD+/−/TRPV1+/+, 3xTg-AD+/−/TRPV1+/−, 3xTg-AD+/−/TRPV1−/−, 3xTg-AD+/+/TRPV1+/+, 3xTg-AD+/+/TRPV1+/−, 3xTg-AD+/+/TRPV1−/−) were generated. Four groups were selected with or without AD pathology and TRPV1 depletion. The selected groups were 3xTg-AD−/−/TRPV1+/+ mice (wild type, WT), 3xTg-AD+/+/TRPV1+/+ mice, 3xTg-AD+/+/TRPV1+/− mice and 3xTg-AD+/+/TRPV1−/− mice. Mice in each group were raised until 12 months. Mice were maintained at 21°C ± 2°C, 12-h light:12-h dark photoperiod with specific pathogen-free conditions in ventilated cages and provided with free choice to consume food and water. All animal testing was performed following protocols approved by the Institutional Animal Care and Use Committee of Seoul National University (SNU-140410-12, SNU-140410-14, SNU-140625-2).
3
Investigating TRPV1 and Obesity in Mice
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All mice (C57BL/6 WT mice, TRPV1-/- mice and db/db mice) were purchased from Jackson Laboratory (Bar Harbor, Maine, USA). TRPV1-/- mice which TRPV1 gene was knocked out in the whole body, not conditioned knockout we used in the experiments. WT and TRPV1-/- male mice were randomized into three groups respectively (n = 15, 5 mice/cage) at 4-6 weeks of age, one group received standard laboratory chow, one group received high-fat diet, one group received high-fat diet plus 0.01% capsaicin. The high-fat diet supplied 49% of the calories as fat and 30% of the calories as carbohydrate. Db/db male mice were randomized into two groups respectively (n = 6, 6 mice/cage) at 4-6 weeks of age, one group received standard laboratory chow, the other group received standard laboratory chow plus 0.01% capsaicin. The standard laboratory chow diet provided 10% of the calories as fat and 68% as carbohydrate. All mice were housed in colony cages with a 12-hour light/12-hour dark cycle with free access to food and water for 5 months, body weight was measured every two weeks. At the end of 5 months, blood glucose was measured. Visceral fat was removed and weighted. Serum lipids were analyzed using routine techniques. All of the experiment procedures were performed in accordance with protocols approved by the Institutional Animal Care and Research Advisory Committee.
4
TRPV1 Knockout Mice for Pain Research
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All experiments were approved by the Committee of Use of Laboratory Animals and Common Facility, Institute of Neuroscience, Chinese Academy of Sciences and carried out according to the guidelines of the International Association for the Study of Pain. All animals were housed under a 12-h light/dark cycle at 22-26°C, with free access to food and water. Sprague–Dawley male rats were bought from Shanghai SLAC Laboratory Animal CO. LTD (SLAC). TRPV1 knockout (TRPV1−/−) mice were bought from the Jackson Laboratory (JAX). The strain name is B6.129X1-TRPV1tm1jul/J and the stock number is 003770. An exon encoding part of the fifth and all of the sixth putative trans-membrane domains together with the pore-loop region was disrupted [20 (link)]. The heterozygotes were bred to obtain TRPV1−/− mice and their wild-type littermates (TRPV1+/+). TRPV1−/− mice were viable and fertile. Capsaicin and capsazepine were purchased from Tocris Bioscience.
5
TRPV1 Knockout Mice in ICS Study
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All mice were treated in accordance with the National Institute of Health Guide for the Care and Use of Laboratory Animals. The study protocol was approved by the Ethics Committee of the China Medical University, Taichung, Taiwan (permit no. CMUIACUC-2021-012). All C57BL/6 female mice weighing approximately 18–23 g and aged 8–12 weeks were used in this study. Totally, 36 female mice were used, including 27 wild-type (WT) mice (BioLASCO Taiwan Co., Ltd.) and 9 Trpv1−/− mice (Jackson Lab, Bar Harbor, ME). A sample size of nine animals per group was calculated as the number required for an alpha of 0.05 and a power of 80%. In addition, the number of animals used here and their suffering were minimized. Mice were placed in Plexiglas cages and housed in a 12 : 12-hour light-dark cycle (8.00 a.m.–8.00 p.m.) room under temperature (25 ± 2°C) and relative humidity (60 ± 5%) controlling. All WT mice were randomly subdivided into 3 groups: control (Con) group, ICS group, and ICS with electroacupuncture (ICS + EA) group. The final group is TRPV1 knockout mice, which were exposed to ICS without treatment (ICS + KO). To conform with the 3Rs Principle of Humane Experimental Technique, we do not set the sham group (ICS + Sham) according to previous articles.
6
Neonatal and Adult Mice Studies
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The studies were performed in neonatal and adult C57BL/6JOlaHsd male mice and in adult (1–3 months) TRPV1−/− mice (Jackson Laboratory) and Wistar male adult (3–4 months) rats. All experimental procedures were performed according to the Spanish Royal Decree 1201/2005 and the European Community Council directive 2010/63/EU. The Ethics Committee from Universidad Miguel Hernández, Alicante, Spain, approved this study.
7
Trpv1 Knockout Mice Experiments
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Experiments were performed using 2–3-month-old male C57BL/6 wild-type mice and Trpv1−/− mice (Jackson Laboratories, Bar Harbor, Main, USA). Mice were housed under a 12 hr/12 hr day/night cycle with free access to food and water. The investigation was approved by the local government authorities and conforms to the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health and the ethics policies of our University and the Land Berlin. The protocol was approved by the Committee on the Ethics of Animal Experiments of the Charité and Land Berlin (Permit Number: 0065/11).
8
Genetic Mouse Models in Pain Research
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Male C57BL/6NCr mice were purchased from Japan SLC, Inc (Shizuoka, Japan). The Trpa1−/− mice (stock number 006401), Trpv1−/− mice (stock number 003770), Trpa1flox mice (stock number 008649), Omp-Cre mice (stock number 006668), RCL-GCamP6f mice (stock number 028865), and RCL-ChR2(H134R)/EYFP mice (stock number 024109) were purchased from The Jackson Laboratory (Bar Harbor, ME, USA). Trpa1-Cre+ mice were generated by inserting improved-Cre54 (link) at the start codon of the endogenous Trpa1 locus using CRISPR/Cas9-mediated genome editing in the ES cells55 (link). RCL-ChR2(H134R)/EYFP mice were crossed with Trpa1-Cre+ mice to visualize Trpa1-Cre+ cells. The ΔD mice were generated by crossing OMACS-Cre and Eno2-STOP-DTA mice, as reported previously6 (link). Advillin-Cre mice were kindly provided by Dr. Wang31 (link). Mice were housed under a standard 12-h light/dark cycle at room temperature of 23 ± 2 °C with humidity of 30–70% and allowed ad libitum access to food and water. Mice were at least 9 weeks old at the start of testing. The experimental protocols were approved by the Animal Research Committee of Kansai Medical University.
9
Investigating TRPV1 in Mouse Model
10
Transient Receptor Potential Channel Study
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