Sq456 gc ms

The analytical-scale pyrolysis equipment Pyrolab2000 (Pyrol AB, Sweden) was adopted using a platinum foil pulse pyrolyzer and 580°C isothermal pyrolysis temperature under He atmosphere (Ohra-aho et al., 2005 (link); Kuuskeri et al., 2016 (link)). The system was directly connected to a Bruker Scion SQ 456-GC/MS equipped with an Agilent DB-5MS UI (5%-phenyl)-methylpolysiloxane (30 m × 0.250 mm × 0.25 μm film) capillary column. The injector temperature was 250°C, ion source was 250°C with electron ionization of 70 eV, the MS scan range was m/z 40–400, and helium was the carrier gas at the flow rate of 1 ml/min and 1:2 split ratio. Products were identified with comparison to selected reference compounds with their retention times and mass spectra and by referencing the National Institute of Standards and Technology (NIST) spectral library and literature (Kuuskeri et al., 2016 (link)). AMDIS software (version 2.70, NIST, USA) was used for identification and deconvolution of peaks.