Fitc conjugated anti cd25 clone 7d4

Manufactured by BD

FITC-conjugated anti-CD25 (clone 7d4) is a monoclonal antibody that binds to the CD25 antigen. CD25 is the alpha chain of the interleukin-2 receptor and is expressed on activated T cells and regulatory T cells. This product is conjugated with the fluorescent dye FITC (Fluorescein Isothiocyanate).

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Lab products found in correlation

Facscanto, by BD (1 mentions) Mouse regulatory staining kit, by Thermo Fisher Scientific (1 mentions) Gentlemacs, by Miltenyi Biotec (1 mentions) Facscan, by BD (1 mentions)

Close spelling variants of this product

CD25-FITC (46 citations) Anti-CD25-FITC (29 citations) FITC-conjugated anti-CD25 (12 citations) FITC-conjugated CD25 (6 citations) CD25 (7D4, (5 citations) Anti-CD25 FITC (7D4) (3 citations) Anti-CD25-FITC (clone 7D4, (2 citations) Anti-CD25 (clone 7D4; (2 citations)

2 protocols using fitc conjugated anti cd25 clone 7d4

Profiling Immune Infiltration in Renal Tumors

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To characterize the infiltration of renal tumors by immune cells, kidneys were removed, crushed using Gentle Macs (Miltenyi) to prepare cell suspensions, and subsequently stained for 30 min at 4°C with the following monoclonal antibodies : PE-Cy7-conjugated anti-CD45 (clone 30-F11), PerCP-Cy5.5-conjugated anti-CD19 (clone 1D3), APC-conjugated anti-GR1 (clone RB6-8C5), FITC-conjugated anti-CD11b (clone M1/70), APC-Cy7-conjugated anti-CD3 (clone 145-2C11), APC-conjugated anti-CD4⁺ (clone RM4-5), PerCP-conjugated anti-CD8⁺ (clone 53-6.7) and FITC-conjugated anti-CD25 (clone 7d4), all from BD Biosciences. Regulatory T cells (Treg) were identified with the Mouse Regulatory Staining kit (eBiosciences). FACS^® Canto (BD Biosciences) was used for flow cytometry acquisition and analyses were performed with the FACS^® Diva software.

Fend L., Remy-Ziller C., Foloppe J., Kempf J., Cochin S., Barraud L., Accart N., Erbs P., Fournel S, & Préville X. (2015). Oncolytic virotherapy with an armed vaccinia virus in an orthotopic model of renal carcinoma is associated with modification of the tumor microenvironment. Oncoimmunology, 5(2), e1080414.

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Lymphocyte Depletion Assay with Anti-CD25 and Anti-NK1.1

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Target cell depletion following anti-CD25 mAb (PC61) or anti-NK1.1mAb (PK136) was assessed in peripheral blood and lymph node compartments. In brief, peripheral blood single suspensions and lymph node single suspensions were prepared. Cells were incubated for 30 min at 4°C. NK cells were stained using PE-conjugated anti-pan-NK (clone DX5) (BD Pharmingen). The cells were washed with FACS medium twice and fixed in 2% paraformaldehyde solution. Lymphocytes were stained in the same manner using FITC-conjugated anti-CD25 (clone 7D4) (BD Bioscience), PE conjugated anti-CD4 (clone RM4-5) (BD Bioscience), Cy-5 conjugated anti-CD4 (cloneGK1.5) (eBioscience) and Cy-conjugated anti-CD3e (clone 2C11) (BD Bioscience). To examine the levels of Foxp3⁺ T cells, PE-conjugated anti-Foxp3 intracellular staining (clone FJK-16s) (eBioscience) was performed. Lymphocytes were analyzed on a FACScan (BD Bioscience) using two or three color FACSCaliber analysis.

Hirohashi T., Chase C.M., DellaPelle P., Sebastian D., Farkesh E., Colvin R.B., Russell P.S., Alessandrini A, & Madsen J.C. (2014). Depletion of T Regulatory Cells Promotes Natural Killer Cell-Mediated Cardiac Allograft Vasculopathy. Transplantation, 98(8), 828-834.

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