Cd11b dcis1 18

Manufactured by Abcam

CD11b (DCIS1/18) is a mouse monoclonal antibody that recognizes the CD11b antigen, also known as the integrin alpha M chain or complement receptor 3 alpha subunit. CD11b is a cell surface glycoprotein that is expressed on the surface of various immune cells, including monocytes, macrophages, granulocytes, and natural killer cells. It plays a role in cell-cell and cell-extracellular matrix interactions, as well as in the phagocytosis of complement-opsonized particles.

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Lab products found in correlation

Cd19 sj25c1, by BD (2 mentions) Fcεri aer 37 cra 1, by BD (2 mentions) Cd45 hi30, by BD (1 mentions) Cd3 sk7, by BD (1 mentions) Cd123 fab301c, by R&D Systems (1 mentions) Cd11c bu15, by Abcam (1 mentions) Crth2 bm16, by Miltenyi Biotec (1 mentions) Il 7rα a019d5, by Thermo Fisher Scientific (1 mentions) Cd8 rpa t8, by BD (1 mentions) Cd45 h130, by BD (1 mentions) Live dead violet, by Thermo Fisher Scientific (1 mentions) Pd 1 eh12.2h7, by BD (1 mentions) Cd14 mφp9, by BD (1 mentions) Lsrfortessa, by BD (1 mentions)

2 protocols using cd11b dcis1 18

FACS Surface Staining of Immune Cells

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For FACS surface staining, the cells were labelled by the following anti-human antibodies: CD3 (SK7; BD), CD19 (SJ25C1; BD), CD123 (FAB301C; R&D Systems), CD11b (DCIS1/18), CD11c (BU15; Abcam), CD4 (A161A1, BD), CD8 (RPA-T8), FcεRI (AER-37 ([CRA-1]), CD14 (MϕP9; BD), CD45 (H130), CD56 (B159), CD45 (HI30, BD), CRTH2 (BM16; Miltenyi Biotec), IL-7Rα (A019D5), and live/dead violet (Invitrogen). The samples were acquired using FACSDiva on LSRFortessa flow cytometer. FlowJo software (FlowJo_v10.7.1) was used for data analysis.

Fonseka C.L., Hardman C.S., Woo J., Singh R., Nahler J., Yang J., Chen Y.L., Kamaladasa A., Silva T., Salimi M., Gray N., Dong T., Malavige G.N, & Ogg G.S. (2022). Dengue virus co-opts innate type 2 pathways to escape early control of viral replication. Communications Biology, 5, 735.

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Comprehensive Immune Cell Phenotyping

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The following anti human antibodies were purchased from Biolegend unless stated otherwise. CD19 (SJ25C1; BD Biosciences), CD11b (DCIS1/18; Abcam), CD11c (BU15), FcεRI (AER-37 (CRA-1)), CD14 (MφP9; BD Biosciences), TCRαβ (IP26), TCRγδ (B1), CD3 (SK7 and OKT3), CD45 (H130), CRTH2 (BM16; Miltenyi Biotec), CD127 (A019D5), c-Kit (104D2), CD44 (G45–26 and IM7), CCR7 (G043H7), PD-1 (EH12.2H7), CTLA-4 (L3D10 and BNI3 BD Biosciences), CD56 (HCD56 and 5.1H1L), CD69 (FN50), KLRG1 (SA231A2), MHC II (L243) and LIVE/DEAD Fixable Violet Dead Cell Stain Kit. Lineage stains were combined through a single channel, which allowed ILC phenotypic marker assessment but limited the potential to correlate with other cell populations.
Samples were acquired using FACSDiva software on LSR Fortessa (Becton Dickson). The flow cytometry data were analysed using FlowJo software.

Salimi M., Wang R., Yao X., Li X., Wang X., Hu Y., Chang X., Fan P., Dong T, & Ogg G. (2018). Activated innate lymphoid cell populations accumulate in human tumour tissues. BMC Cancer, 18, 341.

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