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Fp1487001kt

Manufactured by PerkinElmer
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The FP1487001KT is a laboratory equipment product from PerkinElmer. It is designed to perform specific tasks in a laboratory setting. The core function of this product is to provide an essential tool for laboratory operations, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Fp1488001kt, by PerkinElmer (3 mentions) Fp1497001kt, by PerkinElmer (2 mentions) Fp1494001kt, by PerkinElmer (2 mentions) Fp1495001kt, by PerkinElmer (2 mentions) Fp1496001kt, by PerkinElmer (2 mentions) Antigenfix, by Diapath (2 mentions) Antibody diluent, by Agilent Technologies (1 mentions) Rhrp520l, by Biocare Medical (1 mentions) Van gogh diluent, by Biocare Medical (1 mentions) Observer 2, by Zeiss (1 mentions) H 1200, by Vector Laboratories (1 mentions) Prolong gold antifade mountant, by Thermo Fisher Scientific (1 mentions) Rnascope probe, by Advanced Cell Diagnostics (1 mentions)

4 protocols using fp1487001kt

1

Immunohistochemical Analysis of ER and PR

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After the rehydration process, slides were immersed in water before being subjected to microwave treatment for antigen retrieval. For ER and PR staining, slides were placed in citrate buffer solution (pH 6.0) and microwaved. Slides were blocked with Antibody Diluent (Agilent, S302283-2). Samples were then incubated with primary antibodies. Tyramide signal amplificiation fluorophores (TSA) were used as the substrate to the HRP-conjugated antibody (Perkin Elmer, FP1497001KT, FP1487001KT). Slides were mounted using Vectashield Mounting Medium for Fluorescence with DAPI (Vector, H-1200). Primary antibodies: anti-ERα rabbit antibody diluted 1:500 (Millipore Sigma, 06-935); anti-PR rabbit antibody diluted 1:1000 (Abcam, ab131486); secondary antibodies: Mach 2 Rabbit HRP-polymer diluted 1:2 (Biocare Medical, RHRP520L) in Van Gogh Diluent (Biocare Medical, PD902L). ERα and PR antibodies were validated using B6C3F1 mouse uterus tissue (diestrus) by LaPlante et al.49 (link). Images were captured on a Zeiss Observer II with a ×40/1.4NA Plan-Apochromat DIC Oil objective.
Kanaya N., Chang G., Wu X., Saeki K., Bernal L., Shim H.J., Wang J., Warden C., Yamamoto T., Li J., Park J.S., Synold T., Vonderfecht S., Rakoff M., Neuhausen S.L, & Chen S. (2019). Single-cell RNA-sequencing analysis of estrogen- and endocrine-disrupting chemical-induced reorganization of mouse mammary gland. Communications Biology, 2, 406.
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2

Multiplex RNA Detection in Tumor Tissues

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Tumor samples were collected and fixed in 4% PFA for 24h at RT. Then samples were dehydrated using ethanol series followed by xylene and embedded in paraffin using standard procedures. Tissue blocks were cut in 5 μm-thick sections collected onto Superfrost™ Plus slides. Multiplex fluorescent in situ hybridization was performed using the RNAscope Multiplex Fluorescent V2 Assay kit (ACDBio 323100), reagents and probes according to manufacturer’s instructions. RNAscope® probes were designed commercially by the manufacturer and are available from Advanced Cell Diagnostics, Inc.. The following probes were used: Dll4 (#31997), Sox10 (#435931), Notch3 (#425171). Probes were then labelled with TSA opal 520 (PerkinElmer FP1487001KT), TSA opal 570 (PerkinElmer FP1488001KT) and TSA opal 690 (PerkinElmer FP1497001KT). Samples were counterstained with DAPI for 5 min and mounted with ProLong Gold Antifade Mountant (Thermofisher Scientific, #P36930).
Raha M., Wang G., Seidman M.M, & Glazer P.M. (1996). Mutagenesis by third-strand-directed psoralen adducts in repair-deficient human cells: high frequency and altered spectrum in a xeroderma pigmentosum variant.. Proceedings of the National Academy of Sciences of the United States of America, 93(7), 2941-2946.
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3

Multiplex RNA Visualization in Tissue Sections

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Experiments were performed using the RNAScope Multiplex Fluorescent V2 Assay kit (ACDBio 323100). Probes targeting intronic regions for Hs-Cd5l (ACDBio 850511), Mfa-Cd5l (ACDBio 873211), Mm-Cd5l (ACDBio 573271), Mm-Flt3 (ACDBio 487861), Mm-Xcr1 (ACDBio 562371), Mm-Mafb (ACDBio 438531) and Mm-Mgl2-O1 (ACDBio 822901) were custom-designed and synthesized. They were then labelled with TSA opal 520 (PerkinElmer FP1487001KT), TSA opal 540 (PerkinElmer FP1494001KT), TSA opal 570 (PerkinElmer FP1488001KT), TSA opal 620 (PerkinElmer FP1495001KT) or TSA opal 650 (PerkinElmer FP1496001KT). Tissues were fixed for 16 hours in AntigenFix (Diapath P0016), dehydrated and embedded in OCT as described above. Slices were pre-treated with hydrogen peroxide for 10 min and protease III for 20 min. The recommended Antigen retrieval step was not performed in order to preserve epitope integrity. Probes were hybridized and amplified according to the manufacturer’s instructions. Slides were then stained for protein markers as described above.
Guilliams M., Bonnardel J., Haest B., Vanderborght B., Wagner C., Remmerie A., Bujko A., Martens L., Thoné T., Browaeys R., De Ponti F.F., Vanneste B., Zwicker C., Svedberg F.R., Vanhalewyn T., Gonçalves A., Lippens S., Devriendt B., Cox E., Ferrero G., Wittamer V., Willaert A., Kaptein S.J., Neyts J., Dallmeier K., Geldhof P., Casaert S., Deplancke B., ten Dijke P., Hoorens A., Vanlander A., Berrevoet F., Van Nieuwenhove Y., Saeys Y., Saelens W., Van Vlierberghe H., Devisscher L, & Scott C.L. (2022). Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches. Cell, 185(2), 379-396.e38.
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4

Multiplex RNA Profiling in Tissue Sections

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Experiments were performed using the RNAScope Multiplex Fluorescent V2 Assay kit (ACDBio 323100). Probes targeting intronic regions for Hs-Cd5l (ACDBio 850511), Mfa-Cd5l (ACDBio 873211), Mm-Cd5l (ACDBio 573271), Mm-Flt3 (ACDBio 487861), Mm-Xcr1 (ACDBio 562371), Mm-Mafb (ACDBio 438531) and Mm-Mgl2-O1 (ACDBio 822901) were customdesigned and synthesized. They were then labelled with TSA opal 520 (PerkinElmer FP1487001KT), TSA opal 540 (PerkinElmer FP1494001KT), TSA opal 570 (PerkinElmer FP1488001KT), TSA opal 620 (PerkinElmer FP1495001KT) or TSA opal 650 (PerkinElmer FP1496001KT). Tissues were fixed for 16 hours in AntigenFix (Diapath P0016), dehydrated and embedded in OCT as described above. Slices were pre-treated with hydrogen peroxide for 10 min and protease III for 20 min. The recommended Antigen retrieval step was not performed in order to preserve epitope integrity. Probes were hybridized and amplified according to the manufacturer's instructions. Slides were then stained for protein markers as described above.
Guilliams M., Bonnardel J., Haest B., Vanderborght B., Bujko A., Martens L., Thoné T., Browaeys R., Ponti F.F.D., Remmerie A., Wagner C., Vanneste B., Zwicker C., Vanhalewyn T., Gonçalves A., Lippens S., Devriendt B., Cox E., Ferrero G., Wittamer V., Willaert A., Kaptein S.J.F., Neyts J., Dallmeier K., Geldhof P., Casaert S., Deplancke B., Dijke P.t., Hoorens A., Vanlander A., Berrevoet F., Nieuwenhove Y.V., Saeys Y., Saelens W., Vlierberghe H.V., Devisscher L., & Scott C.L. (2021). Spatial proteogenomics reveals distinct and evolutionarily-conserved hepatic macrophage niches.
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