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Antibodies against grp78

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Antibodies against GRP78 are laboratory reagents used to detect and study the expression of the GRP78 protein. GRP78, also known as BiP, is a molecular chaperone involved in the unfolded protein response within the endoplasmic reticulum. These antibodies can be used in various research applications, such as Western blotting, immunohistochemistry, and immunoprecipitation, to investigate the role of GRP78 in cellular processes.

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Lab products found in correlation

Cda antibody, by Santa Cruz Biotechnology (2 mentions) O glcnac, by Cell Signaling Technology (2 mentions) Phosphorylated iκb α, by Santa Cruz Biotechnology (1 mentions) Total iκbα, by Santa Cruz Biotechnology (1 mentions) Crebh, by Santa Cruz Biotechnology (1 mentions) Pparα, by Santa Cruz Biotechnology (1 mentions) Rabbit insulin elisa kit, by Crystal Chem (1 mentions) N acetylcysteine nac, by Yeasen (1 mentions) Tert butyl hydroperoxide tbhp, by Maclin Biochemical Technology (1 mentions) Antibodies against gapdh, by Proteintech (1 mentions) Bovine serum albumin bsa, by Biosharp (1 mentions) α sma, by Cell Signaling Technology (1 mentions) Beyoecl plus reagent, by Beyotime (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Triton x 100, by Solarbio (1 mentions) Xbp1s, by Cell Signaling Technology (1 mentions) Sglt1 antibodies, by Abcam (1 mentions) Ire1α, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions)

5 protocols using antibodies against grp78

1

Hepatic Metabolic Regulation Protocol

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Chemicals were purchased from Sigma–Aldrich (St. Louis, MO, USA) unless indicated otherwise. Antibodies against phosphorylated JNK, total JNK, phosphorylated IκBα, total IκBα, PPARα, and CREBH were purchased from Santa Cruz Biotechnologies, Inc. (Santa Cruz, CA, USA). Antibodies against GRP78, IRE1a, Xbp1s, β-actin and the secondary antibodies were purchased from Cell Signaling Technologies (Danvers, MA, USA). Antibodies against FGF21 were purchased from Abcam (Boston, MA, USA). The kit for determining ALT, AST, and FFA were purchased from Abcam. The kit for determining TG, TC, and HDL weaspurchased from Fujifilm Wako Diagnostics USA (Mountain View, CA, USA). The glycogen enzyme-linked immunosorbent assay (ELISA) kit was purchased from BioAssay Systems (Hayward, CA, USA). The rabbit insulin ELISA kit was purchased from Crystal Chem (Elk Grove Village, IL, USA). The periodic acids staining kit, Gomori's trichrome staining kit were purchased from Fisher Scientific (Hampton, NH, USA). The assay kits and antibodies information are listed in Table S2.
Wu Q., Liang X., Hou X., Song Z., Bouhamdan M., Qiu Y., Koike Y., Rajagopalan C., Wei H.G., Jiang H., Hish G., Zhang J., Chen Y.E., Jin J.P., Xu J., Zhang K, & Sun F. (2022). Cystic fibrosis rabbits develop spontaneous hepatobiliary lesions and CF-associated liver disease (CFLD)-like phenotypes. PNAS Nexus, 2(1), pgac306.
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2

Protein Isolation and Western Blotting

Cited 2 times
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For protein isolation and western blotting, cells were washed with 1X PBS and lysed in radio-immunoprecipitation assay (RIPA) lysis buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium Deoxycholate, 0.1% SDS supplemented with protease inhibitor tablets). Protein quantification and western blotting were carried out as previously described (36 (link)). Antibody against MUC1 has been previously described (51 (link)). Antibodies against GRP78, CHOP, NRF2 and O-GlcNAc were from Cell Signaling Technology. CDA antibody was from Santa Cruz Technology (Dallas, TX USA).
Olou A.A., King R.J., Yu F, & Singh P.K. (2020). MUC1 Oncoprotein Mitigates ER Stress via CDA-mediated Reprogramming of Pyrimidine Metabolism. Oncogene, 39(16), 3381-3395.
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3

Comprehensive Antibody Sourcing for Cellular Analyses

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Antibodies against GRP78, CHOP, and α-SMA were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibody against KIM-1 (kidney injury molecule-1) was purchased from R&D Systems (Minneapolis, MN, USA). Antibodies against GAPDH were purchased from Proteintech (Chicago, IL, USA). Antibodies against ATF-4 were purchased from Bioss (Beijing, China). Antibodies against β-tubulin were purchased from Elabscience (Wuhan, China). Antibody against Fibronectin 1 (FN1) were purchased from ZEN-BIOSCIENCE (Chengdu, China). Secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Invitrogen (Carlsbad, CA, USA). N-acetylcysteine (NAC) was purchased from Yeasen (Shanghai, China). tert-Butyl hydroperoxide (TBHP) was purchased from Macklin (Shanghai, China). Fetal bovine serum (FBS) was purchased from Suero fetal bovine ester, Natocor-Industria Biologica (Cordoba, Argentina). Bovine serum albumin (BSA) was purchased from Biosharp (Anhui, China). Triton X-100 was purchased from SolarBio (Beijing, China). Radioimmunoprecipitation assay (RIPA) buffer was purchased from Epizyme (Shanghai, China). BeyoECL Plus reagent was purchased from Beyotime Biotechnology (Shanghai, China).
Yin S., Liu J., Zhao X., Dong H., Cao Y., Zhang S., Dong X., Zhang G., Jin S., Shi W., Xiang F., Gong J., Wang Z., Han B., Zhang N., Tan X, & Wang Z. (2023). Chitosan oligosaccharide attenuates acute kidney injury and renal interstitial fibrosis induced by ischemia-reperfusion. Renal Failure, 45(1), 2238831.
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4

Validation of SGLT1 Antibody Specificity

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Chemicals were purchased from Sigma-Aldrich unless indicated otherwise. CFTR antibodies were obtained from the CF foundation. Antibodies against GRP78, IRE1α, XBP1s, and β-actin were purchased from Cell Signaling Technology. SGLT1 antibodies were purchased from Abcam and Invitrogen. The specificity of the SGLT1 antibody for IHC staining was confirmed by using an IgG isotype control, a secondary antibody–only control, and by using a nontargeting primary antibody control (Supplemental Figure 4). The secondary antibodies were from LI-COR Biosciences and Jackson ImmunoResearch Laboratories. The PAS staining kit was purchased from Thermo Fisher Scientific. The assay kits and antibody information are listed in Supplemental Table 4.
Liang X., Hou X., Bouhamdan M., Sun Y., Song Z., Rajagopalan C., Jiang H., Wei H.G., Song J., Yang D., Guo Y., Zhang Y., Mou H., Zhang J., Chen Y.E., Sun F., Jin J.P., Zhang K, & Xu J. (2024). Sotagliflozin attenuates liver-associated disorders in cystic fibrosis rabbits. JCI Insight, 9(6), e165826.
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5

Protein Isolation and Western Blotting

Cited 2 times
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For protein isolation and western blotting, cells were washed with 1X PBS and lysed in radio-immunoprecipitation assay (RIPA) lysis buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium Deoxycholate, 0.1% SDS supplemented with protease inhibitor tablets). Protein quantification and western blotting were carried out as previously described (36 (link)). Antibody against MUC1 has been previously described (51 (link)). Antibodies against GRP78, CHOP, NRF2 and O-GlcNAc were from Cell Signaling Technology. CDA antibody was from Santa Cruz Technology (Dallas, TX USA).
Olou A.A., King R.J., Yu F, & Singh P.K. (2020). MUC1 Oncoprotein Mitigates ER Stress via CDA-mediated Reprogramming of Pyrimidine Metabolism. Oncogene, 39(16), 3381-3395.
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