R6G-loaded PUs assemblies were incubated in a 10 mM GSH solution, recording the fluorescence emission spectra overtime at λex of 526 nm on an F-4600 FL spectrophotometer (Hitachi, Ltd., Japan). The FL intensity recover rate was calculated by (I − I0)/(Iw − I0) × 100% as a function of time, where I is the FL intensity of R6G at different times, I0 is the FL intensity at initial time, Iw is the FL intensity of free fluorescent probe dissolved in water with the same concentration as that encapsulated in assembled solutions. For FRET measurement, QD-loaded PUs assemblies were treated with 10 mM GSH and measured with an F-4600 FL spectrophotometer (Hitachi, Ltd., Japan) at different time points. The emission spectra were collected from 440 to 700 nm at a λex of 430 nm.
F 4600 fl spectrophotometer
Manufactured by Hitachi
Sourced in Japan
The F-4600 FL spectrophotometer is a high-performance instrument designed for fluorescence analysis. It is capable of measuring the intensity of fluorescent light emitted by a sample when exposed to an excitation light source. The F-4600 FL spectrophotometer is a versatile tool that can be used for a wide range of applications, including biochemical analysis, materials science, and environmental monitoring.
Automatically generated - may contain errors
11 protocols using f 4600 fl spectrophotometer
1
Fluorescence Quenching and FRET Analysis
2
Fluorescence Spectra of PUs in DMF-CHCl3
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Fluorescence spectra (λex = 365 nm) of PUs dispersed in mixtures of DMF-CHCl3 (1 mg mL−1) with different CHCl3 fractions (fw) were recorded on an F-4600 FL spectrophotometer (Hitachi, Ltd., Japan) at 25 °C, with an emission slit width of 5 nm and constant scan rate. The emission images of the mixture solutions were captured on a Leica microsystem (DMi 8).
3
Comprehensive Analytical Characterization of Compound
4
Synthesis and Characterization of Fluorescent Compounds
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Commercially available reagents were used without further purification. The solvents used for experiment research were all through pretreatment on condition of anaerobic and without water. Reactions were monitored by TLC using Silicycle precoated silica gel plates. Flash column chromatography was performed over Silicycle silica gel (300–400 mesh). 1H NMR and 13C NMR spectra were recorded on JMTC-400/54/SS 400 MHz spectrometers using residue solvent peaks as internal standards (CHCl3, 1H: 7.26 ppm; 13C: 77.00 ppm). The fluorescence spectra of samples were detected with a Fluorescence spectrophotometer (F-4600FL Spectrophotometer, Hitachi, Japan) using a Xenon lamp as the excitation source at room temperature, and the excitation wavelength was 331 nm, 228 nm and 237 nm.
5
Adamantoylation and Fluorescence of Spinach RNA
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This study was performed by using long RNA aptamers. The adamantoylation of Spinach RNA was performed using the above general protocol. For aptamer folding and fluorescence study, the Spinach RNA at a final concentration of 0.5 μM was incubated with 10 μM DFHBI in 150 μl folding buffer (40 mM HEPES at pH 7.4, 100 mM KCl, 5 mM MgCl2). After 1 h dark incubation at room temperature, the emission spectrum was collected in wavelength range 460–600 nm. The excitation wavelength was set to 450 nm and a 1 cm path length cell is used. Fluorescence detection was performed at room temperature using a F-4600 FL Spectrophotometer (Hitachi). Slit width: excitation = 5 nm; emission = 5 nm.
6
In Vitro Kinetic Assay for LbuCas13a
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This assay was performed with 45 nM purified LbuCas13a, 22.5 nM crRNA13a, 22.5 nM target RNA, 125 nM quenched fluorescent RNA reporter (reporter1 in Supplementary Table 1 ) and 0.5 U RiboLock RNase inhibitor (Thermo Fisher Scientific), in 1× Cas13a buffer10 (link). The curve describing fluorescence versus time was determined using an F-4600 FL Spectrophotometer (Hitachi) equipped with a xenon lamp with the kinetics mode at room temperature. For kinetic measurement, RNA reporter at a final concentration of 125 nM was added at time (t) = 0. Reactions were allowed to proceed for 1 hr measured every 1 s. The excitation and emission wavelengths are set to 496 and 520 nm and a 1-cm path-length cell is used. Slit width: excitation = 10 nm; emission = 10 nm. The gRNA without any treatment is used as an internal control.
7
Fluorescent Assay of PU Assemblies
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ThT (0.1 mg mL−1) was added to PU assemblies (1 mg mL−1) and the fluorescence measurement was conducted on an F-4600 FL spectrophotometer (Hitachi, Ltd., Japan) with a bandwidth of 5 nm and λex of 440 nm.
8
Characterization of Activated Sludge EPS
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A heat extraction methods was used to extract the EPS of activated sludge [27] (link). The concentrations of protein and carbohydrate in the EPS extraction were respectively measured by the modified Lowry method and phenol-sulphuric acid method [28] (link). At the end of the batch experiments, the EPS was extracted and measured using EEM fluorescence spectroscopy to characterize the structure of EPS with luminescence spectrometry (F-4600 FL Spectrophotometer, Hitachi, Japan) [29] (link). Particulates in samples were removed using a 0.45 μm polytetrafluoroethylene (PTFE) membrane prior to EEM tests. To obtain the fluorescence of EEM, The excitation and emission slits were set to a 5-nm band-pass and the scanning speed was 1200 nm/min. The excitation and emission wavelengths were incrementally increased from 200 to 450 nm and 200 to 550 nm at 5 nm and 0.5 nm steps, respectively.
9
Synthesis and Characterization of Luminescent IMS1 Ligand Complex
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All reagents and chemicals are purchased from HEOWNS, which are used without further purification. The 1,3-bis(4-carboxylbenzyl)-imidazolium (IMS1) ligand was synthesized according to the previously reported method.14 (link) Elemental analyses (C, H, N) were carried out using a Perkin-Elmer 240C elemental analyser. Infrared spectra were recorded in the range of 4000–400 cm−1 on a Nicolet 380 FT-IR spectrometer with KBr Pellets. Powder X-ray diffraction (PXRD) were measured at room temperature using a Rigaku Ultima IV diffractometer with graphite monochromatic Cu Kα radiation (λ = 0.15418 nm) at 40 kV and 40 mA. Simulated powder patterns were based on single crystal data and calculated using the Mercury software.15 (link) Thermogravimetric analysis (TGA) was performed using a STA 409 PC thermal analyser under the nitrogen atmosphere with a heating rate of 10 °C min−1. The luminescent spectra for complex 1 were recorded at ambient temperature using a Hitachi F-4600 FL spectrophotometer. UV/Vis spectra were obtained using a Hitachi UH4150 Spectrophotometer within 300–800 nm.
10
Biodistribution of Fluorescent Nanoparticles
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Free Rhodamine B, Rhodamine B-labeled PEI–PLGA nanoparticles,
and Rhodamine B-labeled PEG–PLGA nanoparticles were intravenously
injected into BALB/c mice (n = 3). The fluorescence
intensities (552 nm excitation and 588 nm emission) of the three formulations
were adjusted to be the same on a spectrophotometer (F-4600 FL spectrophotometer,
Hitachi, Japan). All the mice were sacrificed at 8 h post-injection.
Submandibular salivary glands and other major organs, including liver,
kidney, spleen, lung, heart, thymus gland, and brain, were dissected
and imaged using a fluorescence imaging system (Spectrum CT, PerkinElmer).
and Rhodamine B-labeled PEG–PLGA nanoparticles were intravenously
injected into BALB/c mice (n = 3). The fluorescence
intensities (552 nm excitation and 588 nm emission) of the three formulations
were adjusted to be the same on a spectrophotometer (F-4600 FL spectrophotometer,
Hitachi, Japan). All the mice were sacrificed at 8 h post-injection.
Submandibular salivary glands and other major organs, including liver,
kidney, spleen, lung, heart, thymus gland, and brain, were dissected
and imaged using a fluorescence imaging system (Spectrum CT, PerkinElmer).
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