A single-reflection-attenuated total reflectance with a diamond internal reflection crystal installed in an iS10 FT-IR spectrometer (Thermo Fisher Scientific) was used to obtain spectra from different samples of nanoparticles. Lyophilized powders were placed on the surface of the attenuated total reflectance crystal after background spectra had been collected. Spectra were collected after 64 scans at 4 cm−1 resolution. Data were analyzed using OMNIC software.
Is10 ft ir spectrometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The IS10 FT-IR spectrometer is a laboratory instrument designed for infrared spectroscopy. It uses Fourier transform infrared (FT-IR) technology to measure the absorption of infrared radiation by a sample. The instrument can analyze the composition and structure of various materials by identifying their unique infrared absorption patterns.
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Lab products found in correlation
Smartlab xrd, by Rigaku (2 mentions)
1290 uplc 6540 q tof spectrometer, by Agilent Technologies (1 mentions)
Mci gel, by Mitsubishi Chemical Corporation (1 mentions)
Uv 2401 pc spectrophotometer, by Shimadzu (1 mentions)
Silica gel, by Qingdao Haiyang Chemical (1 mentions)
Gcms model japan, by Thermo Fisher Scientific (1 mentions)
Jsm 7500f scanning electron microscope, by JEOL (1 mentions)
Su5000 field emission sem, by Hitachi (1 mentions)
Sdt q600, by TA Instruments (1 mentions)
Escalab xi, by Thermo Fisher Scientific (1 mentions)
Diamond crystal, by Thermo Fisher Scientific (1 mentions)
Smart itr, by Thermo Fisher Scientific (1 mentions)
Em cpd300 automated critical point dryer, by Leica camera (1 mentions)
16 protocols using is10 ft ir spectrometer
1
Nanoparticle Characterization by IR
2
FT-IR Characterization of Nanoparticles
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Samples were analyzed for FT-IR using an iS10 FT-IR spectrometer (Thermo Fisher Scientific) installed with the attenuated total reflectance (ATR) crystal of a single-reflection-ATR with a diamond internal reflection crystal. Multiple spectra were obtained from different samples of nanoparticles. Background spectra were first collected before samples were mounted. Using OMNIC software, spectra for each sample were collected at 4 cm−1 resolution
3
Spectroscopic Characterization of Compounds
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A Shimadzu UV-2401PC spectrophotometer was used to obtain the UV spectra. A Thermo NICOLET Is10 FT-IR spectrometer was used for IR spectra with KBr pellets. 1D and 2D NMR spectra were recorded on an Avance III-600 spectrometer with TMS as internal standard, and chemical shifts (δ) are expressed in ppm. MS and HR-MS were performed on an Agilent 1290 UPLC/6540 Q-TOF spectrometer. Column chromatography was carried out on Sephadex LH-20 gel (25–100 μm, Pharmacia Fine Chemical Co. Ltd., Stockholm, Sweden), MCI-gel (75–150 µm, Mitsubishi Chemical Corporation, Tokyo, Japan), ODS silica gel (50 µm, YMC Ltd., Kyoto, Japan) and silica gel (200–300 mesh, Qingdao Haiyang Chemical Co. Ltd., Qingdao, China). Thin layer chromatography (TLC) was carried out on silica gel GF254 precoated plates (Qingdao Haiyang Chemical Co. Ltd., Qingdao, China), and spots were detected by spraying with 5% H2SO4 in EtOH followed by heating.
4
Characterization of Hybrid Nanoparticles
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Spectra from non-functionalized hybrid nanoparticles and anti-NTSR1-mAb-functionalized hybrid nanoparticles were collected using a single-reflection attenuated total reflectance (ATR) with a diamond internal reflection crystal installed in a iS10 FTIR spectrometer (Thermo Fisher Scientific, Madison, WI, USA). Lyophilized powders were placed on the surface of the ATR crystal after background spectra had been collected. Spectra were collected after 64 scans at 4 cm−1 resolution. Data were analyzed using OMNIC software.
5
Spectroscopic Characterization of Synthesized Molecules
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The fusion point of the synthesized molecules FTIR was specified via SMP50 Digital Melting Point APP at 120/230 V “Bibby Scientific, Staffordshire, UK”. The IR spectrum was recorded at potassium bromide tablets (4000–400 cm−1) using a Nicolet “iS10FT-IR Spectrometer and Thermos-Fisher Scientific Resolution16”. 1HNMR and 13CNMR) spectra were taped at 300 MHz on a Varian “VX-300 NMR spectrometer” and Bruker EX-400 MHz (13C: 100 MHz) spectrometer) (Bruker, USA) respectively, in D2O (dimethyl sulfoxide was used as an external standard, [δ(1H) = 2.50 and 3.31 ppm, δ (13C) = 39.52 ppm]. The chemical shifts are provided in ppm relating to tetramethyl silane (TMS) at 0.00 ppm. At the Regional Centre for Mycology and Biotechnology, Al-Azhar University, Egypt, mass spectra were collected using a “Thermo Scientific Gcms) Model—Japan”. In addition, the results of the elemental analysis were established and were correct to 0.4%.
6
Chromium-Containing Leather Waste Valorization
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The chromium-containing leather scraps used in this study were from a leather factory in Hebei, China. Tricyanogen chloride (TC), called 2,4,6-trichlorine-1,3,5-triazine, with a molecular weight of 184.14, was obtained from the Wengjiang Chemical Reagent Co. Ltd. in Guangdong, China. TC is soluble in acetone but has slight solubility in water and three chlorine atoms on it can be replaced in different temperature scopes (0–5 °C, 40–50 °C, 80–90 °C respectively) by sulfhydryl, hydroxyl, and amido groups. Gelatin (AR) is from the Chengdu Kelong Co. Ltd., China.
The complete set of equipment for preparing powdered collagen degradants was from the Liaonin Dacheng Biotechnology Co., Ltd., China. The RE-52CS rotary separator was from the Shanghai Xiande Experimental Instrument Co., Ltd., China. The NDJ-8SN rotary viscometer was from the Shanghai Precision and Scientific Instrument Co., Ltd., China. The gel permeation chromatography (GPC) system was from Dongcao Co., Ltd. The EA 3000 elemental analyzer, was from Leeman Labs Inc. The JSM-7500F scanning electron microscope was from the JEOL Co., Ltd. The IS10 FT-IR spectrometer was from Thermo Fisher Scientific, US. The 200PC DSC analyzer and 209F1 TG analyzer were from the NETZSCH Co., Ltd. The Zeta PAL-type laser particle size analyzer was from Malvern Instruments, UK.
The complete set of equipment for preparing powdered collagen degradants was from the Liaonin Dacheng Biotechnology Co., Ltd., China. The RE-52CS rotary separator was from the Shanghai Xiande Experimental Instrument Co., Ltd., China. The NDJ-8SN rotary viscometer was from the Shanghai Precision and Scientific Instrument Co., Ltd., China. The gel permeation chromatography (GPC) system was from Dongcao Co., Ltd. The EA 3000 elemental analyzer, was from Leeman Labs Inc. The JSM-7500F scanning electron microscope was from the JEOL Co., Ltd. The IS10 FT-IR spectrometer was from Thermo Fisher Scientific, US. The 200PC DSC analyzer and 209F1 TG analyzer were from the NETZSCH Co., Ltd. The Zeta PAL-type laser particle size analyzer was from Malvern Instruments, UK.
7
Characterization of PVP-Graphene Oxide Composite
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Atomic force microscope (AFM) images of PVP–GO were obtained using a Multimode 8 in the tapping mode. The PVP–GO sample was dispersed in water and spin coating onto freshly cleaved mica substrates before the test. FTIR spectra were recorded in KBr pellets using an IS10 FTIR spectrometer (Thermo Fisher Scientific Co., USA). Surface morphologies of the samples were observed by a SU5000 field emission SEM (Hitachi Ltd, Japan). Powder XRD spectra were recorded using a PANalytical X′ Pert3 (link) Powder diffractometer with Cu Kα radiation at 40 kV and 40 mA, and a scanning rate of 5° (2θ)/min from 10° to 80°. TGA was carried out using TA Instruments (SDT Q 600) at a heating rate of 10 °C min−1 from 25 °C to 600 °C in nitrogen atmosphere. XPS spectra were obtained with an Axis Ultra DLD (Kratos Ltd, U.K.) paired with a monochromatic Al Kα X-ray source (1486.6 eV).
8
Infrared Spectroscopic Analysis of Samples
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Data infrared spectra were recorded on a Shimadzu FT-IR Affinity-1 Spectrometer and a Nicolet iS10FT IR Spectrometer, Thermo Fisher Scientific, ESCALABXi+, USA. The spectra were obtained between 400 and 4000 cm−1 with a resolution of 4 cm−1 at 25 °C. The sample and potassium bromide (1:100) were mixed, ground into a disc, and scanned in transmittance mode with 16 scans37 (link).
9
FT-IR Characterization of PEO Electrolytes
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FT-IR characterization
was performed on the pure PEOs and the electrolytes using the Thermo
Scientific iS10 FT-IR spectrometer in the wavenumber range of 4000–600
cm–1 with 64 scans for each spectrum.
was performed on the pure PEOs and the electrolytes using the Thermo
Scientific iS10 FT-IR spectrometer in the wavenumber range of 4000–600
cm–1 with 64 scans for each spectrum.
10
FT-IR Analysis of Wood Surface
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Fourier transform-infrared spectroscopy (FT-IR) spectra of the wood surface were recorded on the Nicolet iS10 FT-IR spectrometer, equipped with Smart iTR using an attenuated total reflectance (ATR) sampling accessory attached to a diamond crystal (Thermo Fisher Scientific). The spectra were from 4000 to 650 cm -1 at a spectral resolution of 4 cm -1 , and 64 scans were used. Measurements were performed on four replicates per sample.
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