V 650q1 spectrometer

PGA (MW = 1.5–2.5 × 10⁶) was purchased from Wako Pure Chemicals, Tokyo, Japan. Thermostable phosphorylase from Aquifex aeolicus VF5 was supplied from Ezaki Glico Co. Ltd., Osaka, Japan.^{19,43,44 (link)} The primer-grafted PGA was synthesized according to the literature procedure.^{38 (link)1}H NMR (D₂O) δ 1.86–2.01, 2.01–2.18 (br, β-CH₂ of PGA), 2.30–2.42 (br, γ-CH₂ of PGA), 3.40–4.08 (br, sugar protons of H2–H6), 4.12–4.25 (br, α-CH of PGA), 5.18, 5.41 (br s, H1 of primer). The DS for the grafting was determined by the integrated ratio of the H1 signal of the primer to the γ-CH₂ signal of PGA to be 70.2%. The guest polymer, PLLA, was synthesized by ring-opening polymerization of l-lactide initiated with l-lactic acid.^{45 (link)} The M_n value was calculated by the integrated ratio of the main-chain signal to the terminal signal to be 1540. Other reagents and solvents were available commercially and used without further purification. ¹H NMR spectra were recorded on JEOL ECX400 spectrometer. XRD measurements were performed using a PANalytical X'Pert Pro MPD diffractometer with Ni-filtered Cu Kα radiation (λ = 0.15418 nm). UV-vis measurements were conducted using a Jasco V-650Q1 spectrometer. The DLS measurements were obtained on a Zetasizer Nano ZS (Malvern Instruments). IR spectra were recorded on a PerkinElmer Spectrum Two spectrometer.