Goatanti mouse igg h l antibody

Manufactured by Abcam

Sourced in China

Goat Anti-Mouse IgG H&L antibody is a secondary antibody used in various immunoassays and molecular biology techniques. It is designed to recognize and bind to the heavy and light chains of mouse immunoglobulin G (IgG) antibodies.

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Lab products found in correlation

Ab150113, by Abcam (1 mentions) Brdu solution, by Beyotime (1 mentions) Ab8152, by Abcam (1 mentions) Macsquant analyzer, by Miltenyi Biotec (1 mentions) Ez lysis buffer, by Merck Group (1 mentions) Mouse anti neun antibody, by Merck Group (1 mentions) Dounce homogenizer, by Merck Group (1 mentions)

3 protocols using goatanti mouse igg h l antibody

BrdU Incorporation Assay for Proliferation

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U87/TMZ and U251/TMZ cells were transferred to 24-
well plates. After the cells were cultured in serum-free
medium for 24 hours, the cells were incubated with 10
μmol/L of BrdU solution (Beyotime, Shanghai, China) in
a complete medium for 4 hours at 37°C. After the medium
was removed, the cells were rinsed 3 times with phosphate
buffer saline (PBS), fixed with 70% ethanol for 10 minutes
at 4°C, and then washed 3 times with PBS. Then, 2 mol/L
of HCl was added to denature the cellular DNA at 37°C
for 60 minutes before the cells were washed 3 times with
PBS. After that, the cells were blocked with 3% bovine
serum albumin (KPL, Gaithersburg, MD, USA) at room
temperature for 1 hour, followed by the rinse with PBS 3
times, 5 minutes for each time. Subsequently, the cells were
incubated overnight at 4°C with anti-BrdU monoclonal
antibody (ab8152, 1:300, Abcam, Shanghai, China) and
then with goat anti-mouse fluorescent secondary Goat
Anti-Mouse IgG H&L antibody (ab150113, 1:1000,
Abcam, Shanghai, China) at room temperature for 2 hours.
After counterstaining the cell nucleus with DAPI, the
cells were observed under a fluorescence microscope. In
10 randomly selected non-overlapping fields, the number
of BrdU-positive cells was counted, and the average was
calculated.

Houjun Z, & Peng B. (2023). Down-Regulation of CEND1 Expression Contributes to The Progression and Temozolomide Resistance of Glioma. Cell Journal (Yakhteh), 25(4), 264-272.

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Quantification of dsRNA in Cells

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After 24 h treatment, cells were collected by trypsinization and washed twice with PBS. Cells were fixed with 4% formaldehyde for 20 min at RT. After two washes with PBS, cells were permeabilized for 15 min at RT using 0.1% Triton X-100 in PBS. Cells then incubated with 1% BSA for at RT for 1 h followed by incubation with 2.5 μg/mL of anti-dsRNA (J2) antibody (CST, #76651) at RT for 1 h. After three washes cells were incubated with 2.2 μg/mL of Alexa Fluor 488 (AF488) conjugated goat anti-mouse IgG H&L antibody (abcam, #ab150113) at RT for 1 h. Cells then washed three times with PBS and suspended in 0.5% BSA, 2 mM EDTA in PBS. Centrifugation steps were performed at 300 × g in 4 °C. Cells were analyzed using MACSQuant analyzer (Miltenyi Biotec). Data were analyzed using FlowJo Software (10.9.0).

Wang Y., Pattarayan D., Huang H., Zhao Y., Li S., Wang Y., Zhang M., Li S, & Yang D. (2024). Systematic investigation of chemo-immunotherapy synergism to shift anti-PD-1 resistance in cancer. Nature Communications, 15, 3178.

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Isolation and Purification of Neuron Nuclei

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The isolated hippocampus tissue was transferred into a Dounce homogenizer (Sigma, D8938) containing 2ml of EZ Lysis Buffer (Sigma, NUC-101). The tissue was carefully dounced for 22 times with pestle A followed by 22 times with pestle B, then transferred to a 15ml-tube. Next, 1ml of EZ Lysis Buffer was added into the Dounce homogenizer to resuspend residual nuclei, then transferred to the same 15ml tube. The samples were centrifuged at 300g for 5 min.
Supernatant was removed and the pellet was resuspended in 100μl of ice-cold PBS (Gibco, 10010023) with 1% BSA (NEB, B9000S) and 20U RRI (Takara, 2313). 40μm FlowMi cell strainers were firstly wetted with PBS and filtered the resuspended nuclei into 1.5 ml Eppendorf tubes. The nuclei were further washed by PBS (1% BSA).
To enrich neuron nuclei, 1,000-fold diluted mouse Anti-NeuN antibody (Millipore, MAB377) was added into 0.5ml nuclei suspension and incubated with the nuclei at 4°C for 30min. The nuclei were then stained with 1000-fold diluted Goat anti-Mouse IgG (H&L) antibody (Abcam, ab150113) and washed with PBS (1% BSA). The whole process was on ice. As gated in Fig. S10B, single neuron nuclei were sorted with FACSAria SORP flow cytometer.
For frozen samples, hippocampus tissues were previously flash frozen in liquid nitrogen, and stored in -80°C. Before single nuclei preparation, they were thawed on ice totally.

Di L., Liu B., Lyu Y., Zhao S., Pang Y., Zhang C., Wang J., Qi H., Shen J., & Huang Y. (2021). Rapid and sensitive single cell RNA sequencing with SHERRY2.

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