For cell culture, fetal bovine serum (FBS) was purchased from Hyclone (Piscataway, NY, U.S.A.). Horse serum, penicillin, streptomycin, and MTT were purchased from Invitrogen (Carlsbad, CA, U.S.A.). Dulbecco’s modified Eagle’s medium (DMEM), L-glutamine, and LPS were purchased from Sigma-Aldrich Co. (St. Louis, MO, U.S.A.). For the animal study, 6-hydroxydopamine (6-OHDA) was purchased from Sigma-Aldrich Co. and diluted in saline containing 0.02% ascorbic acid. Desipramine (25 mg/kg) and 3,4-dihydroxyphenyl-L-alanine (L-DOPA, 20 mg/kg) were purchased from Sigma-Aldrich Co. and dissolved in saline. d-Amphetamine (d-AMPH) was purchased from USP (Rockville, MD, U.S.A.) and dissolved in saline. Compound 2 was dissolved in saline containing 0.25% ethanol.
3 4 dihydroxyphenyl l alanine l dopa
Manufactured by Merck Group
Sourced in United States
3,4-dihydroxyphenyl-L-alanine (L-DOPA) is a chemical compound that occurs naturally in the body. It is a precursor to the neurotransmitters dopamine, norepinephrine, and epinephrine.
Automatically generated - may contain errors
Lab products found in correlation
6 hydroxydopamine 6 ohda, by Merck Group (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Horse serum, by Thermo Fisher Scientific (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Thermo Fisher Scientific (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Desipramine, by Merck Group (1 mentions)
Sephadex g 100, by GE Healthcare (1 mentions)
Polyethylene glycol peg 6000, by Merck Group (1 mentions)
Lysozyme, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Ovalbumin (ova), by Merck Group (1 mentions)
Acrylamide, by Merck Group (1 mentions)
Horseradish peroxidase, by Merck Group (1 mentions)
Ammonium persulphate, by Merck Group (1 mentions)
α chymotrypsinogen a, by Merck Group (1 mentions)
Ammonium sulphate, by Merck Group (1 mentions)
Sodium alginate, by Merck Group (1 mentions)
2 protocols using 3 4 dihydroxyphenyl l alanine l dopa
1
Cell Culture and Animal Study Reagents
2
Protein Purification and Analysis
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Polyethylene glycol (PEG 6000), ammonium sulphate, sodium chloride, lysozyme, horse radish peroxidase, α-chymotrypsinogen A, acrylamide, ovalbumin, bovine serum albumin, N,N,N1,N1-tetramethylethylenediamine (TEMED), ammonium persulphate, sodium dodecyl sulphate (SDS), Coomassie brilliant blue R-250, sodium alginate, glutaraldehyde and 3,4-dihydroxyphenyl-L-alanine (L-DOPA), were obtained from Sigma Chemical Company, St Louis, USA. Protein markers for SDS-PAGE were obtained from Carl Roth, Karlsruhe, Germany. Sephadex G-100 was obtained from GE Healthcare Bio-sciences (GHB), Uppsala, Sweden. Other reagents used were of analytical grade.
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