Anti p63 h 137

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-p63 (H-137) is a rabbit polyclonal antibody that recognizes the p63 protein. p63 is a member of the p53 family of transcription factors and plays a role in regulating cellular processes such as differentiation and development. The antibody can be used to detect p63 in various applications, including Western blotting, immunohistochemistry, and immunofluorescence.

Automatically generated - may contain errors

Lab products found in correlation

Anti p53 d0 1, by Santa Cruz Biotechnology (2 mentions) Anti p73, by Fortis Life Sciences (2 mentions) Anti flag, by Merck Group (1 mentions) Anti p53 do 1, by Santa Cruz Biotechnology (1 mentions) Lumi light western blotting substrate, by Roche (1 mentions) Abi prism 7500, by Thermo Fisher Scientific (1 mentions) Superscript vilo, by Thermo Fisher Scientific (1 mentions) Rabbit anti mouse igg, by Santa Cruz Biotechnology (1 mentions) Anti p63 4a4, by Santa Cruz Biotechnology (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Dnase 1, by Roche (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Sybr green pcr master mix, by Thermo Fisher Scientific (1 mentions) Anti h3k27ac, by Diagenode (1 mentions) Anti cebpa 14aa, by Santa Cruz Biotechnology (1 mentions) Enhanced chemiluminescence ecl western blotting system, by GE Healthcare (1 mentions) Sheep anti mouse igg, by GE Healthcare (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti ha high affinity, by Roche (1 mentions) Anti myc, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Anti-p63 (15 citations) H-137 (2 citations)

4 protocols using anti p63 h 137

Co-Immunoprecipitation of p53 and p63

Check if the same lab product or an alternative is used in the 5 most similar protocols

Co-Immunoprecipitation experiments were performed by lysing cells in RIPA buffer with 10% glycerol in order to stabilize protein-protein interactions. Protein complexes were then immunoprecipitated using appropriate antibodies. Complexes were analyzed by western blotting using appropriate antibodies, anti-p53 DO-1 (Santa Cruz Biotechnology), anti-p63 H-137 (Santa Cruz Biotechnology), and anti-FLAG (Sigma). Bound primary antibodies were visualized using Lumi-Light Western Blotting Substrate (Roche™) on a UVITEC Cambridge Camera.

Caratozzolo M.F., Marzano F., Abbrescia D.I., Mastropasqua F., Petruzzella V., Calabrò V., Pesole G., Sbisà E., Guerrini L, & Tullo A. (2019). TRIM8 Blunts the Pro-proliferative Action of ΔNp63α in a p53 Wild-Type Background. Frontiers in Oncology, 9, 1154.

+ Open protocol

+ Expand

RNA and Chromatin Immunoprecipitation Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

RNA was extracted in TRIzol reagent (Invitrogen) and treated with DNase I (Roche). Complementary DNA (cDNA) synthesis was obtained using SuperScript Vilo (Invitrogen). Expression of target genes was normalized for the β-actin gene expression. ChIP analysis was performed as previously described (43 (link)) using rabbit polyclonal antibody anti-p63 (H137, Santa Cruz Biotechnology), anti-histone H3K4me3 (Upstate 07–473), anti-H3K27Ac (Diagenode), anti-Cebpa (14AA, Santa Cruz Biotechnology), anti-Cebpb (C-19, Santa Cruz Biotechnology), anti- Pou3f1 (kindly provided by Dr. Michael Wegner, Universität Erlangen-Nürnberg, Germany) (55 (link)) or rabbit anti-mouse IgG (Santa Cruz Biotechnology). Real-time RT-PCR was performed using the SYBR Green PCR master mix (Applied Biosystems) in an ABI PRISM 7500 (Applied Biosystems). Oligonucleotide sequences are given in the Supplementary Data. For immunoblotting, cells were lysed in sample buffer and immunoblotted as previously described (43 (link)). The following primary antibodies were used: anti-p63 (4A4, Santa Cruz Biotechnology), anti-β-actin (Santa Cruz Biotechnology). For immunoblotting, secondary antibodies were sheep anti-mouse IgG conjugated to horseradish peroxidase (GE Healthcare) and visualized using Enhanced chemiluminescence (ECL) western blotting system (GE Healthcare).

Antonini D., Sirico A., Aberdam E., Ambrosio R., Campanile C., Fagoonee S., Altruda F., Aberdam D., Brissette J.L, & Missero C. (2015). A composite enhancer regulates p63 gene expression in epidermal morphogenesis and in keratinocyte differentiation by multiple mechanisms. Nucleic Acids Research, 43(2), 862-874.

+ Open protocol

+ Expand

Immunoprecipitation and ChIP Assays

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunoprecipitation from MDA-MB-231, A431 or H1299-R273H cytoplasmic extracts was performed as previously described.^{55 (link)} For each IP, 2.0 μg of anti-p53 (D0–1, Santa Cruz, Santa Cruz, CA, USA), anti-p63 (H-137, Santa Cruz), anti-p73 (Bethyl, Montgomery, TX, USA), anti-Ago2 (Mouse 2A8), rabbit IgG, mouse IgG and anti-HA beads was used. ChIP assays (Primer sequences in Supplementary Table S9) were performed as described previously.^{32 (link)}

Subramanian M., Francis P., Bilke S., Li X., Hara T., Lu X., Jones M., Walker R., Zhu Y., Pineda M., Lee C., Varanasi L., Yang Y., Martinez L., Luo J., Ambs S., Sharma S., Wakefield L., Meltzer P, & Lal A. (2014). A mutant p53/let-7i-axis-regulated gene network drives cell migration, invasion and metastasis. Oncogene, 34(9), 1094-1104.

+ Open protocol

+ Expand

Immunoblotting of Cell Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunoblotting was performed using standard protocols using anti-p53 (D0–1, Santa Cruz, 1:5000), anti-p63 (H-137, Santa Cruz, 1:2500), anti-p73 (Bethyl, 1:5000), anti-HA High affinity (Roche, Indianapolis, IN, USA, 1:1000), anti-Ago2 (2A8 1:5000), anti-CPSF1 (Sigma, 1:2500), anti-Myc (9E10 Santa Cruz, 1:2500) and anti-β-actin (Sigma, 1:5000) antibodies.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!