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Lsm 780 confocal microscope

Manufactured by Nikon
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Sourced in Germany

The Nikon LSM 780 is a high-performance confocal microscope designed for advanced imaging applications. It features a laser scanning system that enables detailed observation and analysis of biological samples at the cellular and subcellular levels. The LSM 780 provides high-resolution, sensitive, and flexible imaging capabilities, making it a versatile tool for researchers in various fields of study.

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Lab products found in correlation

Gaasp detector, by Nikon (1 mentions) Stereo discovery v12 microscope, by Zeiss (1 mentions) Tcs sp5 confocal microscope, by Zeiss (1 mentions) A1 rs inverted laser scanning microscope, by Nikon (1 mentions) Eclipse 80i microscope, by Nikon (1 mentions) Prolong gold antifade, by Thermo Fisher Scientific (1 mentions) A1r confocal microscope, by Nikon (1 mentions) Anti mcherry, by Thermo Fisher Scientific (1 mentions) Anti relmα 500 p214, by Thermo Fisher Scientific (1 mentions) Anti rat igg alexa fluor 568, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Cm3050s cryostat, by Leica (1 mentions) Optiphot fluorescent microscope, by Nikon (1 mentions)

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LSM 780 (7 citations) Confocal microscope (523 citations)

5 protocols using lsm 780 confocal microscope

1

Confocal Microscopy of HEK 293 Cells

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The fixed and immunolabeled untransfected or transfected HEK 293 cells were characterized with an LSM 780 confocal microscope equipped with a Nikon GaAsP detector and an Argon laser (with fluorophore excitation wavelengths of 405, 458, and 514 nm). The resulting confocal and fluorescence microscopy images were captured with ZEN software and analyzed with ImageJ (v 2.0.0-rc-69/1.52i).
Chatterjee A., Cerna Sanchez J.A., Yamauchi T., Taupin V., Couvrette J, & Gorodetsky A.A. (2020). Cephalopod-inspired optical engineering of human cells. Nature Communications, 11, 2708.
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2

Cardiac Development and Morphogenesis

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The tissues for RNA in-situ hybridization were prepared as described above except adding Diethylpyrocarbonate (DEPC) was added to avoid RNAse contamination. RNA in-situ hybridization was performed by the RNA In Situ Hybridization Core at Baylor College of Medicine. The Twist1 (Ma et al., 2005 (link)) and Snai2 (Jiang et al., 1998 (link)) probes were previously described.
Gross heart images were captured by Zeiss SteREO Discovery.V12 microscope. Histology and RNA in situ images were captured by Nikon Eclipse 80i microscope. Immunofluorescence images were captured on a Leica TCS SP5 confocal microscope, a Zeiss LSM 780 confocal microscope or Nikon A1-Rs inverted laser scanning microscope.
Xiao Y., Hill M.C., Zhang M., Martin T.J., Morikawa Y., Wang S., Moise A.R., Wythe J.D, & Martin J.F. (2018). Hippo signaling plays an essential role in cell state transitions during cardiac fibroblast development. Developmental cell, 45(2), 153-169.e6.
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3

Immunohistochemistry Staining Protocol

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Immunohistochemistry was conducted as follows: slides were washed 3 times for 5 minutes each in KPBS, antibodies were diluted in KPBS supplemented with 2% donkey serum and 0.4% Triton-X100 and applied overnight at 4 degrees Celsius. After 3 more washes in KPBS, slides were incubated with secondary antibodies (obtained from Jackson ImmunoResearch (Westgrove, PA) and used at 1:600 final dilution), also in donkey block, now for 45 minutes at room temperature. Three more washes completed the procedure. Where applicable, nuclei were counterstained by Dapi at 1 μg/ml final concentration and slides were embedded in Prolong Gold Antifade (Thermo Fisher Scientific, Waltham, MA) and imaged on either a Zeiss LSM780 confocal microscope or a Nikon A1R+ confocal microscope. The antiserum for Ero1lb (Zito et al., 2010 (link)) was generously provided by Dr. David Ron, the antiserum for G6pc2 (Hutton and Eisenbarth, 2003 (link)) was originally generated by the late Dr. John Hutton and generously provided by Drs. Jay Walters and Howard Davidson.
van der Meulen T., Mawla A., DiGruccio M.R., Adams M.W., Nies V., Dólleman S., Liu S., Ackermann A.M., Cáceres E., Hunter A.E., Kaestner K.H., Donaldson C.J, & Huising M.O. (2017). Virgin Beta Cells Persist throughout Life at a Neogenic Niche within Pancreatic Islets. Cell metabolism, 25(4), 911-926.e6.
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4

Cryoprotection and Immunofluorescence Imaging

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Lung and FALC were prepared by fixation in 4% paraformaldehyde at 4 °C for 1 h, incubating in 20% sucrose overnight for cryoprotection, and embedding in 15% sucrose/7.5% gelatin diluted in PBS. Overall, 12 μm frozen sections were cut using a Leica CM 3050 S cryostat. Sections were pre-blocked/permeabilized in PBS containing 3% BSA and 0.05% Triton X-100 and stained with primary antibodies anti-mCherry (M11217, Invitrogen), anti-Relmα (500-P214, PeproTech), or anti-actin α-smooth muscle FITC conjugated (1A4, Sigma), followed by secondary antibodies anti-rat IgG Alexa Fluor 568 (A11077, Life Technologies) and anti-rabbit IgG Alexa Fluor 488 (A21206, Life Technologies) for 1 h at the room temperature, or directly conjugated CD3 (clone 145-2C11), B220 (clone RA3-6B2), and CD169 (clone 3D6.112). Image acquisition was performed using either a Zeiss LSM 780 confocal microscope and ZEN 2010 acquisition software or a Nikon HCA fluorescence microscope and NIS-Elements 4.30.01 acquisition software for live cell imaging. Adobe Photoshop 11.0.2 was used to adjust brightness, contrast and colour balance (changes were applied to all images in equal measure).
Panova V., Gogoi M., Rodriguez-Rodriguez N., Sivasubramaniam M., Jolin H.E., Heycock M.W., Walker J.A., Rana B.M., Drynan L.F., Hodskinson M., Pannell R., King G., Wing M., Easton A.J., Oedekoven C.A., Kent D.G., Fallon P.G., Barlow J.L, & McKenzie A.N. (2020). Group-2 innate lymphoid cell-dependent regulation of tissue neutrophil migration by alternatively activated macrophage-secreted Ear11. Mucosal Immunology, 14(1), 26-37.
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5

Immunohistochemical Analysis of Rev-erbα, Neuronal, and Glial Markers

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Brain samples were fixed in 4% paraformaldehyde, and then transferred to a grade series of sucrose solution (10, 20, and 30%). Coronal brain sections (a thickness of 20 μm) were blocked with 10% horse serum and 0.5% Triton X-100 in PBS, and then incubated with antibodies to Rev-erbα, NeuN, GFAP, or Iab1 (Supplementary Table 5). After washing with PBS, sections were incubated with secondary antibodies and DAPI. Sections were washed, mounted, and imaged using Zeiss LSM780 confocal microscope (Jena, Germany; for Fig. 1c–e) or Nikon Optiphot fluorescent microscope (Tokyo, Japan; for Figs. 2f and 3f). ImageJ software was used to count Rev-erbα-, NeuN-, GFAP-, and Iab1-positive cells. At least three regions from each section and three sections were imaged for each animal, and three animals were used for each group.
Zhang T., Yu F., Xu H., Chen M., Chen X., Guo L., Zhou C., Xu Y., Wang F., Yu J, & Wu B. (2021). Dysregulation of REV-ERBα impairs GABAergic function and promotes epileptic seizures in preclinical models. Nature Communications, 12, 1216.
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