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Ab6000

Manufactured by Abcam

The Ab6000 is a high-performance laboratory instrument designed for versatile applications. It features precise temperature control, automated pipetting, and data analysis capabilities. The core function of the Ab6000 is to facilitate efficient and accurate sample processing and measurement in a wide range of experimental workflows.

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2 protocols using ab6000

1

Antibody Panel for Stem Cell Research

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Rabbit polyclonal antibodies for NR0B1 (ab60144), NR5A1 (ab65815), and acetyl-histone H3 (ab47915); rabbit monoclonal antibodies for NR5A2 (ab125034), NANOG (ab109250), OCT3/4 (ab109183), AR (ab133273), SOX2 (ab92494), and STAT3 (ab68153); and mouse monoclonal antibodies for H3K4me3 (ab6000), H3K9me2 (ab1220), H3K27me3 (ab6002), α-TUBULIN (ab7750) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, ab9482) were obtained from Abcam (Cambridge, MA). The rabbit polyclonal antibody for acetyl-histone H4 (06-598) was purchased from Millipore (Temecula, CA). The mouse monoclonal anti-FLAG antibody (F1804) was purchased from Sigma–Aldrich (St. Louis, MO).
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2

Antibody Selection for Histone Modification Analysis

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As shown in Table S1, rabbit polyclonal antibodies for acetyl‐histone H3 (ab47915), mouse monoclonal antibodies for H3K9me2 (ab1220), H3K27me3 (ab6002), and H3K4me3 (ab6000) were obtained from Abcam. The rabbit polyclonal antibodies for acetyl‐histone H4 (06‐598) and AR (39781) were purchased from Millipore and Active Motif, respectively. Mouse and rabbit IgG were purchased from Cell Signaling Technology.
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