PPAR-γ (ab45036), interleukin (IL)-1β (ab9722), tumor necrosis factor-alpha (TNF-α; ab66579), and F4/80 (ab6640) antibodies were obtained from Abcam (Cambridge, UK). CD45 antibody (sc-52491) and rosiglitazone (sc-202795) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). IL-6 (12912S), NF-κB p65 (8242), phospho-NF-κB p65 (3033), stress-activated protein kinase (SAPK)/Jun-amino-terminal kinase (JNK; 9258), phospho-SAPK/JNK (9255S), p38 MAPK (9212L), phospho-p38 MAPK (4631L), p44/42 MAPK (Erk1/2) (4695S), and phospho-p44/42 MAPK (Erk1/2) (9101S) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Invitrogen Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (A-11058); Invitrogen Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (A-11055); and Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A-21206) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Horseradish peroxidase (HRP)-conjugated anti-β-actin antibody (a5316) was obtained from Sigma-Aldrich (St. Louis, MO, USA), and 4′,6-diamidino-2-phenylindole (DAPI; H-1200) and mounting medium (H-5000) were obtained from Vector Laboratories (Burlingame, CA, USA).
F4 80 ab6640
Manufactured by Abcam
Sourced in United Kingdom, United States
F4/80 (ab6640) is a monoclonal antibody that binds to the F4/80 antigen, a cell surface glycoprotein expressed by mature mouse macrophages. This antibody can be used in various applications such as flow cytometry, immunohistochemistry, and western blotting to detect and quantify macrophage populations.
Automatically generated - may contain errors
Lab products found in correlation
Phospho p44 42 mapk erk1 2 9101s, by Cell Signaling Technology (1 mentions)
4 6 diamidino 2 phenylindole dapi h 1200, by Vector Laboratories (1 mentions)
Tnf α ab66579, by Abcam (1 mentions)
Fitc donkey anti rabbit igg, by BioLegend (1 mentions)
Anti akt pan antibody, by Cell Signaling Technology (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Hoechst 33258 dye, by Merck Group (1 mentions)
Pf 573228, by Selleck Chemicals (1 mentions)
Fitc donkey anti mouse igg, by BioLegend (1 mentions)
Ly294002, by Selleck Chemicals (1 mentions)
P65 cst 8242s, by Cell Signaling Technology (1 mentions)
Perilipin 1, by Cell Signaling Technology (1 mentions)
Ly6g 6c, by BioLegend (1 mentions)
3 protocols using f4 80 ab6640
1
Molecular Signaling Pathway Analysis
2
Peptide Characterization and Cell Culture Protocols
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The peptides were synthesized, modified for stability by acetylation at the NH2 termini and amidation at the COOH termini, and characterized by mass spectrometry (>90% purity) at GenScript (Piscataway, NJ, USA). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), antibiotic–antimicotic solutions, and trypsin were purchased from Invitrogen (Carlsbad, CA, USA). Fibronectin (F0895), 5-bromo-2’-deoxyuridine (BrdU), TRITC-phalloidin, Hoechst 33258 dye and all chemicals were from Sigma-Aldrich (St. Louis, MO, USA). Antibodies for BrdU (GTX42641) and fibronectin (GTX34727) were from GeneTex (Taipei, Taiwan). Phospho-FAK (Tyr397) antibody (#3283), anti-FAK antibody (#13009), phospho-Akt (Thr308) antibody (#13038), and anti-Akt (pan) antibody (#4691) were purchased from Cell Signaling Technology (Danvers, MA). Antibodies for integrin alpha 5 antibody (ab25251), integrin beta 1 (ab179471), Ly6g (PE/Cy7, ab25514), and F4/80 (ab6640) were from Abcam (Cambridge, MA, USA). FITC-donkey anti-rabbit IgG and FITC-donkey anti-mouse IgG were purchased from BioLegend (San Diego, CA, USA). NVP-TAE226, PF-573228, and LY-294002 were purchased from Selleckchem (Houston, TX, USA).
3
Immunofluorescence Staining for Cell Markers
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Immunofluorescence staining was conducted according to standard procedures. Paraffin sections of tissue or cell climbing sheets were incubated with specific primary antibodies (GCA, PA5-77127, Invitrogen,1:200; F4/80, ab6640, abcam,1:200; PHB2, sc-133094, Santa Cruz,1:200; P65, CST8242S, Cell Signaling Technology,1:400, Ly6g/6c,Biolegend,108403,1:200;Perilipin-1,Cell Signaling Technology,9349 S,1:200) at 4 °C overnight. After washing with PBS for 3 times, the sections or sheets were incubated with corresponding fluorescent secondary antibodies. The cell nuclei were labeled with DAPI. The cytoplasmic membrane was stained with Dil. The results were imaged by fluorescence microscope or confocal microscopy.
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