1 2 distearoyl sn glycero 3 phosphoethanolamine

Fluorescence probes: N,N,N-Trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl) (DPH-TMA) was purchased from Sigma-Aldrich, USA.
Lipids: 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-diethylenetriaminepentaacetic acid (18:0 PE DTPA (Gd)), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1-stearoyl-2-linoleoyl-sn-glycero-3-phosphocholine (SOPC) 1,2-dioleoyl-sn-glycero-3-ethylphosphocholin (EPC 95%), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and cholesterol were purchased from Avanti lipids USA, with purity of 99%. All other chemicals were purchased from Sigma-Aldrich, USA.

Gentiopicroside was purchased from Innochem Technology Co., Ltd. (Beijing, China). Moreover, 1, 2-Distearoyl-sn-glycero-3-phosphoethanolamine (DSPE), cholesterol, and polyethylene glycol 2000 (PEG2000) were purchased from Sigma-Aldrich, (St. Louis, MO, USA). Chitosan was prepared in our laboratory. IMQ cream and tacrolimus ointment were obtained from Sichuan Mingxin Pharmaceutical Co., Ltd. (Chengdu, China) M5 was purchased from Nearshore Proteins. BALB/c mice and HaCaT cells were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China) and the Cell Resource Center at the Institute of Basic Medicine of the Chinese Academy of Medical Sciences (Beijing, China).
Dulbecco’s modified eagle medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco (Waltham, MA, USA). The annexin V-FITC/propidium iodide (PI) apoptosis assay kit, penicillin-streptomycin mixture, and trypsin-EDTA solution (TRY) were purchased from Solarbio Life Sciences (Beijing, China). In addition, 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium ammonium bromide (MTT) was purchased from Beijing Okos Technology Co., Ltd. (Beijing, China). Other reagents were purchased from Innochem Technology Co., Ltd.

Resveratrol (C14H12O3; > 98% pure) was purchased from Shaanxi Sciphar Biotechnology Co., Ltd. 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine and polyethylene glycol were purchased from Sigma Inc. Res-NPs were constructed as described in our previous study [12 (link)]. Phosphate-buffered saline (PBS), saline, apoptosis detection kit (Annexin VFITC/PI), protein extraction kit, malondialdehyde (MDA) kit, glutathione peroxidase (GSH-Px) kit, terminal deoxynucleotidyl transferase-mediated deoxyuridine-triphosphate nick end-labeling (TUNEL) apoptotic detection kit, and primary antibodies (BAX, caspase3, and BCL-2) were all purchased from Nanjing Key Gen Biotech. Co., Ltd. Blood urea nitrogen (BUN), C-reactive protein (CRP), cystatin C protease inhibitors (Cys-C), and serum creatinine (SCr) were all obtained from Weiteman, Co., Ltd., (Nanjing, China).
The following instruments were used: automatic biochemical analyzer (Hitachi 7100, Japan), microplate reader (ELx800, BioTek, USA), flow cytometer (FACSCalibur, Becton-Dickinson, USA), inverted fluorescence microscope (IX51, Olympus, Japan), Western electrophoresis apparatus (164-5051, Bio-Rad, USA), spectrophotometer (UV-2540, Shimadzu, Japan), and gel imager (Gel Doc XR, Bio-Rad, USA). Primary antibodies (IGFBP2, AAab136494; KIM-1, ab47634; NGAL, and ab63929) were purchased from Abcam (Cambridge, UK).

Egg yolk phosphatidylcholine (PC), phosphatidylglycerol (PG), cholesterol (Chol), 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine (PEG) and Paclitaxel were purchased from Sigma (St Quentin-Fallavier, France). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000 (Mal-PEG were purchased from Coger (Paris, France). Docetaxel was purchased from VWR (Fontenay sous bois, France) and DiD fluorescent tag from Thermo Fisher (Illkirch, France). Trastuzumab (Herceptin) was kindly given by Genentech (South San Francisco, CA, USA). Anti-human IgG PE (clone L1C1) and a prototyped human IgG kappa light chains quantification kit for submicron particles (e.g., immunoliposomes) were provided by Biocytex (Marseille, France) as described below. All other reagents were of analytical grade.