Agilent zorbax eclipse plus c18 column

Manufactured by Agilent Technologies

Sourced in United States, United Kingdom

The Agilent Zorbax Eclipse Plus C18 column is a reversed-phase high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of organic compounds. The column features a spherical silica-based stationary phase with a C18 alkyl bonded ligand, providing high-efficiency and reproducible separations.

Automatically generated - may contain errors

Lab products found in correlation

21 protocols using agilent zorbax eclipse plus c18 column

Adsorption Study of Adsorbent Using SEM, TEM, and HPLC

Check if the same lab product or an alternative is used in the 5 most similar protocols

All pH measurements were carried out using an OHAUS ST series pen pH meter. The adsorption studies were carried out using the Branson 5800 Ultrasonic Cleaner (Danbury, CT, USA). A scanning electron microscopy (SEM, TESCAN VEGA 3 XMU, LMH instrument, Tescan Company, Brno, Czech Republic) coupled with energy dispersive X-ray spectroscopy (EDS) was used to study the morphology and elemental composition of the adsorbent at an accelerating voltage of 20 kV. The transmission electron microscopic image was captured using transmission electron microscopy (TEM, JEM-2100, JEOL, Tokyo, Japan).
An Agilent high-performance liquid chromatography (HPLC) 1200 Infinity series, equipped with a photodiode array detector (Agilent Technologies, Waldbronn, Germany), was used for all analyses. The separation was carried out using an Agilent Zorbax Eclipse Plus C18 column (3.5 μm × 150 mm × 4.6 mm) (Agilent, Newport, CA, USA) operated at an oven temperature of 25 °C. The chromatograms were recorded using a 1.00 mL min⁻¹ flow rate and solvent mixture of 55% mobile phase A (water) and 45% mobile phase B (acetonitrile), and adsorption wavelengths of 230, 260, 280 and 288 nm using an isocratic elution system were used.

Mpupa A., Nqombolo A., Mizaikoff B, & Nomngongo P.N. (2021). Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis. Molecules, 27(1), 248.

+ Open protocol

+ Expand

UHPLC-QTOF-MS/MS Characterization of Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

UHPLC-QTOF-MS/MS data were obtained from an Agilent 6540 UHD Accurate-Mass Q-TOF LC-MS system coupled to an Agilent 1290 Infinity UHPLC system (Agilent, Cheshire, UK). Separation was achieved using an Agilent Zorbax Eclipse Plus C18 column (100 mm × 2.1 mm, 1.8 μm) (Agilent, Cheadle, UK). Mobile phases consisted of acetonitrile (containing 1% formic acid) and 1% formic acid in water. The column temperature was set at 40°C and data were acquired for 5.5 min. The flow rate was (0.6 mL/min). The gradient was set at 5–70% acetonitrile over 3.5 min, then increased to 95% acetonitrile in 1 min and held for 0.5 min before returning to 5% acetonitrile in 0.5 - min. QTOF-MS data were acquired in positive ion mode scanning from m/z 100–1000 with and without auto MS/MS fragmentation. Ionization was achieved with an Agilent JetStream electrospray source and infused internal reference masses. The ion source parameters were gas temperature 325°C, drying gas 10 L/min and sheath gas temperature 400°C. Internal reference ions at m/z 121.05087 and m/z 922.00979 were used for calibration purposes. The sample concentration was 10 μg/mL and dissolved in methanol.

Brandt S.D., Kavanagh P.V., Westphal F., Stratford A., Elliott S.P., Dowling G, & Halberstadt A.L. (2020). Analytical profile of N-ethyl-N-cyclopropyl lysergamide (ECPLA), an isomer of lysergic acid 2,4-dimethylazetidide (LSZ). Drug testing and analysis, 12(10), 1514-1521.

+ Open protocol

+ Expand

Comprehensive Analytical Methodology for Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

VCD and cellular viability were determined using an automated cell counting system (Cedex XS, Roche Innovatis, Germany) using trypan blue staining. Extracellular concentrations of d‐glucose and ʟ‐lactate were monitored using an amperometric biosensor system (LaboTRACE, Trace Analytics, Germany). Extracellular antibody concentrations (anti‐IL‐8 IgG1) were determined using an enzyme‐linked immunosorbent assay (ELISA), as described previously [21 (link)]. Extracellular MTA was quantified using the method described in Verhagen et al., 2020a [28 (link)].
Extracellular amino acid concentrations were measured using an Agilent 1200 HPLC system based on a bicratic reversed phase liquid chromatography (RPLC) method (Agilent Zorbax Eclipse Plus C18 column 250 × 4.6 mm, 5 μm equipped with an Agilent Zorbax Eclipse Plus C18 guard column 12.5 × 4.6 mm, 5 μm) with automated pre‐column derivatization and fluorometric detection [36 ]. We performed absolute quantification using a standard‐based external calibration and adapted sample dilutions (1 to 8) with γ‐aminobutyric acid (GABA) as an internal standard.

Wijaya A.W., Verhagen N., Teleki A, & Takors R. (2021). Compartment‐specific 13C metabolic flux analysis reveals boosted NADPH availability coinciding with increased cell‐specific productivity for IgG1 producing CHO cells after MTA treatment. Engineering in Life Sciences, 21(12), 832-847.

+ Open protocol

+ Expand

UHPLC-MS/MS Oxylipin Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ten µL of the reconstituted free oxylipin extract was analyzed by UHPLC-MS/MS using an Agilent 1290 Infinity UHPLC system coupled to an Agilent 6460 Triple Quadrupole mass-spectrometer (Agilent Technologies, Santa Clara, CA, USA), equipped with an Agilent ZORBAX Eclipse Plus C18 column (2.1 × 150 mm, 1.8 μm particle size; Agilent Technologies, Santa Clara, CA, USA; Cat #959759-902). The specifics of the UHPLC-MS/MS analyses were described previously [65 (link)]. Optimization parameters and parent and product ion monitoring pairs are described in Table S2. We have provided the raw mass spectra for the standard, blank, and a representative sample for 15-HETE in Figure S1.

Norman J.E., Nuthikattu S., Milenkovic D., Rutledge J.C, & Villablanca A.C. (2023). Sex-Specific Response of the Brain Free Oxylipin Profile to Soluble Epoxide Hydrolase Inhibition. Nutrients, 15(5), 1214.

+ Open protocol

+ Expand

Rare Ginsenoside Profiling in Wild Ginseng

Check if the same lab product or an alternative is used in the 5 most similar protocols

An Agilent HPLC 1260 DAD system (Agilent Technologies, Santa Clara, CA, USA) and Agilent Zorbax Eclipse plus C18 column (4.6 × 100 mm, 3.5 μm, Agilent Technologies) were used for the analysis of rare ginsenosides in wild ginseng CMCs. The detection wavelength was 203 nm, the temperature of the column was 30°C, and the mobile phase was 0.05% trifluoroacetic acid in water and 0.05% trifluoroacetic acid in acetonitrile with a flow rate of 1 mL/min. Standards used for the analysis were ginsenoside 20(S)-Rg3, ginsenoside 20(S)-Rh2, and a mixture of ginsenoside Rk1 and Rg5. Solvents used for the analysis were acetonitrile, methanol, and trifluoroacetic acid. Each standard was weighed and the concentration was adjusted to 0.5 mg/mL using methanol. Freeze-dried wild ginseng CMCs were ground to a fine powder, and 80% of methanol solution was added to 0.5 g of ground wild ginseng CMCs to a volume of 10 mL. This solution was extracted for 2 h under ultrasonic waves, centrifuged, and filtered through a 0.2-μm syringe filter (Toyo Roshi Kaisha, Ltd., Tokyo, Japan) for HPLC analysis.

Kim S.J., Choi H.S., Cho H.I., Jin Y.W., Lee E.K., Ahn J.Y, & Lee S.M. (2015). Protective effect of wild ginseng cambial meristematic cells on d-galactosamine-induced hepatotoxicity in rats. Journal of Ginseng Research, 39(4), 376-383.

+ Open protocol

+ Expand

Quantification of Dictamni Cortex Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

The content of obacunone, fraxinellone, and Dictamnine in the four Dictamni Cortex crude drug samples were quantified by LC/MS. Briefly, the crude drug samples dissolved in methanol (1 µL) was injected into an Agilent ZORBAX Eclipse Plus C18 column (1.8 µm, 3.0 × 100 mm) (Agilent Technologies, Santa Clara, CA, USA), equipped with Agilent ZORBAX Eclipse Plus C18 (1.8 µm, 3.0 x 10 mm) guard column (Agilent Technologies). Gradient elution with solvent system 0.1% formic acid in water and 0.1% formic acid in methanol was used. The mixture of obacunone (Chengdu Must Bio-Technology Co., Ltd., Chengdu, China) (retention time: 11.4 min) and fraxinellone (Chengdu Must Bio-Technology Co., Ltd.) (retention time: 11.9 min) was prepared as the standard and analyzed. Dictamnine (Chengdu Must Bio-Technology Co., Ltd.) (retention time: 5.7 min) was also used as a standard and analyzed. Chemical markers were identified based on the retention time and the UV absorbance that resemble the standards.

Tsang M.S., Shaw P.C., Chu I.M., Cheng L., Wong E.C., Lau D.T., Lam C.W, & Wong C.K. (2019). High-Throughput Immunological Analysis of Dictamni Cortex: Implication in the Quality Control of Herbal Medicine. Molecules, 24(16), 2880.

+ Open protocol

+ Expand

Quantification of Cellular Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

Substrates and products were quantified by harvesting 1 mL of cell suspension via centrifugation (12,100×g, 5 min, RT) at given time points. The supernatants were used for further analysis. The glucose concentration was determined with a test kit from Roche (Roche Diagnostics, Mannheim, Germany). Quantification of amino acids was performed with an Agilent 1200 series apparatus (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent Zorbax Eclipse Plus C₁₈ column (250 × 4.6 mm, 5 µm) protected by an Agilent Zorbax Eclipse Plus C₁₈ guard column (12.5 × 4.6 mm, 5 µm). Automatic precolumn derivatization with ortho-phthaldialdehyde was followed by fluorometric detection (excitation at 230 nm and emission at 450 nm). The elution buffer consisted of a polar phase (10 mM Na₂HPO₄, 10 mM Na₂B₄O₇, 0.5 mM NaN₃, pH 8.2) and a nonpolar phase (45% [v v⁻¹] methanol, 45% [v v⁻¹] acetonitrile). Protocol details were described earlier [16 (link)]. Analytes were quantified using 200 µM l-ornithine as the internal standard to correct variabilities in analytes and a seven-point calibration curve for each component as an external reference standard.

Schwentner A., Feith A., Münch E., Stiefelmaier J., Lauer I., Favilli L., Massner C., Öhrlein J., Grund B., Hüser A., Takors R, & Blombach B. (2019). Modular systems metabolic engineering enables balancing of relevant pathways for l-histidine production with Corynebacterium glutamicum. Biotechnology for Biofuels, 12, 65.

+ Open protocol

+ Expand

Broccoli Seedling Flavonoid Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples of broccoli seedlings were ground and suspended in methanol containing 1% HCl. After centrifugation for 30 min at 11,000× g and 4 °C, 100 μL of the supernatant was used for determination. Flavonoids including gallic acid, protocatechuic acid, protocatechualdehyde, chlorogenic acid, caffeic acid, homoorientin, neohesperidin, hyperoside, quercetin, bergapten, kaempferol, umbelliferone, nobiletin, genistin, psoralen, asiatic acid, rhein, galangin, emodin, and chrysophanol were then detected using LC-MS/MS (API 4000) in accordance with Davuluri et al. [31 (link)]. Metabolites in the extract solution were separated using an Agilent ZORBAX Eclipse Plus C18 column (150 mm × 2.1 mm, 3.5 μm, Agilent Technologies). Solvent A consisted of water with 0.3% (v/v) formic acid, while solvent B consisted of acetonitrile with 0.3% (v/v) formic acid. The LC gradient program was set as follows: 0 min, 20% of solvent B; 1 min, 90% of solvent B; 5 min, 90% of solvent B; 5.1 min, 20% of solvent B; and 10 min, 20% of solvent B; flow rate was 0.3 mL min⁻¹. Ion modes and m/z values of the precursor and product ions for each metabolite are listed in Table S1.

Li R., Zhou Z., Zhao X, & Li J. (2024). Application of Tryptophan and Methionine in Broccoli Seedlings Enhances Formation of Anticancer Compounds Sulforaphane and Indole-3-Carbinol and Promotes Growth. Foods, 13(5), 696.

+ Open protocol

+ Expand

HPLC Analysis of DHEA Concentration

Check if the same lab product or an alternative is used in the 5 most similar protocols

The accurate concentration of DHEA was analyzed on an Agilent 1260 series HPLC (Agilent Technologies Co., Ltd., Santa Clara, CA, USA) equipped with a quaternary pump (G1311C), a diode-array detector (G1315D) and a 4.6 × 150 mm, 5 µm Agilent ZORBAX Eclipse Plus C18 column. The injected volume was set to 50 µL, while the UV-vis wavelength was set to 210 nm. The column temperature was 35 °C. An isometric elution method using methanol and deionized water at the ratio of 75:25 (v/v) as the mobile phase was performed with the flow rate at 1 mL/min. The retention time point for DHEA was 4 min.

Jiang Y., Cheng Y., Xia M., Zhang B., Ding Q., Lu L., Wang J.R, & Mei X. (2022). Dehydroepiandrosterone Cocrystals with Improved Solubility and Bioavailability. Pharmaceutics, 14(11), 2478.

+ Open protocol

+ Expand

UPLC Analysis of Compound Separation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The UPLC analyses were performed on a Waters ACQUITY I-Class UPLC system
(Waters, Milford, USA) consisting of a binary pump, a column manager, an
autosampler, a degasser, and a diode-array detector (DAD). An Agilent Zorbax
Eclipse Plus C18 Column (2.1 × 50 mm, 1.8 μm) (Agilent
Technology, Santa Clara, CA) maintained at 35 °C was used with a mobile
phase consisting of a linear gradient of A (0.02 M acetic ammonium in water) and
B (acetonitrile) under the following conditions: 0 → 1→ 3.5
→ 7 → 7.5 → 8.5 → 9.5 → 10 min,
5% → 10% → 15% → 20%
→ 25% → 70% → 85% →
100%B. The flow rate was 0.3 mL/min and the DAD was operated in the
range of 200–400 nm but 282 nm was used (Chen et al., 2007 (link); Lin et al., 2013 (link)) for displaying chromatograms.

Lin Z., Yang R., Guan Z., Chen A, & Li W. (2014). UPLC Separation and QTof–MS Identification of Major Alkaloids in Plumula Nelumbinis. Phytochemical analysis : PCA, 25(6), 485-494.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!