Ki 67 protein

The following substances were used: 1,8-cineole, sodium acetate, Alcian Blue, atropine, thiobarbituric acid, trichloroacetic acid, 5,5’-dithiobis (2-nitrobenzoic acid), bethanechol, carbenoxolone, EDTA, glutathione, histamine, N-acetylcysteine, N-ethylmaleimide, nitro_ω-L-arginine methyl ester, pantoprazole, pentagastrin, ranitidine, sodium lauryl sulfate, 1,1,3,3-tetramethoxypropane, hexadecyltrimethylammonium bromide, 3,3´,5,5´-tetramethylbenzidine, dimethylformamide (Sigma-Aldrich, St. Louis, USA), tris (hydroxymethyl) aminomethane, acetic acid, hydrochloric acid, ethanol, n-butanol, magnesium chloride, sodium chloride, potassium chloride, glucose, sodium hydroxide, anhydrous sodium sulfate, polysorbate 80—Tween 80, hydrogen peroxide solution (Vetec, Duque de Caxias, Brazil), ethyl ether, formaldehyde, phenolphthalein (FMaia, Cotia, Brazil), xylazine, ketamine (Vetbrands, Paulinia, Brazil), PCNA antibody [PC10]—mouse monoclonal antibody (Abcan Inc., Cambridge, US), Ki-67 protein (code: sc-23900, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and BrdU protein (code: sc-32323, Santa Cruz Biotechnology, Santa Cruz, CA, USA). For the purposes of the experiment, the 1,8-cineole (CIN) was emulsified in a Tween 80 at 1% before administration to the animals.

All the reagents below were of analytical grade, and the stock solutions and buffers were prepared with Milli-Q water (Millipore, Billerica, MA, USA). Ethanol, DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt), TPTZ (2,4,6-tris (2-pyridyl)-s-triazine), tris(hydroxymethyl)aminomethane, sodium chloride, ascorbic acid, Griess reagent, lansoprazole, ranitidine hydrochloride, 2-thiobarbituric acid, EDTA (ethylene diamine tetraacetic acid), N-acetylcysteine, quercetin, gallic acid, ellagic acid, tannic acid and catechin, IU urease (jack bean urease, type III), Alcian blue, N-ethylmaleimide, N_ω-nitro-L-arginine methyl ester, ranitidine, carbenoxolone were obtained from Sigma-Aldrich (St. Louis, MO, USA). Trolox is obtained from Merck, Düsseldorf, Germany; TNF alpha ELISA kit is from eBioscience, San Diego, CA, USA. Historesin is obtained from Leica Biosystems, Wetzlar, Germany, and acetic acid and glucose were obtained from Vetec, Duque de Caxias, Rio de Janeiro, Brazil. Ethyl ether, formaldehyde, and phenolphthalein are from FMaia, Cotia, São Paulo, Brazil; xylazine and ketamine are obtained from Vetbrands, Paulínia, São Paulo, Brazil. Ki-67 protein (code: sc-23900) and BrdU protein (code: sc-32323) are from Santa Cruz Biotechnology, Santa Cruz, CA, USA.