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2 protocols using folin phenol reagent

1

Antioxidant Capacity Evaluation of Gallic Acid

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Gallic acid (CAS:149-91-7) was purchased from Shanghai Yuanye Bio-Technology Co., Ltd. China. Folin phenol reagent (Catalog Number: 73104861), Sodium Carbonate (CAS:497-19-8), Potassium persulfate (CAS:7727-21-1), MEthanol (CAS: 67-56-1) and Ethanol (CAS:64-17-5) were purchased from Sinopharm Chemical Reagent Co., Ltd. (SCR), China. DPPH (CAS:1898-66-4) and Trolox (CAS:53188-07-1) were purchased from Sigma–Aldrich (USA). ABTS (CAS:30931-67-0) was purchased from TCI Europe N.V. (Belgium). All other chemical substances and solvents used were of analytical grade and stored in ideal conditions according to the manufacturer’s specifications.
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2

Antioxidant and Antiglycation Assays

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BSA and gallic acid were provided by the China National Pharmaceutical Group. Glu, FeSO4, 0.01% H2O2, K2S2O8, Folin phenol reagent, potassium ferricyanide, ethylenediaminetetraacetic acid disodium salt (EDTA), and trichloroacetic acid (TCA) were obtained from Sinopharm Chemical Reagent Co. (Shanghai, China). Lactic acid bacteria (LAB) preserved in a laboratory, arginine (Arg), glyoxal (GO) (40% aqueous solution), Methylglyoxal (40% aqueous solution), aminoguanidine, diammonium salt (ABTS), 1-anilino-8-naphthalene sulfonate (ANS), 1,1-diphenyl-2-picrylhy-drazyl (DPPH), and ortho-phthalaldehyde (OPA) were purchased from Macklin Biochemical Co. (Shanghai, China). In addition, ο-phenylenediamine (ο-PDA), nitroblue tetrazolium (NBT), 2,4-dinitrophenylhydrazine (2,4-DNPH), 1,10-phenanthroline, and 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB) were purchased from Aladdin (Shanghai, China). All reagents were of analytical grade.
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