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Gm csf human simplestep elisa kit

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The GM-CSF Human SimpleStep ELISA kit is a sandwich enzyme-linked immunosorbent assay designed for the quantitative measurement of human Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) in cell culture supernatants, serum, and plasma samples.

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Human GM-CSF ELISA Kit SimpleStep ELISA Kit GM-CSF SimpleStep ELISA ELISAs

2 protocols using gm csf human simplestep elisa kit

1

Mechanical Memory Encodes Secretome Dynamics

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SUM159 cells were preconditioned for 7 days on stiff hydrogels to encode mechanical memory, and then 500,000 cells were transferred to 8.5 cm soft hydrogels. Media was changed the next day, and then 24 hours after that conditioned media (CM) was collected (2-day-soft CM), spun down to remove cells/debris and snap frozen. Cells were counted and then 500,000 cells were re-plated. This cycle was repeated to generate 4-, 6-, and 8-day soft CM. A GM-CSF Human SimpleStep ELISA kit was used per manufacturer’s instructions (Abcam). The colorimetric signal was normalized to total protein lysates from 25% of the cells on the hydrogels after each 2-day conditioning cycle (Coomassie Plus; Pierce). For mechanical memory selection, 100,000 CellVueHIGH or CellVueLOW cells were returned to 22 mm × 22 mm soft hydrogels for 24 hours in order to generate conditioned media (see Flow Cytometry and Sorting), and GM-CSF levels were interpolated from a standard curve per manufacturer’s instructions.
Watson A.W., Grant A.D., Parker S.S., Hill S., Whalen M.B., Chakrabarti J., Harman M.W., Roman M.R., Forte B.L., Gowan C.C., Castro-Portuguez R., Stolze L.K., Franck C., Cusanovich D.A., Zavros Y., Padi M., Romanoski C.E, & Mouneimne G. (2021). Breast tumor stiffness instructs bone metastasis via maintenance of mechanical conditioning. Cell reports, 35(13), 109293.
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2

Mechanical Memory Encodes Secretome Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols
SUM159 cells were preconditioned for 7 days on stiff hydrogels to encode mechanical memory, and then 500,000 cells were transferred to 8.5 cm soft hydrogels. Media was changed the next day, and then 24 hours after that conditioned media (CM) was collected (2-day-soft CM), spun down to remove cells/debris and snap frozen. Cells were counted and then 500,000 cells were re-plated. This cycle was repeated to generate 4-, 6-, and 8-day soft CM. A GM-CSF Human SimpleStep ELISA kit was used per manufacturer’s instructions (Abcam). The colorimetric signal was normalized to total protein lysates from 25% of the cells on the hydrogels after each 2-day conditioning cycle (Coomassie Plus; Pierce). For mechanical memory selection, 100,000 CellVueHIGH or CellVueLOW cells were returned to 22 mm × 22 mm soft hydrogels for 24 hours in order to generate conditioned media (see Flow Cytometry and Sorting), and GM-CSF levels were interpolated from a standard curve per manufacturer’s instructions.
Watson A.W., Grant A.D., Parker S.S., Hill S., Whalen M.B., Chakrabarti J., Harman M.W., Roman M.R., Forte B.L., Gowan C.C., Castro-Portuguez R., Stolze L.K., Franck C., Cusanovich D.A., Zavros Y., Padi M., Romanoski C.E, & Mouneimne G. (2021). Breast tumor stiffness instructs bone metastasis via maintenance of mechanical conditioning. Cell reports, 35(13), 109293.
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