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Origin2020b software

Manufactured by OriginLab
Sourced in United States

Origin2020b is a data analysis and graphing software developed by OriginLab. It provides tools for importing, managing, and visualizing data, as well as performing statistical analysis.

Automatically generated - may contain errors

3 protocols using origin2020b software

1

Statistical Analysis of Experimental Data

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Data are statistically analyzed using Origin 2020b Software (OriginLab, Northampton, MA, USA) or Graph Pad Prism (Graph Pad Software Inc., San Diego, CA, USA). One-way ANOVA with Turkey’s Multiple Comparison Test was performed to compare the results between groups. All data are presented as mean ± standard deviation (SD). Single asterisk [*] denotes p < 0.05; double asterisks [**] denote p < 0.01; triple asterisks [***] denote p < 0.001 and quadruple asterisks [****] denote p < 0.0001.
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2

Comparative Toxicity Analysis of PPNPs

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Statistical
tests were performed by one-way analysis of variance using Origin
2020b software (Origin Lab Corporation Inc., USA). Tukey’s
test was used to compare the toxic effects of the PPNPs and R-PPNPs
against controls. A significant difference was observed when p < 0.05.
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3

Ratiometric Single-Cell Calcium Imaging

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Ratiometric single cell [Ca2+]cyt imaging was performed on an IX-81 microscope (Olympus)-based system as described previously (Zheng et al., 2013 (link); Zhou et al., 2014 (link)). HeLa cells were incubated in DMEM containing 2 μM Fura-2 AM at 37 °C for 30 minutes. Transfected cells were identified by the presence of fused or co-expressed eGFP. Semrock filters (BrightLine single-band multi-exciter filter set, optimized for Fura-2) were used to minimize contamination of Fura-2 fluorescence by bleed-through of eGFP fluorescence. Data were acquired with Metafluor software (Universal Imaging) and analyzed with Origin2020b software (OriginLab) and are expressed as means ± S.E.M..
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