Through immunohistochemical staining (IHC), the level of SOX6 protein was detected in tissue microarray (No. RTCEC‐201704100) purchased from Raisedragon's Co., Ltd. (Beijing, China). In brief, the level of SOX6 protein was detected by rabbit anti‐SOX6 (1:500, Abcam, UK, #ab30455), according to the protocol of MaxVision TM HRP‐Polymer anti‐Rabbit IHC Kit (Maixin, Fuzhou, China).
Maxvision tm hrp polymer anti rabbit ihc kit
Manufactured by Maixin Group
Sourced in China
The MaxVision™ HRP-Polymer anti-Rabbit IHC Kit is a lab equipment product designed for immunohistochemistry (IHC) applications. It utilizes a horseradish peroxidase (HRP)-based detection system to visualize target antigens in tissue samples when using rabbit-derived primary antibodies.
Automatically generated - may contain errors
Lab products found in correlation
Ab28699, by Abcam (1 mentions)
Dab detection kit, by Maixin Group (1 mentions)
Rabbit anti hbc, by Agilent Technologies (1 mentions)
Anti pd l1 antibody, by Cell Signaling Technology (1 mentions)
Dab horseradish peroxidase color development kit, by Maixin Group (1 mentions)
Ab125066, by Abcam (1 mentions)
Ab150077, by Abcam (1 mentions)
Hematoxylin, by Merck Group (1 mentions)
3 3 diaminobenzidine (dab), by Maixin Group (1 mentions)
Dm4000b, by Leica camera (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Tunel assay kit, by Roche (1 mentions)
6 protocols using maxvision tm hrp polymer anti rabbit ihc kit
1
SOX6 Protein Expression in Tissue
2
Immunohistochemical Analysis of HOXA11 in Lung Adenocarcinoma
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Paraffin-embedded TMA blocks, including 160 lung adenocarcinoma samples (40 lung AIS, 40 lung AD and 80 normal counterparts), were sectioned at a 4 μm thickness, deparaffinized in xylene, and rehydrated in graded ethanol solutions, and the endogenous peroxidase activity was blocked by incubation with 3% H2O2 for 30 min at room temperature. Then, the sections were immersed in a citrate-NaOH buffer (10 mM sodium citrate, pH 7.0) for 40 min at 92°C to restore antigenicity. The rehydrated sections were incubated overnight at 4°C with the rabbit anti-human HOXA11 polyclonal antibody (1:250, ab28699, Abcam, USA.). The sections incubated with the first antibody were washed with Tris-buffered saline (TBS) and were then incubated with the MaxVision TM HRP-Polymer anti-Rabbit IHC Kit (Maixin, Fuzhou, China) for 15 min at room temperature. The sections were visualized using the DAB Detection Kit (Maixin, Fuzhou, China), and the reaction was followed by counterstaining with hematoxylin. The negative control experiments were performed by omitting the primary antibody.
3
Hepatitis B Virus Immunohistochemistry
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The mice were sacrificed at 72 hours post-injection, and the liver was removed and fixed in formalin (3.7% Formaldehyde in PBS) for immunohistochemistry analysis, HBcAg was detected by immunohistochemical staining by rabbit anti-HBc (DAKO, Carpinteria, CA; Biomeda, Foster City, CA) and MaxVision TM HRP-Polymer anti-Rabbit IHC Kit (Maixin, Fuzhou, China).
4
Immunohistochemical Analysis of Tumor Samples
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All of the 120 patient tumor tissue samples were analyzed immunohistochemically. Immunohistochemistry (IHC) was performed on 5 mm sections of formalin-fixed, paraffin-embedded tissue samples. The paraffin sections were deparaffinized with xylene and rehydrated in alcohol. Antigen retrieval was accomplished by boiling citrate buffer and endogenous peroxidase activity was blocked with 3% H2O2 followed by staining with anti-PD-L1 antibody (1:100; Cell Signaling Technology, Danvers, MA, USA), anti-CD4 antibody (1:500; ZSGB-BIO, Beijing, China) or anti-CD8 antibody (1:500; ZSGB-BIO, Beijing, China) overnight at 4 °C. After washing, the sections were processed with a MaxVisionTM HRP-Polymer anti-Rabbit IHC Kit at room temperature (Maixin, Fuzhou, China) and then developed with a DAB Horseradish Peroxidase Color Development Kit (Maixin, Fuzhou, China) and counterstained with hematoxylin.
5
Immunohistochemical and Immunofluorescent Tissue Staining
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Slides containing tissues were immersed in xylene followed by decreasing concentrations of alcohol (100%, 95%, 90%, 85%, and 75%) and finally phosphate buffer solution (PBS). The slides were incubated in citrate buffer to retrieve the epitope for 20 min at 95 °C, treated with 3% hydrogen peroxide for 10 min, and then exposed to 10% goat serum for 60 min at room temperature.
For immunohistochemical staining, primary antibodies (HAVCR1 (KIM-1), Proteintech, 30948) were added overnight at 4 °C.slides were washed with PBS for immunohistochemical staining and subsequently treated with the MaxVision TM HRP-Polymer anti-Rabbit IHC Kit (MaixinBio, KIT-5004) for 15 min at room temperature, then washed with PBS. Sections were counterstained with DAB for 10 min then washed with PBS. After treatment with hematoxylin for five minutes and washing with PBS, microscope photographs were taken.
For immunofluorescent staining, primary antibodies (GPX4, ab125066, Abcam; SLC7A11, 11549-1-AP, Proteintech; p-Camkk2, 12818, CST; p-AMPK, 2535, CST) were added overnight at 4 °C.
Afterwards, all slides were washed with PBS and treated with AF488-conjugated anti-rabbit antibody (ab150077, Abcam) for 60 min at room temperature, followed by additional washing with PBS. Finally, the sections were counterstained with DAPI and photographed using a fluorescence microscope.
For immunohistochemical staining, primary antibodies (HAVCR1 (KIM-1), Proteintech, 30948) were added overnight at 4 °C.slides were washed with PBS for immunohistochemical staining and subsequently treated with the MaxVision TM HRP-Polymer anti-Rabbit IHC Kit (MaixinBio, KIT-5004) for 15 min at room temperature, then washed with PBS. Sections were counterstained with DAB for 10 min then washed with PBS. After treatment with hematoxylin for five minutes and washing with PBS, microscope photographs were taken.
For immunofluorescent staining, primary antibodies (GPX4, ab125066, Abcam; SLC7A11, 11549-1-AP, Proteintech; p-Camkk2, 12818, CST; p-AMPK, 2535, CST) were added overnight at 4 °C.
Afterwards, all slides were washed with PBS and treated with AF488-conjugated anti-rabbit antibody (ab150077, Abcam) for 60 min at room temperature, followed by additional washing with PBS. Finally, the sections were counterstained with DAPI and photographed using a fluorescence microscope.
6
LPAR1 Immunohistochemistry Protocol
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LF tissue from patients or rats was fixed with 4% paraformaldehyde 48 h after surgery. Sections of LF (5 μm thick) were obtained to prepare slides. The immunohistochemistry staining procedures for LPAR1 were performed on the slides. The slides were de-paraffinized in graded xylene and rehydrated in graded ethanol solutions. The slides were then incubated in retrieval buffer (Roche) at 37°C for 30 min to retrieve antigen. Endogenous peroxidase activity was blocked in H 2 O 2 solution (3% H 2 O 2 in PBS buffer) at RT for 30 min. The slices were incubated in blocking buffer at RT for 30 min and subsequently incubated with a primary antibody at 4°C overnight. The primary antibody included anti-LPAR1 (CST 1:100), anti-PCNA (CST 1:100), anti-p-Akt (CST 1:100), anti-cleaved Caspase 3 (CST 1:100), and anti-BAX (CST 1:100). Next, the secondary antibody (MaxVision TM HRP-polymer anti-rabbit IHC kit, Maixin-Bio, China) was added for 15 min at RT and DAB (Maixin-Bio) solution was applied as the chromogen. Finally, hematoxylin (Sigma-Aldrich) was used to counterstain the slices to identify nuclei. A TUNEL assay kit (Roche) was also used to test for apoptosis. DAPI (Sigma-Aldrich) was used according to the manufacturer's protocol to identify nuclei. The images were observed and analyzed using a microscope (Leica DM 4000B) and analyzed with IPP6.0.
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