Tsq8000 ms detector

The assay was carried out using a Trace 1310 series GC with a TSQ8000 MS detector (Thermo Fisher Scientific Co. Ltd., Waltham, Massachusetts, MA, USA). A 1 μL portion of extract was injected in splitless mode onto the column. Chromatographic separation was performed on a TR-5ms capillary column (30 m × 0.25 mm ID; DF = 0.25 μm; Thermo Fisher Scientific Co. Ltd., Waltham, MA, USA). Helium was used as the carrier gas at a constant flow rate of 1 mL/min through the column. The injector temperature was set at 280 °C and the injector temperature was 280 °C. The oven program was as follows: 50 °C for 2 min, linear ramp at a rate of 20 °C·min⁻¹ to 200 °C, followed with a linear ramp at a rate of 5 °C·min⁻¹ to 300 °C, held at 300 °C for 10 min. The transfer line temperature was 280 °C. The data was acquired and processed using Thermo Scientific Xcalibur data handling software.

The assay was carried out using a Trace 1310 series GC with a TSQ8000 MS detector (Thermo Fisher Scientific, United States). A TR-5 ms capillary column (30 m × 0.25 mm i.d., 0.25 μm film thickness; Thermo Fisher Scientific, United States) was used. The carrier gas for GC was helium at a flow rate of 1.0 mL⋅min^–1. The oven program was as follows: 50°C for 2 min, linear ramp up at a rate of 5°C⋅min^–1 to 230°C, held at 230°C for 5 min, followed with a linear ramp up at a rate of 10°C⋅min^–1 to 300°C, held at 300°C for 2 min. The injector temperature and transfer line temperature were 280°C.
A chiral column, Agilent CycloSil-B (30 m × 0.25 mm i.d., 0.25 μm film thickness), was used to identify the chirality of the assay product and the content of borneol and camphor in C. camphora leaves. The carrier gas for GC was helium at a flow rate of 1.0 mL⋅min^–1. The oven program was as follows: 50°C for 2 min, followed by a gradient from 50°C to 180°C at 5°C⋅min^–1, then 10°C⋅min^–1 to 230°C, held at 230°C for 2 min. The injector temperature was 200°C, and the transfer line temperature was 230°C.