Mouse specific primers

Manufactured by Thermo Fisher Scientific

Mouse-specific primers are short DNA sequences designed to target and amplify specific regions within the mouse genome. These primers are used in various molecular biology techniques, such as polymerase chain reaction (PCR), to detect and quantify the presence of target genes or sequences in mouse samples.

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Lab products found in correlation

Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (1 mentions) Silencer select sirna, by Thermo Fisher Scientific (1 mentions) Dna engine opticon system, by Bio-Rad (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Maxima sybr green master mix, by Thermo Fisher Scientific (1 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Specific primers (81 citations) Mouse primers (2 citations) Mouse specific Taqman gene primers (2 citations) TaqMan assay mouse-specific primers (2 citations)

2 protocols using mouse specific primers

siRNA-mediated knockdown of Hsp90b1 and 14-3-3ζ

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siRNA duplexes of siHsp90b1 (Gene ID: 22027) and si14-3-3ζ (Gene ID: 22631) oligos were purchased from Silencer™ Select siRNAs (Thermo Fisher Scientific). The siRNA oligos were transfected into cells by using Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific) according to manufacturer’s instructions. The Silencer™ Select Negative Control was used as negative control. The sequences of mouse-specific primers (Thermo Fisher Scientific) to detect the knockdown effect of tt-DDE target genes were: Hsp90b1: (forward) 5’-TCGTCAGAGCTGATGATGAAGT-3’ and (reverse) 5’- GCGTTTAACCCATCCAACTGAAT-3’, 14-3-3ζ: (forward) 5’-GAAAAGTTCTTGATCCCCAATGC-3’ and (reverse) 5’-TGTGACTGGTCCACAATTCCTT-3’.

Wang Y., Dattmore D.A., Wang W., Pohnert G., Wolfram S., Zhang J., Yang R., Decker E.A., Sing Stephen Lee K, & Zhang G. (2019). trans, trans-2,4-decadienal, a lipid peroxidation product, induces inflammatory responses via Hsp90- or 14-3-3ζ-dependent mechanisms. The Journal of nutritional biochemistry, 76, 108286.

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Colon Tissue RNA Extraction and qPCR Analysis

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The colon tissues were frozen by liquid nitrogen and ground. Total RNA was isolated from the colon tissues using TRIzol reagent (Ambion, Austin, TX) according to the manufacturer’s instructions. The RNA was reverse transcribed into cDNA using a High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Foster City, CA) according to the manufacturer’s instruction. RT-qPCR was carried out with a DNA Engine Opticon system (Bio-Rad Laboratories, Hercules, CA) with Maxima SYBR-green Master Mix (Thermo Fisher Scientific). The sequences of mouse-specific primers (Thermo Fisher Scientific) were listed in Table S2. Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) was used as an internal control.

Yang H., Sanidad K.Z., Wang W., Xie M., Gu M., Cao X., Xiao H, & Zhang G. (2019). Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved. Gut Microbes, 12(1), 1690364.

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