Quantichrom creatinine assay kit dict 500

Fresh mouse blood samples were centrifuged at 2500 rpm at 4° for 10 min. Serum creatinine levels were detected using the Quantichrom Creatinine Assay Kit (DICT‐500; BioAssay Systems) as the manufactural instructions.

Creatinine levels were measured in serum by a quantitative colorimetric method using a QuantiChrom Creatinine Assay Kit (DICT-500), supplied by BioAssay Systems, Hayward, CA, USA, following the manufacturer’s instructions and according to the modified Jaffe method [18 (link)].Urea levels were measured in serum by a quantitative colorimetric method according to [20 (link)] using a QuantiChrom Urea Assay Kit (DIUR-500), supplied by BioAssay Systems, Hayward, CA, USA, according to the manufacturer’s instructions.

Timed (12-hour) urine collections were obtained from mice using metabolic cages. Urine protein was measured by an ELISA kit (Bethyl Laboratory, Houston, TX) for albumin, and urine creatinine levels were quantified using a QuantiChrom Creatinine Assay Kit (DICT-500; Bioassay Systems). Urine albumin excretion was expressed as the UACR. As for the reproducibility of this assay, the coefficients of variance (CV) was less than 3% when the same sample was measured three times consecutively.

Urine samples were collected 2.5–4 h after the end of cardiac bypass and immediately frozen (−20°C) before subsampling and refrozen for long-term conservation (−80°C). Urinary peptidome analysis was performed as previously described [8 (link)]. For urinary neutrophil gelatinase-associated lipocalin (uNGAL) measurement, urine samples were centrifuged for 10 min at 2500 rpm. NGAL was measured using an enzyme-linked immunosorbent assay (Human Lipocalin-2/NGAL Duoset ELISA; DY1757; R&D Systems, Minneapolis, MN, USA) in diluted supernatant (1:10 or 1:100) according to the manufacturer's protocol. Creatinine was measured using the QuantiChrom Creatinine Assay Kit (DICT 500; BioAssay Systems, Hayward, CA, USA) according to the manufacturer's protocol. Creatinine-normalized uNGAL (μg/g) was used for performance evaluations. [TIMP-2] and [IGFBP7] were measured in urine supernatants using the VITROS NephroCheck immunoassay on a VITROS 5600 Integrated System (Ortho Clinical Diagnostics, Raritan, NJ, USA) according to the manufacturer's instructions. The VITROS NephroCheck test result is a single number, which is a product of the measured concentrations of the two analytes in the sample divided by 1000. Certified laboratory technicians blinded to clinical data performed the analyses.