20at hplc instrument

Six tablets were used from each sample batch, and the dissolution profile of each tablet was determined. We adopted the method outlined in the 2020 Chinese Pharmacopoeia to determine enteric-coated erythromycin tablet dissolution. A SOTAX AT 7X dissolution apparatus (SOTAX, Aesch, Switzerland) was used at a paddle speed of 50 rpm in a water bath at a temperature of 37°C. The samples were first dissolved in 0.1 mol/L hydrochloric acid solution for 60 min and then transferred to pH 5.5 phosphate buffer solution for dissolution. The time at which the coating began to dissolve was designated 0 min, and 5-mL samples were collected at 5, 7.5, 10, 12.5, 15, 17.5, 20, 22.5, and 25 min. After filtration through a 0.45-µm filter membrane, the dissolved amount was determined using a Shimadzu 20AT HPLC instrument (Shimadzu, Kyoto, Japan) with a Waters Xbridgeshiled RP column (4.6 mm × 150 mm, 3.5 µm; Waters Corporation, Milford, MA, United States). The mobile phase was A-acetonitrile (60:40), the column temperature was 45°C, the detection wavelength was 210 nm, and the reference solution contained approximately 0.25 mg/mL erythromycin A diluted with pH 5.5 phosphate buffer.

Six tablets were used from each sample batch, and the dissolution profile of each tablet was determined. We adopted the method outlined in the 2020 Chinese Pharmacopoeia to determine enteric-coated erythromycin tablet dissolution. A SOTAX AT 7X dissolution apparatus (SOTAX, Aesch, Switzerland) was used at a paddle speed of 50 rpm in a water bath at a temperature of 37°C. The samples were first dissolved in 0.1 mol/L hydrochloric acid solution for 60 min and then transferred to pH 5.5 phosphate buffer solution for dissolution. The time at which the coating began to dissolve was designated 0 min, and 5-mL samples were collected at 5, 7.5, 10, 12.5, 15, 17.5, 20, 22.5, and 25 min. After filtration through a 0.45-µm filter membrane, the dissolved amount was determined using a Shimadzu 20AT HPLC instrument (Shimadzu, Kyoto, Japan) with a Waters Xbridgeshiled RP column (4.6 mm × 150 mm, 3.5 µm; Waters Corporation, Milford, MA, United States). The mobile phase was A-acetonitrile (60:40), the column temperature was 45°C, the detection wavelength was 210 nm, and the reference solution contained approximately 0.25 mg/mL erythromycin A diluted with pH 5.5 phosphate buffer.