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Ly5mouse b6 sjl ptprcapepcb boycrl

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The Ly5Mouse/B6.SJL-PtprcaPepcb/BoyCrl is a laboratory mouse strain. It is a congenic strain that expresses the CD45.1 (Ly5.1) allele on the C57BL/6 (B6) background. This strain is commonly used as a donor strain in hematopoietic cell transplantation studies.

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Lab products found in correlation

Il7r mice, by Jackson ImmunoResearch (2 mentions) Cd45.1 wt mice, by Charles River Laboratories (1 mentions)

2 protocols using ly5mouse b6 sjl ptprcapepcb boycrl

1

Adoptive Transfer and Reconstitution Assays

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C57Bl/6 WT mice, CD45.1+ WT mice (Charles River, Sherbrooke, QC, Canada: Ly5Mouse/B6.SJL-PtprcaPepcb/BoyCrl) and Il7rtm1Imx/Il7rtm1Imx CD45.2+ mice (referred to as Il7r−/− mice, Jackson Laboratories, Bar Harbor, MA, USA) were maintained at the Sunnybrook Research Institute (SRI), Toronto, ON, Canada. All animal protocols were approved by the SRI animal care committee. Mice aged 4.5–6 weeks old were used for ex vivo analysis of T cells and thymic DCs. F1 progeny of CD45.2+ C57Bl/6 and CD45.1+ C57Bl/6 parents, aged 4.5–6 weeks old, were used as recipients for BM chimera and competitive BM reconstitution experiments. Mice aged 7–12 weeks old were used for BM donor cells. Donor mice were age-matched in each independent experiment, as were recipient mice.
Moore A.J., In T.S., Trotman-Grant A., Yoganathan K., Montpellier B., Guidos C.J., Zúñiga-Pflücker J.C, & Anderson M.K. (2017). A key role for IL-7R in the generation of microenvironments required for thymic dendritic cells. Immunology and Cell Biology, 95(10), 933-942.
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2

T Cell Development in Mouse Models

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C57Bl/6 wildtype (WT) mice, CD45.1+ WT mice (Charles River, Charles River: Ly5Mouse/B6.SJL-PtprcaPepcb/BoyCrl) and Il7rtm1Imx/Il7rtm1Imx CD45.2+ mice (referred to as Il7r−/− mice, Jackson Laboratories) were maintained at the Sunnybrook Research Institute (SRI), Toronto, Ontario, Canada. All animal protocols were approved by the SRI animal care committee. Mice aged 4.5–6 weeks old were used for ex vivo analysis of T cells and thymic DCs. F1 progeny of CD45.2+ C57Bl/6 and CD45.1+ C57Bl/6 parents, aged 4.5–6 weeks old, were used as recipients for bone marrow (BM) chimera and competitive BM reconstitution experiments. Mice aged 7–12 weeks old were used for bone marrow donor cells. Donor mice were aged-matched in each independent experiment, as were recipient mice.
Moore A.J., In T.S., Trotman-Grant A., Yoganathan K., Montpellier B., Guidos C.J., Zúñiga-Pflücker J.C, & Anderson M.K. (2017). A key role for IL-7R in the generation of microenvironments required for thymic dendritic cells. Immunology and Cell Biology, 95(10), 933-942.
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