Rat igg1 pe

Manufactured by Thermo Fisher Scientific

Rat IgG1-PE is a lab equipment product that serves as a fluorescent label. It is composed of a rat immunoglobulin G1 (IgG1) antibody conjugated to the R-Phycoerythrin (PE) fluorescent dye.

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PE rat IgG1 κ isotype control (3 citations) Rat IgG1 (6 citations) IgG1-PE (8 citations)

2 protocols using rat igg1 pe

CFSE Proliferation Assay with Cytokine Stimulation

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CFSE staining was done using 5 µM CFSE (Invitrogen, Cat# C34554) as described by Parish et al. [42] (link) and stimulated with cytokines for 6 days prior to flow cytometric analysis. Antibodies used: CD8-APC (BD, Cat# 345775), CD3-APC (BD, Cat# 555335), CD4-APC (Biolegend, Cat# 300514), CD56-APC (eBioscience, Cat#17-0569-42), CD16-PE (Biolegend, Cat#302007), HLA-DR-PE (Biolegend, Cat# 307605), NKG2D-APC (RnD Systems, Cat# FAB139A). For intracellular staining, IL-6-PE (eBioscience, Cat#12-7069) and IFN-γ-FITC (BD, Cat# 554700) were used. Isotype controls were IgG1 APC (BD, cat # 555751), rat IgG1-PE (Invitrogen, Cat # R104) and IgG1-FITC (BD, Cat #555748). PBMCs were incubated for 6 h +/− LPS (Sigma-Aldrich, L2654, 1 µg/mL) and cells were stained using the BD Cytofix/Cytoperm Kit (BD, Cat# 554714), Golgistop (BD, Cat# 554724) or Golgiplug (BD, Cat# 555029) according to the manufacturer’s protocol. Samples were run on an Accuri C6 Flow cytometer and data were analyzed using FCS Express v. 3.

Reichwald K., Jørgensen T.Z., Tougaard P, & Skov S. (2014). TL1A Induces TCR Independent IL-6 and TNF-α Production and Growth of PLZF+ Leukocytes. PLoS ONE, 9(1), e85793.

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Multicolor Flow Cytometry Staining

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The following were purchased from from BD PharMingen: (i) Purified mouse anti-human monoclonal antibodies (mAbs) against HLA-DR, CD80, CD86, CD40, CD83, CD38, CD34, and CD117; (ii) CD14 phycoerythrin (PE)–conjugated mouse anti-human mAbs against CD4; (iii) fluorescein isothiocyanate (FITC)–conjugated anti-CD4 (RPA-T4, IgG1) and anti-CD8 (RPA-T8, IgG1); (iv) FITC-, PE-conjugated matching isotype IgG1, IgG2a, and IgG2b controls; and (v) purified mouse monoclonal IgG1 (MOPC-21) isotype control. The mAb DF3 (anti–MUC1-N) has been described previously (27 (link)). Anti-human CD4 TC-conjugated, matching isotype control (IgG2a), PE-conjugated anti-human mAbs against IFN-γ (mouse IgG1-B27), and PE-conjugated matching isotype controls (rat IgG1-PE and mouse IgG1-PE) were purchased from Invitrogen. FITC-conjugated goat anti-mouse (IgG1) was purchased from Chemicon International.

Rosenblatt J., Stone R.M., Uhl L., Neuberg D., Joyce R., Levine J.D., Arnason J., McMasters M., Luptakova K., Jain S., Zwicker J.I., Hamdan A., Boussiotis V., Steensma D.P., DeAngelo D.J., Galinsky I., Dutt P.S., Logan E., Bryant M.P., Stroopinsky D., Werner L., Palmer K., Coll M., Washington A., Cole L., Kufe D, & Avigan D. (2016). Individualized vaccination of AML patients in remission is associated with induction of antileukemia immunity and prolonged remissions. Science translational medicine, 8(368), 368ra171.

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