Cgp54626

Manufactured by Bio-Techne

Sourced in United Kingdom

CGP54626 is a laboratory equipment product offered by Bio-Techne. It is designed for use in scientific research and analysis. The core function of this product is to provide a specific tool or instrument for conducting experiments and investigations in a laboratory setting. No further details are available without the risk of introducing bias or interpretation.

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Lab products found in correlation

20 protocols using cgp54626

Neurotransmitter Receptor Modulation Protocol

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NBQX, APV, WAY 100635, SR 95531, and CGP54626 were from Tocris Biosciences. Kynurenic acid and MDL 100907 were from Sigma-Aldrich.

Sengupta A., Bocchio M., Bannerman D.M., Sharp T, & Capogna M. (2017). Control of Amygdala Circuits by 5-HT Neurons via 5-HT and Glutamate Cotransmission. The Journal of Neuroscience, 37(7), 1785-1796.

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GABA and Copper(II) Receptor Assay

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GABA (γ-aminobutyric acid) and dichloro(1,10-phenanthroline)copper(II) were purchased from Sigma-Aldrich (St. Louis, MO, USA). CGP54626 was from Tocris Bioscience (Ellisville, MO, USA). Lipofectamine 2000 and Fluo4-AM were obtained from Life Technologies (Carlsbad, CA, USA). SNAP-Surface^® Alexa Fluor^® 647 was from New England Biolabs, whereas HaloTag^® Alexa Fluor^®660 was from Promega (Beijing) Biotech Co., Ltd.

Xue L., Sun Q., Zhao H., Rovira X., Gai S., He Q., Pin J.P., Liu J, & Rondard P. (2019). Rearrangement of the transmembrane domain interfaces associated with the activation of a GPCR hetero-oligomer. Nature Communications, 10, 2765.

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Colon Motor Activity Analysis in Mice

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Adult male NL3^R451C and WT mice (25–35 g; 8–12 weeks of age) were euthanized by cervical dislocation. The entire colon (~5 cm in length) was dissected, flushed, and cannulated in an organ bath superfused with physiological saline (at 37°C, 95%O₂/5% CO₂, flow: 5 mL min⁻¹). After a 30 min equilibration, video recordings were acquired using a Logitech Quickcam pro 9000 camera (30 fps, 640 × 840 resolution) in order to measure contractile motor activity for a duration of 3 hr. Motor activity was recorded at baseline (1 hr), with bath‐applied antagonist (1 hr), and during washout (1 hr). Pseudocolored spatiotemporal maps of motor patterns were created and motor pattern frequencies were quantified using in‐house software [Neal et al., 2009; Swaminathan et al., 2016] (Fig. S1). Bicuculline (10 μM, Tocris), gabazine (10 μM, Sigma), and CGP 54626 (100 nM, Tocris) were diluted in saline.

Hosie S., Ellis M., Swaminathan M., Ramalhosa F., Seger G.O., Balasuriya G.K., Gillberg C., Råstam M., Churilov L., McKeown S.J., Yalcinkaya N., Urvil P., Savidge T., Bell C.A., Bodin O., Wood J., Franks A.E., Bornstein J.C, & Hill‐Yardin E.L. (2019). Gastrointestinal dysfunction in patients and mice expressing the autism‐associated R451C mutation in neuroligin‐3. Autism Research, 12(7), 1043-1056.

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Fluorescent Staining Compound Protocol

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Sucrose and sulforhodamine B were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Brilliant Blue FCF was purchased from Wako Pure Chemical Industries, Ltd (Osaka, Japan). Agarose was purchased from Invitrogen (Cat no. 75510-019, Carlsbad, CA, USA). CGP54626 was purchased from Tocris (Bristol, UK).

Jeong Y.T., Oh S.M., Shim J., Seo J.T., Kwon J.Y, & Moon S.J. (2016). Mechanosensory neurons control sweet sensing in Drosophila. Nature Communications, 7, 12872.

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Pharmacological Agents for Behavioral Research

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Baclofen was purchased from Sigma Millipore (Burlington, MA), CGP54626 and clozapine-N-oxide (CNO) were purchased from Tocris Bioscience (Bristol, UK), and cocaine hydrochloride was obtained through Boynton Health Pharmacy at the University of Minnesota (Minneapolis, MN).

Rose T.R., Marron Fernandez de Velasco E., Mitten E.H, & Wickman K. (2022). GIRK channel activity in prelimbic pyramidal neurons regulates the extinction of cocaine conditioned place preference in male mice. Addiction Biology, 28(1), e13256.

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Electrophysiological Investigation of Ion Channels

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Tertiapin-Q (STT-170, Alomone Labs), CGP54626 (1088, Tocris), baclofen (0417, Tocris), linopirdine (1999, Tocris), XE991 (2000, Tocris), PK-THPP (5338, Tocris), spadin (5594, Tocris), and tolbutamide (T0891, dissolution with alcohol, Sigma) were used in whole-cell patch clamp mode. Kynurenic acid (K3375, Sigma), picrotoxin (1128, Tocris), and tetrodotoxin (T-550, Alomone Labs) were used to block synaptic currents. All solutions used in this study were made according to manufacturer’s specifications, and stock solutions of all drugs were dissolved in autoclaved de-ionized water unless specifically stated.

Oh Y., Yoo E.S., Ju S.H., Kim E., Lee S., Kim S., Wickman K, & Sohn J.W. (2023). GIRK2 potassium channels expressed by the AgRP neurons decrease adiposity and body weight in mice. PLOS Biology, 21(8), e3002252.

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Pharmacological Agents for Neuroscience Research

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Baclofen was purchased from Sigma Millipore (Burlington, MA), CGP54626 and clozapine‐N‐oxide (CNO) were purchased from Tocris Bioscience (Bristol, UK), and cocaine hydrochloride was obtained through Boynton Health Pharmacy at the University of Minnesota (Minneapolis, MN).

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Optogenetic Manipulation of Striatal iMSN

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We used optical stimulation to activate iMSNs selectively. Channelrhodopsin-2 (ChR2) and its reporter gene enhanced yellow fluorescent protein (EYFP) were inserted in a double-floxed inverted open reading frame viral vector and stereotaxically injected into the striatum of 1 month-old WT and R6/2 mice crossed with A2A-Cre mice (1.0 mm anterior and 2.0 mm lateral to Bregma, at a depth of 3.1 mm from the dura). After Cre recombination, ChR2-EYFP was selectively expressed in A2A-Cre neurons. To examine evoked GABA responses in GPe neurons the following protocol was used: a slow ramp voltage command (3 s duration) from a holding voltage of −70 mV to +10 mV was first applied. Then the cell was held at the new voltage for 2 s, after which the optical stimulation (470 nm, 1 mW, 0.5 ms) was delivered. After 2 additional s at +10 mV the membrane potential was brought back to the original holding potential. This protocol was repeated 3 times (interval between sweeps was 30 s) and the responses to light stimulation were averaged. SNAP5114 (GAT-3 antagonist, 20 μM), NO711 (GAT-1 antagonist, 10μM), or CGP 54626 (GABA_B receptor antagonist, 1μM) (all from Tocris, Minneapolis, MN) were bath applied (10-20 min) to examine alterations in decay time (90%-10%) of the evoked GABA response measured with Clampfit v10.7 (Molecular Devices, Sunnyvale, CA; RRID:SCR_011323).

Barry J., Sarafian T.A., Watson J.B., Cepeda C, & Levine M.S. (2020). Mechanisms Underlying the Enhancement of GABA Responses in the External Globus Pallidus of R6/2 Huntington’s Disease Model Mice. Journal of neuroscience research, 98(11), 2349-2356.

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Thalamic Network Activity Modulation by AMPK Agonists

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Network activity was recorded in 400 µm thalamic slices within an interface chamber supplied with warm, oxygenated artificial CSF (31–33°C, 95% O₂/5% CO₂). Electrical stimuli were delivered to reticular thalamus with bipolar tungsten electrodes (FHC), while single tungsten electrodes placed in ventrobasal nucleus recorded extracellular activity. Filtered activity (0.1–3 kHz) was digitized and recorded with a Digidata 1440A and pClamp software. AMPK agonists and CGP-54626 (Tocris, Cat. no. 1088) were dissolved in the artificial CSF perfusate. Activity was quantified with custom MATLAB scripts.^{36 (link)} Voltage amplitude thresholds were applied to detect action potentials and were set at 3–4× the root mean square of the trace before stimulation. Oscillation duration was defined as the period between the stimulus onset and the last activity burst to occur within the specified inter-burst interval of 1 s.

Salvati K.A., Ritger M.L., Davoudian P.A., O’Dell F., Wyskiel D.R., Souza G.M., Lu A.C., Perez-Reyes E., Drake J.C., Yan Z, & Beenhakker M.P. (2022). AMPK-mediated potentiation of GABAergic signalling drives hypoglycaemia-provoked spike-wave seizures. Brain, 145(7), 2332-2346.

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Preparation of Common Pharmacological Agents

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Tubocurarine chloride (Tocris) was dissolved in water to make a 25 mM stock
which was stored at 4°C. Imidacloprid (Sigma-Aldrich, item # 37894) was
dissolved in DMSO to make a 1 mM stock which was at room temperature. Methyllycaconitine
citrate salt hydrate (Sigma) was dissolved in water to make a 2mM stock and kept at
4°C. Alpha-bungarotoxin (Tocris) was dissolved in water to make a 5mM stock and
kept at 4°C. Picrotoxin (Tocris) was dissolved in aqueous 100 mM NaCl to make a 5
mM stock solution which was stored in the dark at room temperature. CGP54626 (Tocris) was
dissolved in DMSO to make a 50 mM stock solution which was stored at
−20°C.

Nagel K.I., Hong E.J, & Wilson R.I. (2014). Synaptic and circuit mechanisms promoting broadband transmission of olfactory stimulus dynamics. Nature neuroscience, 18(1), 56-65.

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