Log In Sign up
The largest database of trusted experimental protocols

Rabbit anti phospho plcγ1 tyr783 antibody

Manufactured by Cell Signaling Technology
Visit Supplier

Rabbit anti-phospho-PLCγ1 (Tyr783) antibody is a research-use antibody that detects the phosphorylation of phospholipase C gamma 1 (PLCγ1) at tyrosine 783. This antibody can be used in techniques such as Western blotting to study the activation and regulation of PLCγ1, which plays a role in various cellular signaling pathways.

Automatically generated - may contain errors

Lab products found in correlation

Nitrocellulose membrane, by Bio-Rad (2 mentions) Af3057, by R&D Systems (2 mentions) Ab10983, by Abcam (2 mentions) Ab135711, by Abcam (2 mentions) Anti p44 42 mapk erk1 2 137f5 rabbit mab, by Cell Signaling Technology (2 mentions) Rabbit anti plcγ1 d9h10 xp rabbit mab, by Cell Signaling Technology (2 mentions) Phospho p44 42 mapk erk1 2 thr202 tyr204, by Cell Signaling Technology (2 mentions)

Spelling variants of this product

PLCγ1 (37 citations) Anti-PLCγ1 (19 citations) Phospho-PLCγ1 (10 citations) Anti-phospho-PLCγ1 (Tyr783) (9 citations) Anti-phospho-PLCγ1 (6 citations) Rabbit-anti-phospho-PLCγ1 (3 citations) Rabbit anti-PLCγ1 (2 citations) PLCγ1 Antibody (2 citations)

2 protocols using rabbit anti phospho plcγ1 tyr783 antibody

1

Western Blot Analysis of Thermogenic Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells or tissues were lysed by using Nonidet P-40 lysis buffer containing 50 mmol/liter Tris (pH 7.5), 150 mmol/liter NaCl, 10% glycerol, 0.5% Nonidet P-40, and protease inhibitors. The supernatant was used for protein quantification using BCA protein assay kits (23227, Pierce). Proteins were resolved by SDS–PAGE and transferred to nitrocellulose membranes (Bio-Rad).
Antibodies used: Rabbit polyclonal anti-UCP1 antibody (ab10983, Abcam), rabbit polyclonal anti-DIO2 (ab135711, Abcam), polyclonal goat anti-FGF21 (AF3057, R&D), rabbit polyclonal anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (#9101, Cell Signaling), anti-p44/42 MAPK (Erk1/2) (137F5) rabbit mAb (#4695, Cell Signaling), rabbit anti-phospho-PLCγ1 (Tyr783) antibody (#2821, Cell Signaling), and rabbit anti-PLCγ1 (D9H10) XP Rabbit mAb (#5690, Cell Signaling).
Abu-Odeh M., Zhang Y., Reilly S.M., Ebadat N., Keinan O., Valentine J.M., Hafezi-Bakhtiari M., Ashayer H., Mamoun L., Zhou X., Zhang J., Yu R.T., Dai Y., Liddle C., Downes M., Evans R.M., Kliewer S.A., Mangelsdorf D.J, & Saltiel A.R. (2021). FGF21 promotes thermogenic gene expression as an autocrine factor in adipocytes. Cell reports, 35(13), 109331.
+ Open protocol
+ Expand
2

Western Blot Analysis of Thermogenic Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells or tissues were lysed by using Nonidet P-40 lysis buffer containing 50 mmol/liter Tris (pH 7.5), 150 mmol/liter NaCl, 10% glycerol, 0.5% Nonidet P-40, and protease inhibitors. The supernatant was used for protein quantification using BCA protein assay kits (23227, Pierce). Proteins were resolved by SDS–PAGE and transferred to nitrocellulose membranes (Bio-Rad).
Antibodies used: Rabbit polyclonal anti-UCP1 antibody (ab10983, Abcam), rabbit polyclonal anti-DIO2 (ab135711, Abcam), polyclonal goat anti-FGF21 (AF3057, R&D), rabbit polyclonal anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (#9101, Cell Signaling), anti-p44/42 MAPK (Erk1/2) (137F5) rabbit mAb (#4695, Cell Signaling), rabbit anti-phospho-PLCγ1 (Tyr783) antibody (#2821, Cell Signaling), and rabbit anti-PLCγ1 (D9H10) XP Rabbit mAb (#5690, Cell Signaling).
Abu-Odeh M., Zhang Y., Reilly S.M., Ebadat N., Keinan O., Valentine J.M., Hafezi-Bakhtiari M., Ashayer H., Mamoun L., Zhou X., Zhang J., Yu R.T., Dai Y., Liddle C., Downes M., Evans R.M., Kliewer S.A., Mangelsdorf D.J, & Saltiel A.R. (2021). FGF21 promotes thermogenic gene expression as an autocrine factor in adipocytes. Cell reports, 35(13), 109331.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!