Anti cd54 pe

Manufactured by Beckman Coulter

The Anti-CD54-PE is a fluorochrome-conjugated monoclonal antibody that specifically binds to the CD54 (ICAM-1) antigen on the surface of cells. CD54 is a cell adhesion molecule involved in various cellular interactions and processes. The Anti-CD54-PE can be used in flow cytometry applications to identify and characterize cells expressing the CD54 antigen.

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Lab products found in correlation

Anti hla abc apc, by Thermo Fisher Scientific (2 mentions) Anti pd l1 pe, by BioLegend (2 mentions) Aria 2 cell sorter, by BD (1 mentions) Anti hla dr pc7, by Beckman Coulter (1 mentions) Kaluza, by Beckman Coulter (1 mentions) Facsdiva software, by BD (1 mentions) Gallios flow cytometer, by Beckman Coulter (1 mentions) Anti mitf, by Santa Cruz Biotechnology (1 mentions) Anti hmb 45, by Agilent Technologies (1 mentions) Anti hla dr pecy7, by Beckman Coulter (1 mentions) Anti pd l2, by BioLegend (1 mentions) Mouse anti tyrosinase, by Santa Cruz Biotechnology (1 mentions) Anti cd3, by BD (1 mentions)

2 protocols using anti cd54 pe

Isolating Ma-Mel-36 Subpopulations by Flow Cytometry

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The following directly labelled antibodies were used for staining of cellular surface markers: anti-HLA-ABC-APC (eBiosciences, clone W6/32; 1 μl), anti-CD54-PE (Beckmann Coulter, clone 84H10; 2.5 μl), anti-PD-L1-PE (Biolegend, clone 29E2A3; 5 μl) and anti-HLA-DR-PC7 (Beckmann Coulter, clone Immun-357; 2.5 μl). After fixation, stained cells were analysed by flow cytometry on a Gallios flow cytometer (Beckmann Coulter) and Kaluza (Beckman Coulter) software, respectively, for data analysis. In order to isolate specific Ma-Mel-36 subpopulations, cells were stained with anti-HLA-DR-PC7 and sorted based on the specific expression of the surface markers by flow cytometry on an Aria II cell sorter and the FACS Diva software (BD Biosciences).

Sucker A., Zhao F., Pieper N., Heeke C., Maltaner R., Stadtler N., Real B., Bielefeld N., Howe S., Weide B., Gutzmer R., Utikal J., Loquai C., Gogas H., Klein-Hitpass L., Zeschnigk M., Westendorf A.M., Trilling M., Horn S., Schilling B., Schadendorf D., Griewank K.G, & Paschen A. (2017). Acquired IFNγ resistance impairs anti-tumor immunity and gives rise to T-cell-resistant melanoma lesions. Nature Communications, 8, 15440.

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Murine Monoclonal Antibodies for Immunodetection

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The following murine mAbs were used for IHC: W6/32 to detect HLA class I antigens (Dianova); bbm.1 to stain for β2m (kindly provided by G. Moldenhauer, German Cancer Research Center, Heidelberg, Germany); anti–HMB-45 (Dako) to detect melanoma cells; anti-CD3 (BD Pharmingen) to stain for T cells.
For flow cytometry, the mouse mAbs were as follows: anti–HLA-ABC-APC (eBiosciences), anti–CD54-PE and anti–HLA-DR-PECy7 (Beckmann Coulter), anti–PD-L1-PE and anti–PD-L2 (Biolegend), anti–B7-H3 (R&D Systems), anti–B7-H4 (eBiosciences); L243 was used for detection of HLA-DR molecules (17 (link)) and HC10 for labelling of β₂m-free HLA heavy chains (18 (link), 19 (link)).
The following antibodies were used for Western blot analysis: mouse anti–Melan-A/MART-1 (Zytomed), mouse anti-Tyrosinase and anti-MITF (Santa Cruz Biotechnology), rabbit anti-DCT/TRP2 (kindly provided by V. Hearing, National Cancer Institute, NIH, Bethesda); mouse anti-STAT1, rabbit anti-phospho(p)STAT1, rabbit anti-JAK1, rabbit anti-GAPDH (Cell Signaling Technology); rabbit anti-IRF1 and mouse anti-β2m (Santa Cruz Biotechnology); rabbit anti-β2m (Sigma); mouse anti-TAP1 (NOB-1) and mouse anti-tapasin (TO-3; ref. 20 (link)); mouse mAb HC10 (18 (link), 19 (link)).

Sucker A., Zhao F., Real B., Heeke C., Bielefeld N., Maβen S., Horn S., Moll I., Maltaner R., Horn P.A., Schilling B., Sabbatino F., Lennerz V., Kloor M., Ferrone S., Schadendorf D., Falk C.S., Griewank K, & Paschen A. (2014). Genetic Evolution of T-cell Resistance in the Course of Melanoma Progression. Clinical cancer research : an official journal of the American Association for Cancer Research, 20(24), 6593-6604.

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