Mouse 4T1 tumor sections were deparaffinized by deparaffinization buffer (Solarbio life science). Improved Citrate Antigen Retrieval Solution (Beyotime Biotechnology) and endogenous peroxidase blocking buffer (Beyotime Biotechnology) were used for antigen retrieval and elimination of endogenous peroxidase activity, respectively. Then, the sections were blocked with goat serum in PBS for 30 min followed by incubating with antibodies at 4 °C overnight. After washing three times with PBS, tissues were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies, and DAB (3,3′-diaminobenzidine) HRP Color Development Kit was used for visualization. The commercially purchased antibodies used for IHC staining are shown in Table 3 .
Deparaffinization buffer
Manufactured by Solarbio
Deparaffinization buffer is a solution used in the process of removing paraffin wax from tissue samples prior to further processing. It helps to dissolve and remove the paraffin wax to prepare the samples for subsequent analysis or staining procedures.
Automatically generated - may contain errors
2 protocols using deparaffinization buffer
1
Immunohistochemical Staining of Mouse 4T1 Tumor Sections
2
Immunohistochemical Analysis of Mouse Tumors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse Hepa 1-6 and H22 tumor were fixed with 4% paraformaldehyde (Beyotime) and then transferred to 70% ethanol. IHC analysis was performed as previously described (50 (link)). In brief, paraffin-embedded sections were deparaffinized with deparaffinization buffer (Solarbio Life Science). Antigen retrieval and elimination of endogenous peroxidase activity were achieved with 10 mM citric acid buffer (pH 6.0) and 3% H2O2, respectively, followed by blocking and antibody incubation. Detection and visualization procedures were performed following the manufacturer’s protocol. The commercial antibodies used for IHC staining are listed in Supplemental Table 3 .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!