Bacteria collected with swabs were suspended in 10 ml of particle-free sterilized water and trapped on an autoclaved polycarbonate membrane filter (pore size 0.2 μm; Advantec, Tokyo, Japan) in a funnel (filtration area 13 mm2; KGS-04, Advantec). Bacterial cells on the filter were stained with filter-sterilized 1× SYBR Green II nucleic acid staining dye (Invitrogen, Carlsbad, CA, USA). We enumerated fluorescently stained bacterial cells under an epifluorescent microscope (E-400; Nikon, Tokyo, Japan) with the Nikon filter sets B2-A (EX450–490, DM505, BA520). One-hundred microscopic fields (1 mm2) per sample were observed.
Autoclaved polycarbonate membrane filter
Manufactured by Advantec
Sourced in Japan
The Autoclaved Polycarbonate Membrane Filter is a laboratory equipment designed for filtration applications. It is made of polycarbonate material and can be autoclaved for sterilization purposes. The filter provides a physical barrier to separate particles or substances from a liquid or gas medium.
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Lab products found in correlation
2 protocols using autoclaved polycarbonate membrane filter
1
Quantifying Bacterial Cells by Epifluorescence
2
Bacterial DNA Extraction from Water
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Bacterial cells present in 50 mL of the PWD water were trapped onto an autoclaved polycarbonate membrane filter (pore size of 0.2 μm; Advantec, Tokyo, Japan). Bacterial DNA was extracted with the method described in Ichijo et al.2 (link). The DNA was finally eluted with 50 µL of TE buffer.
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