NSCLC cells were suspended with binding buffer to prepare a single cell suspension. Cells were hatched with Annexin V‐FITC and PI solution (Sigma‐Aldrich). FACScan flow cytometry and CellQuest software were used to determine cell apoptosis rate.
Annexin 5 fitc and pi solution
Manufactured by Merck Group
Sourced in United States
Annexin V-FITC and PI solution is a laboratory reagent used to detect and quantify apoptosis, a form of programmed cell death. Annexin V is a protein that binds to phosphatidylserine, a phospholipid that is exposed on the cell surface during apoptosis. FITC (Fluorescein Isothiocyanate) is a fluorescent dye conjugated to Annexin V, allowing for the detection of apoptotic cells. Propidium Iodide (PI) is a DNA-binding dye that stains the nuclei of cells with compromised cell membranes, such as late apoptotic or necrotic cells. The combination of Annexin V-FITC and PI allows for the identification and quantification of different stages of cell death in a sample.
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Lab products found in correlation
2 protocols using annexin 5 fitc and pi solution
1
Apoptosis Quantification in NSCLC Cells
2
Assessing RA-cell Apoptosis by Annexin V-FITC and PI
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The RA-cells were treated with different concentrations of PG (0, 10, 20, and 50 µg/mL) and then cultured at a density of 1×106 cells/mL for 24 h. After washing the cells twice with cold-phosphate buffered saline (PBS), which were resuspended in a 1× binding buffer and then mixed with Annexin V-FITC and PI solution (Sigma-Aldrich, Louis, MO, USA) for 15 min at room temperature in darkness. Cells were finally examined by a flow cytometer (Thermo Scientific, Waltham, MA, USA).
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