1×103 cells were seeded at 96-well plates and cultured for 24 h. The cell proliferation assay was performed on days 1, 2, 3, 4, and 5 using Cell Counting Kit-8 (CCK-8) (Dojindo Laboratories, Japan). 10 μl CCK-8 reagent was added to each well and then the plate was incubated for 2 h at 37°C. At the endpoint of incubation, the absorbance was measured at 450 nm using a Vmax microplate spectrophotometer (Molecular Devices, Sunnyvale, CA). Each sample was assayed in triplicate and repeated 3 times independently.
Vmax microplate spectrophotometer
Manufactured by Molecular Devices
Sourced in United States
The Vmax microplate spectrophotometer is a laboratory instrument designed for absorbance measurements in microplates. It is capable of performing accurate and reliable optical density (OD) measurements across a wide range of applications.
Automatically generated - may contain errors
Lab products found in correlation
Cell counting kit 8 cck 8, by Dojindo Laboratories (2 mentions)
Cell count kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Cck 8, by Molecular Devices (1 mentions)
Cck 8, by Dojindo Laboratories (1 mentions)
Ez cytox, by Molecular Devices (1 mentions)
Ez cytox, by Daeil Lab Service (1 mentions)
Facscalibur flow cytometer system, by BD (1 mentions)
Celltiter 96 aqueous one solution cell proliferation assay, by Promega (1 mentions)
12 protocols using vmax microplate spectrophotometer
1
Cell Proliferation Assay with CCK-8
2
Cell Proliferation Assay with CCK-8
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Cell Count Kit-8 (CCK-8) (Dojindo Molecular Technologies, Tokyo, Japan) was used to evaluate cell proliferation. PC3 and DU145 cells stably transfected with pCDH-miR-4638-5p, pCDH-miR-4638-5p sponge, pCDH-shKidins220, pCDH-shAKT and pCDH-vector controls were seeded into 96-well plates at density of 2 × 103 cells per well and cultured for 1 day, 2 days, 3 days, 4 days, 5 days and 6 days. Each well of cells were then cultured in 20 μl of CCK-8 solution in addition to the 200 μl of culture medium for 1.5 hours at 37°C. Absorbance at 450 nm was measured using a Vmax microplate spectrophotometer (Molecular Devices). LNCaP cells transfected with pCDH-miR-4638-5p sponge and pCDH-vector control were seeded into 96-well plates (also 2 × 103 cells per well) in the RPMI 1640 medium supplemented with 10% charcoal-stripped FBS and cultured for 1 day, 2 days, 3 days, 4 days and 5 days. Then the cell proliferation ability was evaluated using CCK-8 solution as described above.
3
Cell Proliferation Measurement by MTS Assay
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Cell proliferation was measured using the CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS). After culturing for 24, 48, and 72 h, 10 μL of MTS reagent was added to each well and incubated for 4 h at 37°C. Then, the absorbance was measured at 490 nm using a Vmax microplate spectrophotometer (Molecular Devices, Sunnyvale, CA). Each sample was assayed thrice.
4
Cell Proliferation Assay Protocol
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Cell proliferation rates were measured using Cell Counting Kit-8 (CCK-8) (Dojindo Laboratories, Japan). Indicated cells were seeded at 1×103 per well in 96-well plate. The cell proliferation assay was performed on days 1, 2, 3, 4, and 5. 10 ml CCK-8 reagent was added to each well and then the plate was incubated for 2 h at 37°C. At the endpoint of incubation, the absorbance was measured at 450 nm using a Vmax microplate spectrophotometer (Molecular Devices, Sunnyvale, CA). Each sample was assayed in triplicate and repeated 3 times independently.
5
Cell Proliferation Assay with CCK-8
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Cell aliquots (100 μL) were transferred into each well of 96-well microtiter plates at a concentration of 1 × 104 cells/mL. CCK-8 (Dojindo Laboratories, Japan) was used to test the cell proliferation ability every 24 h and would last 6 d. Each day we added 10 μL of CCK-8 reagents into each well and then incubated the plates at 37 °C for 2 h. After the incubation, the absorbance of each well was measured at 450 nm using the Vmax microplate spectrophotometer (Molecular Devices, CA).
6
Cell Viability Assay Using CCK-8
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Approximately 2,000 stably transfected cells were distributed onto 96-well plates. Once attached, they were prepared as per their individual study procedure. Subsequently, 20 µl Cell Counting Kit−8 reagent (CK04, Dojindo Kumamoto, Japan) was supplemented to individual wells and retained for 2 h. Vmax microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA) was used to measure the absorbance at 450 nm wavelength. These assays were performed in triplicate.
7
Cell Proliferation Assay with CCK8
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Cell proliferation was detected using the CCK8 according to the manufacturer’s protocol. The cells were seeded at 1×103 per well in a 96-well plate and treated as experiment design. Then, 10 μL of CCK8 reagent was added to each well and incubated for 4 hours at 37°C. VMax® microplate spectrophotometer (Molecular Devices LLC, Sunnyvale, CA, USA) was used to measure the absorbance at 490 nm.
8
Measuring 3T3-L1 Cell Viability
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Cell viability was measured using the EZ-CyTox (Daeil Lab Service, Seoul, Republic of Korea). 3T3-L1 preadipocytes were seeded into 96-well plates at a density of 3 × 103 cells per well. The cells were treated with sinapic acid (1 μM to 20 μM) or with dimethyl sulfoxide (DMSO), as a control and were incubated for 48 h. 3T3-L1 cells were added with a medium containing EZ-CyTox solution (0.01 ml/well) and incubated at 37°C for 1 h. Absorbance was measured at 450 nm using a Vmax microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA).
9
Cell Proliferation Assay Using CCK-8
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The CCK-8 proliferation assay kit was purchased from Nanjing KeyGen Biotech Co., Ltd. (catalog no. KGA317, Nanjing, China). The cell proliferation assay was performed over 4 days every 24 h with 1×103 cells/well. CCK-8 reagent (10 µl) was added to each well, and then the plate was incubated for 2 h at 37°C. After that, the absorbance was measured at 450 nm using a Vmax microplate spectrophotometer (Molecular Devices, LLC, Sunnyvale, CA, USA) as previously described (28 (link)). All measurements were performed in quintuplicate in three separate experiments.
10
Melanin Content Quantification Assay
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Melanoma cells and melanocytes were treated with milk exosomes (20 and 50 μg/mL) for 48 h. After washing twice with ice-cold PBS, the cells were centrifuged at 2500× g for 10 min. The cell pellets were resuspended at 90 °C for 30 min in 1 N NaOH containing 10% of dimethyl sulfoxide. Total melanin content was measured on the Vmax microplate spectrophotometer (Molecular Devices) at 405 nm.
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