As 400 400 mhz spectrometer

Unless otherwise noted, all reagents were purchased from Sigma-Aldrich (St. Louis, MO) and used without further purification. BODIPY FL and BODIPY 650/665 succinimidyl ester were purchased from Invitrogen (Carlsbad, CA). Olaparib (AZD2281) was purchased from Selleck Chemicals (Houston, TX). 4-[[4-Fluoro-3-(piperazine-1-carbonyl)phenyl]methyl]-2H-phthalazin-1-one and olaparib-BODIPY FL were synthesized as described elsewhere (40 –42 (link)). HPLC-ESI-MS analyses and HPLC purifications were performed on a Waters (Milford, MA) LC-MS system. For LC-ESI-MS analyses, a Waters XTerra^® C18 5 μm column was used. For preparative runs, an Atlantis^® Prep T3 OBDTM 5 μM column was used. High-resolution ESI mass spectra were obtained on a Bruker Daltonics APEXIV 4.7 Tesla Fourier Transform mass spectrometer (FT-ICR-MS) in the Department of Chemistry Instrumentation Facility at the Massachusetts Institute of Technology. Proton nuclear magnetic resonance (¹H NMR) spectra were recorded on a Varian AS-400 (400 MHz) spectrometer. Chemical shifts for protons are reported in parts per million (ppm) and are referenced against the dimethylsulfoxide lock signal (¹H, 2.50 ppm). Data are reported as follows: chemical shift, multiplicity (s = singlet, d = doublet, t = triplet, m = multiplet), coupling constants (Hz) and integration.