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V plex human cytokine 30 plex kit

Manufactured by Mesoscale
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The V-PLEX Human Cytokine 30-Plex Kit is a multiplex assay that can quantify the levels of 30 different human cytokines simultaneously from a single sample. It is designed to provide a comprehensive analysis of the human immune system response.

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Lab products found in correlation

Meso quickplex sq 120, by Mesoscale (2 mentions) Dulbecco phosphate buffered saline (dpbs), by Merck Group (1 mentions) Triton x 100, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Accuri c6, by BD (1 mentions) Meso scale discovery platform, by Mesoscale (1 mentions) Discovery workbench 4, by Mesoscale (1 mentions) Sector imager 6000, by Mesoscale (1 mentions) Sunrise reader, by Tecan (1 mentions) Quickplex sq 120, by Mesoscale (1 mentions) Edta treated tube, by BD (1 mentions) Meso quickplex sq 120 reader, by Mesoscale (1 mentions) Magnetic bead based multiplex assay, by R&D Systems (1 mentions)

19 protocols using v plex human cytokine 30 plex kit

1

Cytokine Analysis in Plasma

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Peripheral blood plasma collected under standard protocols was stored at −80°C. Concentrations of 30 cytokines/chemokines were analyzed in duplicate using the Meso Scale Discovery platform with the V-PLEX human cytokine 30-plex kit (Meso Scale Discovery, Rockville, MD).
Fisher D.A., Malkova O., Engle E.K., Miner C., Fulbright M.C., Behbehani G.K., Collins T.B., Bandyopadhyay S., Zhou A., Nolan G.P, & Oh S.T. (2016). Mass Cytometry Analysis Reveals Hyperactive NF Kappa B Signaling in Myelofibrosis and Secondary Acute Myeloid Leukemia. Leukemia, 31(9), 1962-1974.
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2

Cytokine Analysis in Plasma

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Peripheral blood plasma collected under standard protocols was stored at −80°C. Concentrations of 30 cytokines/chemokines were analyzed in duplicate using the Meso Scale Discovery platform with the V-PLEX human cytokine 30-plex kit (Meso Scale Discovery, Rockville, MD).
Fisher D.A., Malkova O., Engle E.K., Miner C., Fulbright M.C., Behbehani G.K., Collins T.B., Bandyopadhyay S., Zhou A., Nolan G.P, & Oh S.T. (2016). Mass Cytometry Analysis Reveals Hyperactive NF Kappa B Signaling in Myelofibrosis and Secondary Acute Myeloid Leukemia. Leukemia, 31(9), 1962-1974.
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3

Plasma Cytokine Quantification by Electrochemiluminescence

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Electrochemiluminescence-based immunoassay was used to measure cytokine levels in plasma. Samples and standards were prepared on multispot 96-well plates from the V-PLEX® Human Cytokine 30-Plex Kit (Meso Scale Discovery®, Rockville, MD, USA) per the manufacturer’s instructions. Plates were then analyzed by the Meso Scale Discovery® (MSD) QuickPlex SQ 120 and samples concentrations were calculated using the Discovery Workbench 4.0 using a 4-PL curve fit model. Samples below the lower level of detection, which was calculated by the workbench software as 2.5 standard deviations above the assay background blank, were reported as 0 pg/mL.
Ahmad I.M., Abdalla M.Y., Moore T.A., Bartenhagen L., Case A.J, & Zimmerman M.C. (2019). Healthcare Workers Occupationally Exposed to Ionizing Radiation Exhibit Altered Levels of Inflammatory Cytokines and Redox Parameters. Antioxidants, 8(1), 12.
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4

Cytokine Profiling of LPS-Stimulated Cells

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Samples were treated with 100 ng/ml lipopolysaccharide (LPS) for 24 h and assayed for human chemokine and cytokine biomarkers with V‐PLEX Human Cytokine 30‐Plex Kit (Meso Scale Discovery) following the manufacturer's instructions. The analytes included Eotaxin, Eotaxin‐3, GM‐CSF, IFN‐γ, IL‐1α, IL‐1β, IL‐2, IL‐4, IL‐5, IL‐6, IL‐7, IL‐8, IL‐8 (HA), IL‐10, IL‐12/IL‐23p40, IL‐12p70, IL‐13, IL‐15, IL‐16, IL‐17A, IP‐10, MCP‐1, MCP‐4, MDC, MIP‐1α, MIP‐1β, TARC, TNF‐α, TNF‐β, VEGF‐A. GM‐CSF was excluded from the analyses since it was present in the culture medium.
Chichagova V., Georgiou M., Carter M., Dorgau B., Hilgen G., Collin J., Queen R., Chung G., Ajeian J., Moya‐Molina M., Kustermann S., Pognan F., Hewitt P., Schmitt M., Sernagor E., Armstrong L, & Lako M. (2023). Incorporating microglia‐like cells in human induced pluripotent stem cell‐derived retinal organoids. Journal of Cellular and Molecular Medicine, 27(3), 435-445.
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5

Elution and Cytokine Analysis of NELF

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The Leukosorb strips were eluted after thawing. The full protocol has been described elsewhere (Rebuli et al., 2017 (link)). Briefly, an elution buffer (1% bovine serum albumin [Sigma], 0.05% Triton X‐100 [Sigma], in DPBS [Sigma]) was added to the strip, then centrifuged to remove NELF from strips. NELF was then analyzed using a V‐Plex Human Cytokine 30‐Plex Kit (Meso Scale Diagnostics), according to manufacturer instructions and the minimum manufacturer recommended dilutions.
Rebuli M.E., Stanley Lee A., Nurhussien L., Tahir U.A., Sun W.Y., Kimple A.J., Ebert CS J.r., Almond M., Jaspers I, & Rice M.B. (2023). Nasal biomarkers of immune function differ based on smoking and respiratory disease status. Physiological Reports, 11(3), e15528.
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6

Cytokine Profiling of Activated iMGL

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iMGL culture media was replaced with basal media for 2 hours prior to stimulation with IFNγ (20 ng/ml), IL1β (20 ng/ml), and LPS (100 ng/ml) for 24 hours, after which cells were collected for RNA and conditioned media assessed for cytokine secretion. To simultaneously assess multiple cytokine and chemokine analytes from iMGL conditioned media, conditioned media from each treatment group was processed and analyzed using the V-PLEX human cytokine 30-plex kit (Mesoscale) per the manufacturer’s protocol.
Abud E.M., Ramirez R.N., Martinez E.S., Healy L.M., Nguyen C.H., Newman S.A., Yeromin A.V., Scarfone V.M., Marsh S.E., Fimbres C., Caraway C.A., Fote G.M., Madany A.M., Agrawal A., Kayed R., Gylys K.H., Cahalan M.D., Cummings B.J., Antel J.P., Mortazavi A., Carson M.J., Poon W.W, & Blurton-Jones M. (2017). iPSC-derived human microglia-like cells to study neurological diseases. Neuron, 94(2), 278-293.e9.
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7

Multiplex Profiling of Plasma Cytokines

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Chemokine and cytokine levels in plasma were assessed by Meso Scale Discovery (MSD) using the V-plex human cytokine 30-plex kit (Eotaxin, Eotaxin-3, GM-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-8 (HA), IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IP-10 (CXCL10), MCP-1 (CCL2), MCP-4 (CCL13), MDC (CCL22), MIP-1α (CCL3), MIP-1β (CCL4), TARC (CCL17), TNF-α, TNF-β, VEGF-A), complemented with Human IL-1RA (V-plex), Human IL-18 (U-plex), and Human CXCL9 (R-plex) kits.
Malengier-Devlies B., Filtjens J., Ahmadzadeh K., Boeckx B., Vandenhaute J., De Visscher A., Bernaerts E., Mitera T., Jacobs C., Vanderbeke L., Van Mol P., Van Herck Y., Hermans G., Meersseman P., Wilmer A., Gouwy M., Garg A.D., Humblet-Baron S., De Smet F., Martinod K., Wauters E., Proost P., Wouters C., Leclercq G., Lambrechts D., Wauters J, & Matthys P. (2022). Severe COVID-19 patients display hyper-activated NK cells and NK cell-platelet aggregates. Frontiers in Immunology, 13, 861251.
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8

Cytokine Profiling in Plasma

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Peripheral blood plasma collected under standard protocols was stored at −80°C. Concentrations of 30 cytokines/chemokines were analyzed in duplicate using the Meso Scale Discovery platform with the V-PLEX Human Cytokine 30-Plex Kit (Meso Scale Discovery). Statistical analysis was performed using Prism (GraphPad Software).
He F., Laranjeira A.B., Kong T., Lin S., Ashworth K.J., Liu A., Lasky N.M., Fisher D.A., Cox M.J., Fulbright M.C., Antunes-Heck L., Yu L., Brakhane M., Gao B., Sykes S.M., D’Alessandro A., Di Paola J, & Oh S.T. (2024). Multiomic profiling reveals metabolic alterations mediating aberrant platelet activity and inflammation in myeloproliferative neoplasms. The Journal of Clinical Investigation, 134(3), e172256.
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9

Multiplex Analysis of Plasma Cytokines

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Chemokine and cytokine levels in plasma were assessed according to manufacturer instructions (Meso Scale Discovery) using the V-plex human cytokine 30-plex kit, complemented with Human IL-1RA (V-plex) and human IL-18 (U-plex) kits.
Penttilä P.A., Van Gassen S., Panovska D., Vanderbeke L., Van Herck Y., Quintelier K., Emmaneel A., Filtjens J., Malengier-Devlies B., Ahmadzadeh K., Van Mol P., Borràs D.M., Antoranz A., Bosisio F.M., Wauters E., Martinod K., Matthys P., Saeys Y., Garg A.D., Wauters J, & De Smet F. (2021). High dimensional profiling identifies specific immune types along the recovery trajectories of critically ill COVID19 patients. Cellular and Molecular Life Sciences, 78(8), 3987-4002.
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10

Cytokine Profiling in BAL Samples

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The V-PLEX Human Cytokine 30-Plex Kit (Meso Scale Diagnostics, Rockville, Maryland, USA) was used to assay interleukin (IL)-1α, IL-6, IL-8, tumour necrosis factor (TNF)-α, MCP-1, and macrophage inflammatory protein (MIP)-1β in BAL samples following the manufacturer’s protocol. See Ryu et al., 2020 for detailed methods 22 (link).
Robinson A., Huff R.D., Ryu M.H, & Carlsten C. (2023). Variants in transient receptor potential channels and toll-like receptors modify airway responses to allergen and air pollution: a randomized controlled response human exposure study. Respiratory Research, 24, 218.
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