Sars cov 2 s antibody

Western blot was performed using an anti-SARS-CoV-2 S antibody and an anti-actin antibody, as previously described [36 (link)]. Briefly, after transfection for 36 h, effector cells bearing S protein on their surface were collected. Samples were prepared to run an SDS-PAGE in 10% gels (Bio-Rad, Hercules, CA) and then transfer onto PVDF membranes. Membranes were blocked with 5% BSA in PBST for 2 h, followed by incubation with a SARS-CoV-2 S antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62), or a beta-actin mouse McAb (ProteinTech, Manchester, UK, Cat: 66009-1-Ig), for another 2 h at room temperature. Horseradish peroxidase (HRP)-conjugated polyclonal Goat anti-Rabbit IgG (1:5000) (DAKO, Cat: P0448) and Goat anti-Mouse IgG (1:5000) (Abcam, Cambridge, UK, Cat: ab6789) were used as secondary antibodies. Proteins were visualized using one-step ECL substrates (Meilunbio, Dalian, China).

Western blot was performed using an anti-SARS-CoV-2 S antibody and an anti-actin antibody, as previously described [36 (link)]. Briefly, after transfection for 36 h, effector cells bearing S protein on their surface were collected. Samples were prepared to run an SDS-PAGE in 10% gels (Bio-Rad, Hercules, CA) and then transfer onto PVDF membranes. Membranes were blocked with 5% BSA in PBST for 2 h, followed by incubation with a SARS-CoV-2 S antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62), or a beta-actin mouse McAb (ProteinTech, Manchester, UK, Cat: 66009-1-Ig), for another 2 h at room temperature. Horseradish peroxidase (HRP)-conjugated polyclonal Goat anti-Rabbit IgG (1:5000) (DAKO, Cat: P0448) and Goat anti-Mouse IgG (1:5000) (Abcam, Cambridge, UK, Cat: ab6789) were used as secondary antibodies. Proteins were visualized using one-step ECL substrates (Meilunbio, Dalian, China).