Pyrogallol

Roswell Park Memorial Institute 1640 (RPMI 1640), Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum, streptomycin, and penicillin were purchased from GIBCO (Grand Island, NY, USA). Lipopolysaccharide (LPS), Poly (I:C:), Pam3CSK4, 3-(4-5-dimethylathiaol-2-yl)-2-5-diphenyltetrazolium bromide (MTT), L-NAME (NO inhibitor), dimethyl sulfoxide (DMSO), sodium dodecyl sulfate (SDS), and ranitidine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Pyrogallol was purchased from MedChemExpress (Monmouth Junction, NJ, USA). Phosphate-buffered saline (PBS) was received from Samchun Pure Chemical Co. (Gyeonggi-do, Republic of Korea). TRIzol was purchased from Molecular Research Center, Inc. (Cincinnati, OH, USA). The cDNA synthesis kit was purchased from Thermo Fisher Scientific (Waltham, MA, USA), and primers for semiquantitative RT-PCR and quantitative real-time PCR were procured from Macrogen (Seoul, Republic of Korea). The PCR premix was received from Bio-D Inc. (Seoul, Republic of Korea). Antibodies against β-actin were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Total-protein and phospho-specific p50, p65, IκBα, AKT, Syk, Src, and Myc targeting antibodies were produced by Cell Signaling Technology (Beverly, MA, USA).

Pyrogallol (purity > 99.98%; HY‐N1579) (Figure 1A) and ML385 (HY‐100523) were obtained from MedChemExpress. The HO‐1 inhibitor ZnPP was purchased from AdooQ BioScience (Nanjing, China). MTT was purchased from Sigma‐Aldrich. DAPI (C1006) was obtained from Beyotime Biotechnology, Inc. (Shanghai, China). Recombinant human IFN‐β (#300‐02BC) was produced by Peprotech, Inc. (Rocky Hill, NJ, USA). Bead‐based multianalyte profiling kits for quantification of MIP‐1α, TNF‐α, MCP‐1, IL‐8, IP‐10, TRAIL, IL‐6, and RANTES were procured from Bio‐Rad Laboratories Inc. (Hercules, CA, USA). Antibodies are shown in Table S1.