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Goat anti mouse igg hrp abs20001

Manufactured by Absin
Sourced in China

Goat anti-mouse IgG-HRP (abs20001) is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed for the detection of mouse immunoglobulin G (IgG) in various immunoassays and immunochemical applications.

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4 protocols using goat anti mouse igg hrp abs20001

1

Cell Culture and Antibody Reagents

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AGS, HGC27 and GES-1 cell lines were purchased from the Cell Bank of the Chinese Academy of Sciences and cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS). FBS and RPMI-1640 were purchased from Gibco (NY, USA). The cells were placed in an incubator at 37°C and a CO2 concentration of 5%. Antibodies against TP53 (#2527), BRCA1 (#14823), PIK3CA (#4255), AKT1 (#2938), and SMARCA4 (#49360) were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against HRAS (abs137096) and beta actin antibody (abs132001) were obtained from Absin (Shanghai, China). The secondary antibodies used in this study include goat anti-mouse IgG-HRP (abs20001) and goat anti-rabbit IgG-HRP (abs20002), both of which can be obtained from Absin.
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2

Autophagy Regulation in Cell Migration

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FBS and RPMI-1640 were purchased from Gibco (NY, USA). 3-MA and rapamycin were purchased from MCE (Shanghai, China). Autophagy double-labeled adenovirus (mRFP-GFP-LC3) was purchased from Hanbio Biotechnology Co., Ltd., and antibodies against E-cadherin (#14472), N-cadherin (#13116), Vimentin (#5741), LC3-I/II (#3868), β-actin (#4970) and Beclin1 (#3495S) were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against FOXK1 (ab18196) and MMP-9 (ab38898) were purchased from Abcam (Cambridge, MA, USA), and antibodies against MAZ (NB100-86984) were purchased from Novus (Columbus, MO, USA). The secondary antibodies used in this study included goat anti-mouse IgG-HRP (abs20001) and goat anti-rabbit IgG-HRP (abs20002), both of which are available from Absin (Shanghai, China).
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3

TRAIL and 5-FU Combination Therapy

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The GES-1, AGS and HGC27 cell lines were purchased from the cell bank of the Chinese Academy of Sciences and cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS). The incubator conditions were 37°C and 5% CO 2 (12) . FBS and RPMI-1640 medium were purchased from Gibco (New York, USA). Recombinant human TRAIL (#T9701) and 5-FU (#F6627) were purchased from Sigma-Aldrich (Munich, Germany). The small-molecule inhibitors U0126 (#M1977), SP600125 (#M2076) and SB202190 (#M2062) were obtained from AbMole (Houston, USA). Primary antibodies against the following proteins were purchased from Cell Signaling Technology (Beverly, MA, USA): TRAIL (#3219), Caspase-3 (#9662), Caspase-8 (#9746), Caspase-9 (#9502), Cleaved Caspase-3 (#9661), Cleaved Caspase-8 (#9496), Cleaved Caspase-9 (#9505), PARP (#9532), Bid (#2002), DR4 (#42533), DR5 (#8047), c-IAP1 (#7065), c-IAP2 (#3130), Bcl-2 (#4223), Mcl-1 (#94296), Erk (#4695), JNK (#9252), p38 (#8690), phospho-Erk (#4370), phospho-JNK (#4668), phospho-p38 (#4511) and β-actin (#4970). The secondary antibodies used in this study included goat anti-mouse IgG-HRP (abs20001) and goat anti-rabbit IgG-HRP (abs20002), both of which were obtained from Absin (Shanghai, China).
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4

TRAIL and 5-FU Combination Therapy

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The GES-1, AGS and HGC27 cell lines were purchased from the cell bank of the Chinese Academy of Sciences and cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS). The incubator conditions were 37°C and 5% CO 2 (12) . FBS and RPMI-1640 medium were purchased from Gibco (New York, USA). Recombinant human TRAIL (#T9701) and 5-FU (#F6627) were purchased from Sigma-Aldrich (Munich, Germany). The small-molecule inhibitors U0126 (#M1977), SP600125 (#M2076) and SB202190 (#M2062) were obtained from AbMole (Houston, USA). Primary antibodies against the following proteins were purchased from Cell Signaling Technology (Beverly, MA, USA): TRAIL (#3219), Caspase-3 (#9662), Caspase-8 (#9746), Caspase-9 (#9502), Cleaved Caspase-3 (#9661), Cleaved Caspase-8 (#9496), Cleaved Caspase-9 (#9505), PARP (#9532), Bid (#2002), DR4 (#42533), DR5 (#8047), c-IAP1 (#7065), c-IAP2 (#3130), Bcl-2 (#4223), Mcl-1 (#94296), Erk (#4695), JNK (#9252), p38 (#8690), phospho-Erk (#4370), phospho-JNK (#4668), phospho-p38 (#4511) and β-actin (#4970). The secondary antibodies used in this study included goat anti-mouse IgG-HRP (abs20001) and goat anti-rabbit IgG-HRP (abs20002), both of which were obtained from Absin (Shanghai, China).
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